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CD204 antibody | 2F8

Rat anti Mouse CD204:Alexa Fluor® 700

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price

Rat anti Mouse CD204 antibody, clone 2F8 recognizes the murine scavenger receptor class A (SR-A), type I and II, also known as CD204. CD204 is expressed by tissue macrophages and functions both as an endocytic receptor for lipoproteins and as an adhesion receptor for macrophages binding to ligand rich tissues e.g. atherosclerotic lesions. Rat anti Mouse CD204 antibody, clone 2F8 inhibits the uptake of acetylated low-density lipoproteins and also inhibits divalent cation independent adhesion (Fraser et al. 1993).

 Rat anti Mouse CD204 antibody, clone 2F8 recognizes an epitope within SRA that is polymorphic in the SRA from C57BL/6 mice. Rat anti Mouse CD204 antibody, clone 2F8 is therefore unsuitable for use with the C57BL/6 mouse strain (Daugherty et al. 2000).

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Channel catfish
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor 700 - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Raw 264 cell line
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised AO rats were fused with cells of the Y3 rat myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®700 702 723
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

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  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
Description Mouse Seroblock FcR

References for CD204 antibody

  1. Fraser, I. et al. (1993) Divalent cation-independent macrophage adhesion inhibited by monoclonal antibody to murine scavenger receptor.
    Nature. 364 (6435): 343-6.
  2. de Villiers, W.J. et al. (1994) Macrophage-colony-stimulating factor selectively enhances macrophage scavenger receptor expression and function.
    J Exp Med. 180 (2): 705-9.
  3. Hughes, D.A. et al. (1994) Murine M phi scavenger receptor: adhesion function and expression.
    Immunol Lett. 43 (1-2): 7-14.
  4. Hughes, D.A. et al. (1995) Murine macrophage scavenger receptor: in vivo expression and function as receptor for macrophage adhesion in lymphoid and non-lymphoid organs.
    Eur J Immunol. 25 (2): 466-73.
  5. Daugherty, A. et al. (2000) Polymorphism of class A scavenger receptors in C57BL/6 mice.
    J Lipid Res. 41 (10): 1568-77.
  6. Kaur, H. et al. (2003) Identification of a scavenger receptor homologue on nonspecific cytotoxic cells and evidence for binding to oligodeoxyguanosine.
    Fish Shellfish Immunol. 15: 169-81.
  7. Kaur, H. et al. (2004) Single-base oligodeoxyguanosine-binding proteins on nonspecific cytotoxic cells: identification of a new class of pattern-recognition receptors.
    Scand J Immunol. 60: 238-48.
  8. Allred, K.F. et al. (2006) Estrogen receptor-alpha mediates gender differences in atherosclerosis induced by HIV protease inhibitors.
    J Biol Chem. 281:1419-25.
  9. View The Latest Product References
  10. Anatelli, F. et al. (2006) Macrophage-targeted photosensitizer conjugate delivered by intratumoral injection.
    Mol Pharm. 3: 654-64.
  11. Luechtenborg, B. et al. (2008) Function of scavenger receptor class A type I/II is not important for smooth muscle foam cell formation.
    Eur J Cell Biol. 87: 91-9.
  12. Nielsen, B.S. et al. (2008) Matrix metalloproteinase 13 is induced in fibroblasts in polyomavirus middle T antigen-driven mammary carcinoma without influencing tumor progression.
    PLoS One. 3 (8): e2959.
  13. Aid, S. et al. (2008) Neuroinflammatory response to lipopolysaccharide is exacerbated in mice genetically deficient in cyclooxygenase-2.
