CD265 antibody | LOB14-8

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Rat anti Mouse CD265

Product Type
Monoclonal Antibody
Clone
LOB14-8
Isotype
IgG2a
Product Code Applications Pack Size List Price Quantity
MCA2085
Datasheet
E F IP
MSDS
0.25 mg loader

Rat anti Mouse CD265 antibody, clone LOB14-8 recognizes murine receptor activator of NF Kappa-B (RANK), otherwise known as CD265. CD265 is a member of the tumour necrosis factor (TNF) receptor superfamily and plays an important role in lymph node development and osteoclast differentiation.

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution
TRIS buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
RANK-Fc fusion protein.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Cells from immunized lou rat were fuzed with cells of the mouse NS-1 myeloma cel line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
O35305
Entrez Gene
Tnfrsf11a
GO Terms
GO:0001503 ossification
GO:0016021 integral to membrane
GO:0005031 tumor necrosis factor receptor activity
GO:0046872 metal ion binding
GO:0048535 lymph node development
GO:0032496 response to lipopolysaccharide
GO:0034612 response to tumor necrosis factor
GO:0060086 circadian temperature homeostasis
GO:0060749 mammary gland alveolus development
GO:0070555 response to interleukin-1
GO:0071812 positive regulation of fever generation by positive regulation of prostaglandin secretion
Regulatory
For research purposes only

Applications of CD265 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 20ug/ml
Flow Cytometry 20ug/ml
Immunoprecipitation 20ug/ml
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 20ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A C E P WB 1 ml loader
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B C E IF P WB 0.5 mg loader
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA F IF WB 0.1 mg loader
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B F 1 mg loader
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B C E P RE 1 mg loader
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 F 0.5 ml loader
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA F IF WB 0.1 mg loader
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 C E P 0.5 mg loader
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 F 0.5 ml loader

Negative Isotype Controls Available

Description Product Code Applications Pack Size List Price Quantity
Rat IgG2a Negative Control MCA1212 E F 1 ml loader

Product Specific References

References for CD265 antibody

  1. García-Pérez, M.A. et al. (2006) Alterations in the phenotype and function of immune cells in ovariectomy-induced osteopenic mice.
    Hum Reprod. 21:880-7.
  2. Hoshino-Negishi, K. et al. (2019) Role of Anti-Fractalkine Antibody in Suppression of Joint Destruction by Inhibiting Migration of Osteoclast Precursors to the Synovium in Experimental Arthritis.
    Arthritis Rheumatol. 71 (2): 222-31.