CD21/CD35 antibody | 7E9
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Rat anti Mouse CD21/CD35, clone 7E9 recognizes CD21/CD35 also known as CR2/CR1. CD21, also known as complement receptor 2 (CR2) and CD35, also known as complement receptor 1 (CR1) bind the activated products of C3 and C4. CD21/CD35 forms part of the B-cell antigen receptor complex with CD19 and CD81.CD21/CD35 is primarily expressed on B lymphocytes, mast cells, follicular dendritic cells, macrophages and activated granulocytes and they constitute the principle receptors for uptake and retention of immune complexes. In their absence, memory B-cell survival is markedly impaired.
Product Details
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Product Form
- Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography
- Preparation
- Purified IgG prepared by affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- CD35/CFA
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Approx. Protein Concentrations
- IgG concentration 0.2 mg/ml
Storage Information
- Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use. - Storage
- Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use. - Shelf Life
- 18 months from date of despatch
- Shelf Life
- 18 months from date of despatch
More Information
- UniProt
- P19070 Related reagents
- Entrez Gene
- Cr2 Related reagents
- GO Terms
- GO:0016021 integral to membrane
- GO:0004875 complement receptor activity
- GO:0006958 complement activation, classical pathway
- GO:0009897 external side of plasma membrane
- GO:0042113 B cell activation
- GO:0045087 innate immune response
- Regulatory
- For research purposes only
Applications of CD21/CD35 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry | 1/20 | ||
| Flow Cytometry | 1/5 | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
- Flow Cytometry
- Use 10 μl of the suggested working dilution to label 1x106 cells in 100ul
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Negative Isotype Controls Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Rat IgG2a Negative Control:FITC | MCA1212F | 100 Tests | F | ||
| Rat IgG2a Negative Control:RPE | MCA1212PE | 100 Tests | F |
