CD205 antibody | NLDC-145

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Rat anti Mouse CD205:Alexa Fluor® 488

Rat anti Mouse CD205:Alexa Fluor® 647

Rat anti Mouse CD205:FITC

Rat anti Mouse CD205

Product Type
Monoclonal Antibody
Clone
NLDC-145
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA949A488T F 25 Tests/0.25ml
MCA949A488 F 100 Tests/1ml
MCA949A647 F 100 Tests/1ml
MCA949FT F 25 µg
MCA949F F 0.1 mg
MCA949 C F 2 ml
Rat anti Mouse CD205, clone NLDC-145 recognizes DEC-205, a ~205 kDa integral membrane glycoprotein expressed by mouse dendritic cells and thymic epithelial cells. It is absent in bone marrow cells, all blood cells and freshly isolated macrophages from the peritoneal cavity. A subpopulation of the latter becomes positive in mice previously stimulated with thioglycollate. It is also absent on follicular dendritic cells of B cell follicles.

The antigen is found on la positive interdigitating cells in T cell areas of all secondary lymphoid organs. It is also present on veiled cells. In non-lymphoid organs, the antigen is only found on Langerhans cells of the skin but cross reactions with la positive thymic epithelial cells and intestinal villus epithelial cells are also observed.

DEC-205 is expressed at the cell surface of unfixed cells but can also be found in the cytoplasm of fixed cells.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 488 - liquid
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Tissue Culture Supernatant - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Preparation
Tissue Culture Supernatant containing 0.2M Tris/HCl pH7.4 and 5-10% foetal calf serum
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09% Sodium Azide
1%Bovine Serum Albumin
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Mouse lymph node stroma.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q60767 Related reagents
Entrez Gene
Ly75 Related reagents
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0005529 sugar binding
GO:0006897 endocytosis
GO:0009897 external side of plasma membrane
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of CD205 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry Neat 1/10
Flow Cytometry
Immunohistology - Frozen 1/10 1/25
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 488 MCA1212A488 100 Tests/1ml F
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 100 Tests/1ml F
Rat IgG2a Negative Control:FITC MCA1212F 100 Tests F
Rat IgG2a Negative Control MCA1212 1 ml E F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Application Based External Images

Flow Cytometry

Immunohistology - Frozen

Product Specific References

Source Reference

  1. Kraal, G. et al. (1986) Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody.
    J Exp Med. 163 (4): 981-97.

References for CD205 antibody

  1. Barclay, A.N. & Ward, H.A. (1982) Purification and chemical characterisation of membrane glycoproteins from rat thymocytes and brain, recognised by monoclonal antibody MRC OX 2.
    Eur J Biochem. 129 (2): 447-58.
  2. Jiang, W. et al. (1995) The receptor DEC-205 expressed by dendritic cells and thymic epithelial cells is involved in antigen processing.
    Nature. 375 (6527): 151-5.
  3. Swiggard, W.J. et al. (1995) DEC-205, a 205-kDa protein abundant on mouse dendritic cells and thymic epithelium that is detected by the monoclonal antibody NLDC-145: purification, characterization, and N-terminal amino acid sequence.
    Cell Immunol. 165 (2): 302-11.
  4. Inaba, K. et al. (1995) Tissue distribution of the DEC-205 protein that is detected by the monoclonal antibody NLDC-145. I. Expression on dendritic cells and other subsets of mouse leukocytes.
    Cell Immunol. 163 (1): 148-56.
  5. Wolf AJ et al. (2007) Mycobacterium tuberculosis infects dendritic cells with high frequency and impairs their function in vivo.
    J Immunol. 179 (4): 2509-19.
  6. Schliemann, C. et al. (2010) In vivo biotinylation of the vasculature in B-cell lymphoma identifies BST-2 as a target for antibody-based therapy.
    Blood. 115: 736-44.
  7. Jobe O et al. (2009) Immunization with radiation-attenuated Plasmodium berghei sporozoites induces liver cCD8alpha+DC that activate CD8+T cells against liver-stage malaria.
    PLoS One. 4 (4): e5075.
  8. Zhang, S.S. et al. (2008) Plasminogen activator Pla of Yersinia pestis utilizes murine DEC-205 (CD205) as a receptor to promote dissemination.
    J Biol Chem. 283: 31511-21.
  9. Mohan, J. et al. (2005) Skin-derived dendritic cells acquire and degrade the scrapie agent following in vitro exposure.
    Immunology. 116: 122-33.
  10. Jin, X. et al. (2014) Long-Term Persistence of Functional Thymic Epithelial Progenitor Cells In Vivo under Conditions of Low FOXN1 Expression.
    PLoS One. 9: e114842.
  11. Damian-Morales, G. et al. (2016) The HPV16 E7 Oncoprotein Disrupts Dendritic Cell Function and Induces the Systemic Expansion of CD11b(+)Gr1(+) Cells in a Transgenic Mouse Model.
    Biomed Res Int. 2016: 8091353.
  12. Wu Y et al. (2016) Effect of Aging on Periodontal Inflammation, Microbial Colonization, and Disease Susceptibility.
    J Dent Res. 95 (4): 460-6.
  13. Kobayashi, A. et al. (2015) AG490, a Jak2 inhibitor, suppressed the progression of murine ovarian cancer.
    Eur J Pharmacol. 766: 63-75.
  14. Xiao, W. et al. (2015) FOXO1 deletion reduces dendritic cell function and enhances susceptibility to periodontitis.
    Am J Pathol. 185 (4): 1085-93.
  15. Charmoy, M. et al. (2010) Neutrophil-derived CCL3 is essential for the rapid recruitment of dendritic cells to the site of Leishmania major inoculation in resistant mice.
    PLoS Pathog. 6 (2): e1000755.