CD278 antibody | C398.4A
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Hamster anti Mouse CD278
- Product Type
- Monoclonal Antibody
- Clone
- C398.4A
- Isotype
- IgG
- Specificity
- CD278
Antibody Quality and 
Unrivaled 
| Hamster anti Mouse CD278, clone C398.4A recognizes CD278 also known as ICOS (inducible T cell co-stimulatory molecule) or H4. CD278 is a member of the CD28 family (Hutloff et al. 1999) which also contains the immune attenuator CTLA-4. CD278 expression is found following T cell activation (Yoshinaga et al. 1999) and is able to co-stimulate T cell proliferative responses. ICOS binds to ICOS ligand which is expressed on antigen presenting cells. This ICOS/ICOSL interaction activates PI3K/AKT, MAPK and NKFB signalling pathways in a manner distinct from that acitvated via CD28. In a larger focus ICOS signaling plays a role in several physiological processes such as T follicular helper cell induction and maturation, antibody affininty maturation and regulation of T helper cell inflammatory responses. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Human - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- Mouse T cell clone D10.G4.1
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/5 | 1/10 |
| Immunohistology - Frozen | |
||
| Immunoprecipitation | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
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| Goat F(ab')2 anti Hamster IgG:Dylight®800 | STAR104D800GA | F IF WB | 0.1 mg |
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| Goat anti Hamster IgG:FITC | STAR79F | F | 0.7 mg |
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| Goat anti Hamster IgG:HRP | STAR79P | E | 0.8 mg |
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| Hamster (Armenian) IgG Negative Control | MCA2356 | F | 0.25 mg |
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| Description | Hamster (Armenian) IgG Negative Control | ||||||
Further Reading
-
Hutloff, A. et al. (1999) ICOS is an inducible T-cell co-stimulator structurally and functionally related to CD28.
Nature. 397 (6716): 263-6. -
Yoshinaga SK et al. (1999) T-cell co-stimulation through B7RP-1 and ICOS.
Nature. 402 (6763): 827-32.
- UniProt
- Q9WVS0
- Entrez Gene
- Icos
- GO Terms
- GO:0005515 protein binding
- GO:0005887 integral to plasma membrane
- GO:0009897 external side of plasma membrane
View more products with CD278 specificity
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