Rat anti Mouse CD276 antibody, clone MJ8 recognizes mouse CD276, otherwise known as B7-H3, a ubiquitously expressed transmembrane glycoprotein and member of the B7 family of co-stimulatory molecules, which acts as both a positive and negative regulator of T-cell-mediated immune responses.

CD276 is highly expressed in bone during embryogenesis, and can be induced on dendritic cells and monocytes by inflammatory cytokines. CD276 has been implicated in the development of acute and chronic transplant rejection, and is reported to have therapeutic potential as a regulator of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy. In mice, CD276 has been linked with the development of pathogenic Th2 cells during the induction phase of allergic asthma (Nagashima et al. 2008).

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry

Flow Cytometry

Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A or BUF041B).