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CD21/CD35 antibody | 7E9

Rat anti Mouse CD21/CD35:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.5 mg loader
List Price Your Price

Rat anti Mouse CD21/CD35, clone 7E9 recognizes CD21/CD35 also known as CR2/CR1. CD21, also known as complement receptor 2 (CR2) and CD35, also known as complement receptor 1 (CR1) bind the activated products of C3 and C4. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81. CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages and activated granulocytes and they constitute the principle receptors for uptake and retention of immune complexes. In their absence, memory B-cell survival is markedly impaired.

Target Species
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
Guaranteed for 12 months from the date of despatch or until the date of expiry, whichever comes first. Please see label for expiry date.

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
List Price Your Price
Description Rat IgG2a Negative Control:FITC

References for CD21/CD35 antibody

  1. Kulik, L. et al. (2015) A new mouse anti-mouse complement receptor type 2 and 1 (CR2/CR1) monoclonal antibody as a tool to study receptor involvement in chronic models of immune responses and disease.
    Mol Immunol. 63 (2): 479-88.


Western Blotting

Entrez Gene
GO Terms
GO:0016021 integral to membrane
GO:0004875 complement receptor activity
GO:0006958 complement activation, classical pathway
GO:0009897 external side of plasma membrane
GO:0042113 B cell activation
GO:0045087 innate immune response
View more products with CD21 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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