CD278 antibody | C398.4A

Hamster anti Mouse CD278

Product Type
Monoclonal Antibody
Clone
C398.4A
Isotype
IgG
Specificity
CD278

Product Code Applications Pack Size List Price Your Price Qty
MCA6051
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.25 mg loader
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loader
MCA6051GA
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
C F IP 0.1 mg loader
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loader
Search for Batch Specific Datasheets

Hamster anti Mouse CD278, clone C398.4A recognizes CD278 also known as ICOS (inducible T cell co-stimulatory molecule) or H4. CD278 is a member of the CD28 family (Hutloff et al. 1999) which also contains the immune attenuator CTLA-4. CD278 expression is found following T cell activation (Yoshinaga et al. 1999) and is able to co-stimulate T cell proliferative responses. ICOS binds to ICOS ligand which is expressed on antigen presenting cells. This ICOS/ICOSL interaction activates PI3K/AKT, MAPK and NKFB signalling pathways in a manner distinct from that acitvated via CD28. In a larger focus ICOS signaling plays a role in several physiological processes such as T follicular helper cell induction and maturation, antibody affininty maturation and regulation of T helper cell inflammatory responses.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse T cell clone D10.G4.1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

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Description Goat F(ab')2 anti Hamster IgG:Dylight®800
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Description Goat F(ab')2 anti Hamster IgG:FITC
Goat anti Hamster IgG:Biotin STAR79B E F 0.8 mg loader
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Description Goat anti Hamster IgG:Biotin
Goat anti Hamster IgG:FITC STAR79F F 0.7 mg loader
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Description Goat anti Hamster IgG:FITC
Goat anti Hamster IgG:HRP STAR79P E 0.8 mg loader
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Description Goat anti Hamster IgG:HRP

Description Product Code Applications Pack Size List Price Your Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 F 0.25 mg loader
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Description Hamster (Armenian) IgG Negative Control

Further Reading

  1. Hutloff, A. et al. (1999) ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28.
    Nature. 397 (6716): 263-6.
  2. Yoshinaga SK et al. (1999) T-cell co-stimulation through B7RP-1 and ICOS.
    Nature. 402 (6763): 827-32.

UniProt
Q9WVS0
Entrez Gene
Icos
GO Terms
GO:0005515 protein binding
GO:0005887 integral to plasma membrane
GO:0009897 external side of plasma membrane
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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