CD205 antibody | NLDC-145

Rat anti Mouse CD205

Product Type
Monoclonal Antibody
Clone
NLDC-145
Isotype
IgG2a
Specificity
CD205

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MCA949
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C F 2 ml loader
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Rat anti Mouse CD205, clone NLDC-145 recognizes DEC-205, a ~205 kDa integral membrane glycoprotein expressed by mouse dendritic cells and thymic epithelial cells. It is absent in bone marrow cells, all blood cells and freshly isolated macrophages from the peritoneal cavity. A subpopulation of the latter becomes positive in mice previously stimulated with thioglycollate. It is also absent on follicular dendritic cells of B cell follicles.

The antigen is found on la positive interdigitating cells in T cell areas of all secondary lymphoid organs. It is also present on veiled cells. In non-lymphoid organs, the antigen is only found on Langerhans cells of the skin but cross reactions with la positive thymic epithelial cells and intestinal villus epithelial cells are also observed.

DEC-205 is expressed at the cell surface of unfixed cells but can also be found in the cytoplasm of fixed cells.

Target Species
Mouse
Product Form
Tissue Culture Supernatant - liquid
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Mouse lymph node stroma.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry
Immunohistology - Frozen 1/10 1/25
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

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Source Reference

  1. Kraal, G. et al. (1986) Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody.
    J Exp Med. 163 (4): 981-97.

References for CD205 antibody

  1. Krepler, C. et al. (2004) CpG oligonucleotides elicit antitumor responses in a human melanoma NOD/SCID xenotransplantation model.
    J Invest Dermatol. 122 (2): 387-91.
  2. Noda, S. et al. (2006) Cytomegalovirus MCK-2 controls mobilization and recruitment of myeloid progenitor cells to facilitate dissemination.
    Blood. 107 (1): 30-8.
  3. Takenaka, N. et al. (2011) Overexpression of phospholipase Cε in keratinocytes upregulates cytokine expression and causes dermatitis with acanthosis and T-cell infiltration.
    Eur J Immunol. 41: 202-13.
  4. Di Rosa, F. et al. (2000) Short-lived immunization site inflammation in self-limited active experimental allergic encephalomyelitis.
    Int Immunol. 12: 711-9.
  5. Doepping, S. et al. (2007) Selective 5-lipoxygenase expression in Langerhans cells and impaired dendritic cell migration in 5-LO-deficient mice reveal leukotriene action in skin.
    J Invest Dermatol. 127: 1692-700.
  6. Kronin, V. et al. (2000) DEC-205 as a marker of dendritic cells with regulatory effects on CD8 T cell responses.
    Int Immunol. 12: 731-5.
  7. Padilla, J. et al. (2005) IL-13 regulates the immune response to inhaled antigens.
    J Immunol. 174: 8097-105.
  8. Palumbo, M.O. et al. (2006) Isolation and characterization of proinsulin-producing medullary thymic epithelial cell clones.
    Diabetes. 55: 2595-601.
  9. View The Latest Product References
  10. Serafini, B. et al. (2000) Intracerebral recruitment and maturation of dendritic cells in the onset and progression of experimental autoimmune encephalomyelitis.
    Am J Pathol. 157: 1991-2002.
  11. Wilson, H.L. and O'Neill, H.C. (2003) Identification of differentially expressed genes representing dendritic cell precursors and their progeny.
    Blood. 102: 1661-9.
  12. Barclay, A.N. & Ward, H.A. (1982) Purification and chemical characterisation of membrane glycoproteins from rat thymocytes and brain, recognised by monoclonal antibody MRC OX 2.
    Eur J Biochem. 129 (2): 447-58.
  13. Jiang, W. et al. (1995) The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing.
    Nature. 375 (6527): 151-5.
  14. Swiggard, W.J. et al. (1995) DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence.
    Cell Immunol. 165 (2): 302-11.
  15. Inaba, K. et al. (1995) Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes.
    Cell Immunol. 163 (1): 148-56.
  16. Wolf AJ et al. (2007) Mycobacterium tuberculosis infects dendritic cells with high frequency and impairs their function in vivo.
    J Immunol. 179 (4): 2509-19.
  17. Schliemann, C. et al. (2010) In vivo biotinylation of the vasculature in B-cell lymphoma identifies BST-2 as a target for antibody-based therapy.
    Blood. 115: 736-44.
  18. Jobe O et al. (2009) Immunization with radiation-attenuated Plasmodium berghei sporozoites induces liver cCD8alpha+DC that activate CD8+T cells against liver-stage malaria.
    PLoS One. 4 (4): e5075.
  19. Zhang, S.S. et al. (2008) Plasminogen activator Pla of Yersinia pestis utilizes murine DEC-205 (CD205) as a receptor to promote dissemination.
    J Biol Chem. 283: 31511-21.
  20. Mohan, J. et al. (2005) Skin-derived dendritic cells acquire and degrade the scrapie agent following in vitro exposure.
    Immunology. 116: 122-33.
  21. Jin, X. et al. (2014) Long-Term Persistence of Functional Thymic Epithelial Progenitor Cells In Vivo under Conditions of Low FOXN1 Expression.
    PLoS One. 9: e114842.
  22. Damian-Morales, G. et al. (2016) The HPV16 E7 Oncoprotein Disrupts Dendritic Cell Function and Induces the Systemic Expansion of CD11b(+)Gr1(+) Cells in a Transgenic Mouse Model.
    Biomed Res Int. 2016: 8091353.
  23. Wu Y et al. (2016) Effect of Aging on Periodontal Inflammation, Microbial Colonization, and Disease Susceptibility.
    J Dent Res. 95 (4): 460-6.
  24. Kobayashi, A. et al. (2015) AG490, a Jak2 inhibitor, suppressed the progression of murine ovarian cancer.
    Eur J Pharmacol. 766: 63-75.
  25. Xiao, W. et al. (2015) FOXO1 deletion reduces dendritic cell function and enhances susceptibility to periodontitis.
    Am J Pathol. 185 (4): 1085-93.
  26. Charmoy, M. et al. (2010) Neutrophil-derived CCL3 is essential for the rapid recruitment of dendritic cells to the site of Leishmania major inoculation in resistant mice.
    PLoS Pathog. 6 (2): e1000755.
  27. Suárez-Álvarez, R.O. et al. (2019) Dimorphism and Dissemination of Histoplasma capsulatum in the Upper Respiratory Tract after Intranasal Infection of Bats and Mice with Mycelial Propagules.
    Am J Trop Med Hyg. 101 (3): 716-723.
  28. Singh, T. et al. (2019) Loss of MafA and MafB expression promotes islet inflammation.
    Sci Rep. 9 (1): 9074.

Flow Cytometry

Immunohistology - Frozen

Synonyms
DEC205
RRID
AB_322018
UniProt
Q60767
Entrez Gene
Ly75
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005529 sugar binding
GO:0006897 endocytosis
GO:0009897 external side of plasma membrane
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