CD276 antibody | MJ8

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Rat anti Mouse CD276:RPE

Product Type
Monoclonal Antibody
Clone
MJ8
Isotype
IgG2b
Product Code Applications Pack Size List Price Quantity
MCA5646PE
Datasheet
F
SDS
100 Tests loader

Rat anti Mouse CD276 antibody, clone MJ8 recognizes mouse CD276, otherwise known as B7-H3, a ubiquitously expressed transmembrane glycoprotein and member of the B7 family of co-stimulatory molecules, which acts as both a positive and negative regulator of T-cell-mediated immune responses.

CD276 is highly expressed in bone during embryogenesis, and can be induced on dendritic cells and monocytes by inflammatory cytokines. CD276 has been implicated in the development of acute and chronic transplant rejection, and is reported to have therapeutic potential as a regulator of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy. In mice, CD276 has been linked with the development of pathogenic Th2 cells during the induction phase of allergic asthma (Nagashima et al. 2008).

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
5%Sucrose
Immunogen
Mouse IgG2a Fc-CD276 (aa 1-242).
Fusion Partners
Lymph node cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at +4oC.
DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
Q8VE98
Entrez Gene
Cd276
GO Terms
GO:0005102 receptor binding
GO:0016021 integral to membrane
GO:0009897 external side of plasma membrane
GO:0030501 positive regulation of bone mineralization
GO:0042130 negative regulation of T cell proliferation
GO:0045077 negative regulation of interferon-gamma biosynthetic process
GO:0045085 negative regulation of interleukin-2 biosynthetic process
GO:0045669 positive regulation of osteoblast differentiation
GO:0050728 negative regulation of inflammatory response
GO:0050776 regulation of immune response
Regulatory
For research purposes only

Applications of CD276 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A or BUF041B).

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
Mouse Seroblock FcR BUF041B F 0.5 mg loader

Product Specific References

References for CD276 antibody

  1. Nagashima, O. et al. (2008) B7-H3 contributes to the development of pathogenic Th2 cells in a murine model of asthma.
    J Immunol. 181 (6): 4062-71.

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