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CD273 antibody | TY25

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Rat anti Mouse CD273:FITC

Product Type
Monoclonal Antibody
Clone
TY25
Isotype
IgG2a
Specificity
CD273

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Product Code Applications Pack Size List Price Your Price Qty
MCA2465F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Rat anti Mouse CD273 antibody, clone TY25 recognises mouse CD273, also known as B7-dendritic cell (B7-DC), or programmed death ligand-2 (PD-L2). CD273 is a 247 amino acid, ~42 kDa member of the B7 family. The expression of CD273 is restricted to a subpopulation of dendritic cells (DCs), and DCs and macrophages that have been stimulated with interferon gamma, GM-CSF or IL-4.

CD273 has been identified as a ligand for PD-1(CD279), along with another B7 family member B7-H1 (CD274). CD273 and B7-H1 co-stimulate or inhibit T cell proliferation and cytokine production (Schmidt et al.2015).

Rat anti Mouse CD273 antibody, clone TY25 is reported to block the binding of CD273 (B7-DC) to it's receptor (Matsumoto et al. 2004).

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
B7-DC transfected RAW264.7 cells.
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Lymph node cells from immunized SD rats were fused with the cells of the P3U1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
List Price Your Price
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Description Rat IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

Source Reference

  1. Yamazaki, T. et al. (2002) Expression of programmed death 1 ligands by murine T cells and APC.
    J Immunol. 169 (10): 5538-45.

References for CD273 antibody

  1. Kanai, T. et al. (2003) Blockade of B7-H1 suppresses the development of chronic intestinal inflammation.
    J Immunol. 171 (8): 4156-63.
  2. Matsumoto, K. et al. (2004) B7-DC regulates asthmatic response by an IFN-gamma-dependent mechanism.
    J Immunol. 172 (4): 2530-41.
  3. Yamazaki, T. et al. (2005) Blockade of B7-H1 on macrophages suppresses CD4+ T cell proliferation by augmenting IFN-gamma-induced nitric oxide production.
    J Immunol. 175: 1586-92.
  4. Furuhashi K et al. (2012) Mouse lung CD103+ and CD11b high dendritic cells preferentially induce distinct CD4+ T-cell responses.
    Am J Respir Cell Mol Biol. 46 (2): 165-72.
  5. Lopez-Medina, M. et al. (2015) Salmonella induces PD-L1 expression in B cells.
    Immunol Lett. 167 (2): 131-40.
  6. López-Medina, M. et al. (2015) Salmonella impairs CD8 T cell response through PD-1: PD-L axis.
    Immunobiology. 220 (12): 1369-80.

Immunohistology - Frozen

Synonyms
PD-L2
RRID
AB_905916
UniProt
Q9WUL5
Entrez Gene
Pdcd1lg2
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0042102 positive regulation of T cell proliferation
GO:0042130 negative regulation of T cell proliferation

MCA2465F

150835

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