CD265 antibody | LOB14-8
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Rat anti Mouse CD265 antibody, clone LOB14-8 recognizes murine receptor activator of NF Kappa-B (RANK), otherwise known as CD265. CD265 is a member of the tumour necrosis factor (TNF) receptor superfamily and plays an important role in lymph node development and osteoclast differentiation.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
- Buffer Solution
- TRIS buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
- Immunogen
- RANK-Fc fusion protein.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Cells from immunized lou rat were fuzed with cells of the mouse NS-1 myeloma cel line.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O35305
- Entrez Gene
- Tnfrsf11a
- GO Terms
- GO:0001503 ossification
- GO:0016021 integral to membrane
- GO:0005031 tumor necrosis factor receptor activity
- GO:0046872 metal ion binding
- GO:0048535 lymph node development
- GO:0032496 response to lipopolysaccharide
- GO:0034612 response to tumor necrosis factor
- GO:0060086 circadian temperature homeostasis
- GO:0060749 mammary gland alveolus development
- GO:0070555 response to interleukin-1
- GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
- Regulatory
- For research purposes only
Applications of CD265 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 20ug/ml | ||
| Flow Cytometry | 20ug/ml | ||
| Immunoprecipitation | 20ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 20ul of the suggested working dilution to label 106 cells in 100ul.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) | STAR131A | C E P WB | 1 ml |
|
|
| Goat anti Rat IgG:Biotin (Mouse Adsorbed) | STAR131B | C E IF P WB | 0.5 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:Dylight®800 | STAR16D800GA | F IF WB | 0.1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:FITC | STAR17B | F | 1 mg |
|
|
| Rabbit F(ab')2 anti Rat IgG:HRP | STAR21B | C E P RE | 1 mg |
|
|
| Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) | STAR69 | F | 0.5 ml |
|
|
| Goat anti Rat IgG:DyLight®650 (Mouse Adsorbed) | STAR71D650 | F IF | 0.1 mg |
|
|
| Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) | STAR71D800GA | F IF WB | 0.1 mg |
|
|
| Goat anti Rat IgG:HRP (Mouse Adsorbed) | STAR72 | C E P | 0.5 mg |
|
|
| Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) | STAR73 | F | 0.5 ml |
|
Negative Isotype Controls Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control | MCA1212 | E F | 1 ml |
|
Product Specific References
References for CD265 antibody
-
García-Pérez, M.A. et al. (2006) Alterations in the phenotype and function of immune cells in ovariectomy-induced osteopenic mice.
Hum Reprod. 21:880-7. -
Hoshino-Negishi, K. et al. (2019) Role of Anti-Fractalkine Antibody in Suppression of Joint Destruction by Inhibiting Migration of Osteoclast Precursors to the Synovium in Experimental Arthritis.
Arthritis Rheumatol. 71 (2): 222-31.
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