CD278 antibody | C398.4A
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Hamster anti Mouse CD278, clone C398.4A recognizes CD278 also known as ICOS (inducible T cell co-stimulatory molecule) or H4. CD278 is a member of the CD28 family (Hutloff et al. 1999) which also contains the immune attenuator CTLA-4. CD278 expression is found following T cell activation (Yoshinaga et al. 1999) and is able to co-stimulate T cell proliferative responses. ICOS binds to ICOS ligand which is expressed on antigen presenting cells. This ICOS/ICOSL interaction activates PI3K/AKT, MAPK and NKFB signalling pathways in a manner distinct from that acitvated via CD28. In a larger focus ICOS signaling plays a role in several physiological processes such as T follicular helper cell induction and maturation, antibody affininty maturation and regulation of T helper cell inflammatory responses.
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Mouse T cell clone D10.G4.1
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch
- Shelf Life
- 18 months from date of despatch
- Shelf Life
- 18 months from date of despatch
More Information
- UniProt
- Q9WVS0 Related reagents
- Entrez Gene
- Icos Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0005887 integral to plasma membrane
- GO:0009897 external side of plasma membrane
- Regulatory
- For research purposes only
Applications of CD278 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/10 | 1/20 | |
| Flow Cytometry | 1/5 | 1/10 | |
| Flow Cytometry | 1/2 | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat F(ab')2 anti Hamster IgG:DyLight®549 | STAR104D549GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:DyLight®649 | STAR104D649GA | 0.1 mg | F IF | ||
| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | 0.1 mg | F IF WB | ||
| Goat F(ab')2 anti Hamster IgG:FITC | STAR104F | 0.4 mg | F | ||
| Goat anti Hamster IgG:Biotin | STAR79B | 0.8 mg | E F | ||
| Goat anti Hamster IgG:FITC | STAR79F | 0.7 mg | F | ||
| Goat anti Hamster IgG:HRP | STAR79P | 0.8 mg | E |
Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Hamster (Armenian) IgG Negative Control:FITC | MCA2356F | 0.1 mg | F | ||
| Hamster (Armenian) IgG Negative Control | MCA2356 | 0.25 mg | F | ||
| Hamster (Armenian) IgG Negative Control:RPE | MCA2356PE | 0.1 mg | F |
Product Specific References
Further Reading
-
Hutloff, A. et al. (1999) ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28.
Nature. 397 (6716): 263-6. -
Yoshinaga SK et al. (1999) T-cell co-stimulation through B7RP-1 and ICOS.
Nature. 402 (6763): 827-32.
