CD278 antibody | C398.4A

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Hamster anti Mouse CD278

Product Type
Monoclonal Antibody
Clone
C398.4A
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA4718 C F IP 0.25 mg
Hamster anti Mouse CD278 antibody, clone C398.4A recognizes CD278, also known as Inducible T cell costimulator or ICOS, a CD28-superfamily costimulatory molecule. It is expressed on activated T cells and resting memory T cells. It enhances all the basic T cell responses to a foreign antigen, namely proliferation, secretion of lymphokines and up-regulation of molecules that mediate cell-cell interaction. It is essential for the efficient interaction between B and T cells and is involved in antibody responses to T cell dependent antigens. It up-regulates the synthesis of interleukin-10, but not interleukin-2 and prevents the apoptosis of pre-activated T cells. Hamster anti Mouse CD278 antibody, clone C398.4A has been reported to costimulate T cell activation and proliferation in vitro.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Mouse T cell clone D10.G4.1.
Approx. Protein Concentrations
IgG concentration 0.5mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9WVS0 Related reagents
Q9Y6W8 Related reagents
Q9R1T7 Related reagents
Entrez Gene
Icos Related reagents
Icos Related reagents
ICOS Related reagents
GO Terms
GO:0002517 T cell tolerance induction
GO:0005887 integral to plasma membrane
GO:0016337 cell-cell adhesion
GO:0031295 T cell costimulation
GO:0005515 protein binding
GO:0009897 external side of plasma membrane
GO:0005576 extracellular region
GO:0006955 immune response
Regulatory
For research purposes only

Applications of CD278 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/10
Immunohistology - Frozen
Immunoprecipitation
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat F(ab')2 anti Hamster IgG:DyLight®549 STAR104D549GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:DyLight®649 STAR104D649GA 0.1 mg F IF
Goat F(ab')2 anti Hamster IgG:Dylight®800 STAR104D800GA 0.1 mg F IF WB
Goat F(ab')2 anti Hamster IgG:FITC STAR104F 0.4 mg F
Goat anti Hamster IgG:Biotin STAR79B 0.8 mg E F
Goat anti Hamster IgG:FITC STAR79F 0.7 mg F
Goat anti Hamster IgG:HRP STAR79P 0.8 mg E

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Hamster (Armenian) IgG Negative Control MCA2356 0.25 mg F

Product Specific References

References for CD278 antibody

  1. Redoglia, V. et al. (1996) Characterization of H4: a mouse T lymphocyte activation molecule functionally associated with the CD3/T cell receptor.
    Eur J Immunol. 26 (11): 2781-9.
  2. Sakthivel, P. et al. (2014) Attenuation of immune-mediated influenza pneumonia by targeting the inducible co-stimulator (ICOS) molecule on T cells.
    PLoS One. 9 (7): e100970.
  3. Weber, J.P. et al. (2015) ICOS maintains the T follicular helper cell phenotype by down-regulating Krüppel-like factor 2.
    J Exp Med. 212 (2): 217-33.