CD265 antibody | LOB14-8
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Rat anti Mouse CD265 antibody, clone LOB14-8 recognizes murine receptor activator of NF Kappa-B (RANK), otherwise known as CD265. CD265 is a member of the tumour necrosis factor (TNF) receptor superfamily and plays an important role in lymph node development and osteoclast differentiation.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- RANK-Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized lou rat were fuzed with cells of the mouse NS-1 myeloma cel line.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O35305
- Entrez Gene
- Tnfrsf11a
- GO Terms
- GO:0001503 ossification
- GO:0016021 integral to membrane
- GO:0005031 tumor necrosis factor receptor activity
- GO:0046872 metal ion binding
- GO:0048535 lymph node development
- GO:0032496 response to lipopolysaccharide
- GO:0034612 response to tumor necrosis factor
- GO:0060086 circadian temperature homeostasis
- GO:0060749 mammary gland alveolus development
- GO:0070555 response to interleukin-1
- GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
- Regulatory
- For research purposes only
Applications of CD265 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 20ug/ml | ||
| Flow Cytometry | 20ug/ml | ||
| Immunoprecipitation | 20ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 20ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
References for CD265 antibody
-
García-Pérez, M.A. et al. (2006) Alterations in the phenotype and function of immune cells in ovariectomy-induced osteopenic mice.
Hum Reprod. 21:880-7. -
Hoshino-Negishi, K. et al. (2019) Role of Anti-Fractalkine Antibody in Suppression of Joint Destruction by Inhibiting Migration of Osteoclast Precursors to the Synovium in Experimental Arthritis.
Arthritis Rheumatol. 71 (2): 222-31.
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