Rat anti Mouse CD265 antibody, clone LOB14-8 recognizes murine receptor activator of NF Kappa-B (RANK), otherwise known as CD265. CD265 is a member of the tumour necrosis factor (TNF) receptor superfamily and plays an important role in lymph node development and osteoclast differentiation.
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- RANK-Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized lou rat were fuzed with cells of the mouse NS-1 myeloma cel line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
integral to membrane
tumor necrosis factor receptor activity
metal ion binding
lymph node development
response to lipopolysaccharide
response to tumor necrosis factor
circadian temperature homeostasis
mammary gland alveolus development
response to interleukin-1
positive regulation of fever generation by positive regulation of prostaglandin secretion
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of CD265 antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 20ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
Negative Isotype Controls Available
Product Specific References
References for CD265 antibody
García-Pérez, M.A. et al. (2006) Alterations in the phenotype and function of immune cells in ovariectomy-induced osteopenic mice.
Hum Reprod. 21:880-7.
Hoshino-Negishi, K. et al. (2019) Role of Anti-Fractalkine Antibody in Suppression of Joint Destruction by Inhibiting Migration of Osteoclast Precursors to the Synovium in Experimental Arthritis.
Arthritis Rheumatol. 71 (2): 222-31.
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