CD21/CD35 antibody | 7E9

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Rat anti Mouse CD21/CD35:FITC

Rat anti Mouse CD21/CD35:RPE

Product Type
Monoclonal Antibody
Clone
7E9
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6043F F 0.5 mg
MCA6043PE F 0.2 mg
Rat anti Mouse CD21/CD35, clone 7E9 recognizes CD21/CD35 also known as CR2/CR1. CD21, also known as complement receptor 2 (CR2) and CD35, also known as complement receptor 1 (CR1) bind the activated products of C3 and C4. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81.CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages and activated granulocytes and they constitute the principle receptors for uptake and retention of immune complexes. In their absence, memory B-cell survival is markedly impaired.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by affinity chromatography
Preparation
Purified IgG prepared by affinity chromatography
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
CD35/CFA
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Approx. Protein Concentrations
IgG concentration 0.2 mg/ml

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch
Shelf Life
18 months from date of despatch

More Information

UniProt
P19070 Related reagents
Entrez Gene
Cr2 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004875 complement receptor activity
GO:0006958 complement activation, classical pathway
GO:0009897 external side of plasma membrane
GO:0042113 B cell activation
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of CD21/CD35 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/20
Flow Cytometry 1/5 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
Flow Cytometry
Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F