CD204 antibody | 2F8
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Rat anti Mouse CD204:Alexa Fluor® 700
- Product Type
- Monoclonal Antibody
|Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Rat anti Mouse CD204 antibody, clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993).
Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Pig Channel catfish
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor 700 - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Raw 264 cell line
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®700 702 723
- For research purposes only
- 12 months from date of despatch
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or email@example.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
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References for CD204 antibody
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Nature. 364 (6435): 343-6.
de Villiers, W.J. et al. (1994) Macrophage-colony-stimulating factor selectively enhances macrophage scavenger receptor expression and function.
J Exp Med. 180 (2): 705-9.
Hughes, D.A. et al. (1994) Murine M phi scavenger receptor: adhesion function and expression.
Immunol Lett. 43 (1-2): 7-14.
Hughes, D.A. et al. (1995) Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.
Eur J Immunol. 25 (2): 466-73.
Daugherty, A. et al. (2000) Polymorphism of class A scavenger receptors in C57BL/6 mice.
J Lipid Res. 41 (10): 1568-77.
Kaur, H. et al. (2003) Identification of a scavenger receptor homologue on nonspecific cytotoxic cells and evidence for binding to oligodeoxyguanosine.
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Kaur, H. et al. (2004) Single-base oligodeoxyguanosine-binding proteins on nonspecific cytotoxic cells: identification of a new class of pattern-recognition receptors.
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Allred, K.F. et al. (2006) Estrogen receptor-alpha mediates gender differences in atherosclerosis induced by HIV protease inhibitors.
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View The Latest Product References
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Sever-Chroneos, Z. et al. (2011) Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A.
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- Scavenger Receptor Type I/II
- Entrez Gene
- GO Terms
- GO:0005515 protein binding
- GO:0016021 integral to membrane
- GO:0005044 scavenger receptor activity
- GO:0006898 receptor-mediated endocytosis
- GO:0010744 positive regulation of macrophage derived foam cell differentiation
- GO:0010886 positive regulation of cholesterol storage
- GO:0030169 low-density lipoprotein particle binding
- GO:0030301 cholesterol transport
- GO:0034362 low-density lipoprotein particle
- View More GO Terms
- GO:0034381 plasma lipoprotein particle clearance
- GO:0042953 lipoprotein transport
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