CD25 antibody | PC61.5.3

Rat anti Mouse CD25:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
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Rat anti Mouse CD25 antibody, clone PC61.5.3 reacts with the low affinity alpha chain of the interleukin-2 receptor present on activated T and B cells in mice. Rat anti Mouse CD25 antibody, clone PC61.5.3 is reported to inhibit IL-2 binding and IL-2 dependent proliferation.

Target Species
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine Serum Albumin
B6.1 CTL cell line.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Fusion Partners
Spleen cells from immunized OFA rats were fused with cells of the P3X63Ag8.653 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
Description Mouse Seroblock FcR

References for CD25 antibody

  1. Moreau, J.L. et al. (1987) Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site.
    Eur J Immunol. 17 (7): 929-35.
  2. Hashimoto, N. et al. (1986) Dissociation of interleukin 2-dependent and -independent B cell proliferation with monoclonal anti-interleukin 2 receptor antibody.
    Eur J Immunol. 16 (3): 317-20.
  3. Lowenthal, J.W. et al. (1985) High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61.
    J Immunol. 135 (6): 3988-94.
  4. Ceredig, R. et al. (1985) Expression of interleukin-2 receptor as a differentiation marker on intrathymic stem cells.
    Nature 314: 98-100.
  5. Yaqoob, P. & Calder, P.C. (1997) Glutamine requirement of proliferating T lymphocytes.
    Nutrition. 13 (7-8): 646-51.
  6. Scotland, R.S. et al. (2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.
    Blood. 118 (22): 5918-27.
  7. Karali, D. et al. (2016) T cell regulation by Phlomis lanata protein extracts in mice.
    Pharm Biol. 54 (2): 207-14.
  8. Szuster-Ciesielska, A. et al. (2019) Immunogenic Evaluation of Ribosomal P-Protein Antigen P0, P1, and P2 and Pentameric Protein Complex P0-(P1-P2)2 of Plasmodium falciparum in a Mouse Model.
    J Immunol Res. 2019: 9264217.
  9. View The Latest Product References
  10. Curina, G. et al. (2018) Evaluation of immune responses in mice and sheep inoculated with a live attenuated Brucella melitensis. REV1 vaccine produced in bioreactor.
    Vet Immunol Immunopathol. 198: 44-53.
  11. Arad, T. et al. (2021) CD200 -dependent and -independent immune-modulatory functions of neural stem cells.
    Stem Cell Res. 56: 102559.

IL-2R Alpha Chain
Entrez Gene
GO Terms
GO:0016021 integral to membrane
GO:0004911 interleukin-2 receptor activity
GO:0006924 activation-induced cell death of T cells
GO:0009897 external side of plasma membrane
GO:0042104 positive regulation of activated T cell proliferation
GO:0042130 negative regulation of T cell proliferation
GO:0043065 positive regulation of apoptosis
GO:0046013 regulation of T cell homeostatic proliferation
GO:0050728 negative regulation of inflammatory response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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