CD276 antibody | MJ18
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F ResetRat anti Mouse CD276
- Product Type
- Monoclonal Antibody
- Clone
- MJ18
- Isotype
- IgG1
- Specificity
- CD276
Antibody Quality and 
Unrivaled 
| Rat anti Mouse CD276 antibody, clone MJ18 specifically recognizes mouse CD276, otherwise known as B7-H3, a ubiquitously expressed transmembrane glycoprotein and member of the B7 family of co-stimulatory molecules, which acts as both a positive and negative regulator of T-cell-mediated immune responses. CD276 is highly expressed in bone during embryogenesis, and can be induced on dendritic cells and monocytes by inflammatory cytokines. CD276 has been implicated in the development of acute and chronic transplant rejection, and is reported to have therapeutic potential as a regulator of cell-mediated immune responses to cancer, particularly in conjunction with anti-angiogenic therapy. In mice, CD276 has been linked with the development of pathogenic Th2 cells during the induction phase of allergic asthma and have shown that Rat anti Mouse CD276 antibody, clone MJ18 inhibits stimulated CD4+ T-cell proliferation (Nagashima et al. 2008). |
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- Mouse IgG2a Fc-CD276 (aa 1-242).
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Lymph node cells from immunised Sprague Dawley rats were fused with cells of the P3U1 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
1/25 | 1/200 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for CD276 antibody
-
Nagashima, O. et al. (2008) B7-H3 contributes to the development of pathogenic Th2 cells in a murine model of asthma.
J Immunol. 181 (6): 4062-71. -
Fukushima, A. et al. (2007) B7-H3 regulates the development of experimental allergic conjunctivitis in mice.
Immunol Lett. 113 (1): 52-7.
- Synonyms
- B7-H3
- RRID
- AB_10843423
- UniProt
- Q8VE98
- Entrez Gene
- Cd276
- GO Terms
- GO:0005102 receptor binding
- GO:0016021 integral to membrane
- GO:0009897 external side of plasma membrane
- GO:0030501 positive regulation of bone mineralization
- GO:0042130 negative regulation of T cell proliferation
- GO:0045077 negative regulation of interferon-gamma biosynthetic process
- GO:0045085 negative regulation of interleukin-2 biosynthetic process
- GO:0045669 positive regulation of osteoblast differentiation
- GO:0050728 negative regulation of inflammatory response
- View More GO Terms
- GO:0050776 regulation of immune response
View more products with CD276 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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