    J Neuroinflammation. 5: 17.
  14. Dewals, B.G. et al. (2010) IL-4Ralpha-independent expression of mannose receptor and Ym1 by macrophages depends on their IL-10 responsiveness.
    PLoS Negl Trop Dis. 4(5):e689.
  15. Moldenhauer, L.M. et al. (2010) GM-CSF is an essential regulator of T cell activation competence in uterine dendritic cells during early pregnancy in mice.
    J Immunol. 185 (11): 7085-96.
  16. Cainarca, S. et al. (2010) A photoprotein in mouse embryonic stem cells measures Ca2+ mobilization in cells and in animals.
    PLoS One. 5: e8882.
  17. Hald, A. et al. (2011) MMP9 is protective against lethal inflammatory mass lesions in the mouse colon.
    Dis Model Mech. 4: 212-27.
  18. Nikolic, D. et al. (2011) SR-A ligand and M-CSF dynamically regulate SR-A expression and function in primary macrophages via p38 MAPK activation.
    BMC Immunol. 12: 37.
  19. Zaynagetdinov, R et al. (2011) A critical role for macrophages in promotion of urethane-induced lung carcinogenesis.
    J Immunol. 187 (11): 5703-11.
  20. Yang, C.N. et al. (2011) Mechanism mediating oligomeric Aβ clearance by naïve primary microglia.
    Neurobiol Dis. 42 (3): 221-30.
  21. Sever-Chroneos, Z. et al. (2011) Surfactant Protein A (SP-A)-mediated Clearance of Staphylococcus aureus Involves Binding of SP-A to the Staphylococcal Adhesin Eap and the Macrophage Receptors SP-A Receptor 210 and Scavenger Receptor Class A.
    J Biol Chem. 286: 4854-70.
  22. Swain, S.D. et al. (2011) Pneumocystis infection in an immunocompetent host can promote collateral sensitization to respiratory antigens.
    Infect Immun. 79 (5): 1905-14.
  23. Horlad, H. et al. (2013) Corosolic acid impairs tumor development and lung metastasis by inhibiting the immunosuppressive activity of myeloid-derived suppressor cells.
    Mol Nutr Food Res. 57 (6): 1046-54.
  24. Verheijden S et al. (2015) Identification of a chronic non-neurodegenerative microglia activation state in a mouse model of peroxisomal β-oxidation deficiency.
    Glia. 63 (9): 1606-20.
  25. Almholt, K. et al. (2015) Spontaneous lung and lymph node metastasis in transgenic breast cancer is independent of the urokinase receptor uPAR.
    Clin Exp Metastasis. 32 (6): 543-54.
  26. Tao, J. et al. (2015) ClC-3 deficiency prevents atherosclerotic lesion development in ApoE-/- mice.
    J Mol Cell Cardiol. 87: 237-247.
  27. Koronyo Y et al. (2015) Therapeutic effects of glatiramer acetate and grafted CD115+ monocytes in a mouse model of Alzheimer's disease.
    Brain. 138 (Pt 8): 2399-422.
  28. Prins, J.R. et al. (2015) Unstable Foxp3+ regulatory T cells and altered dendritic cells are associated with lipopolysaccharide-induced fetal loss in pregnant interleukin 10-deficient mice.
    Biol Reprod. 93 (4): 95.
  29. Kokubu, Y. et al. (2016) Induction of protumoral CD11c(high) macrophages by glioma cancer stem cells through GM-CSF.
    Genes Cells. 21 (3): 241-51.
  30. Fujiwara, Y. et al. (2016) Onionin A, a sulfur-containing compound isolated from onions, impairs tumor development and lung metastasis by inhibiting the protumoral and immunosuppressive functions of myeloid cells.
    Mol Nutr Food Res. 60 (11): 2467-80.
  31. Sapkota, M. et al. (2016) Malondialdehyde-Acetaldehyde-Adducted Surfactant Protein Alters Macrophage Functions Through Scavenger Receptor A.
    Alcohol Clin Exp Res. 40 (12): 2563-2572.
  32. Tsay, H.J. et al. (2016) Identifying N-linked glycan moiety and motifs in the cysteine-rich domain critical for N-glycosylation and intracellular trafficking of SR-AI and MARCO.
    J Biomed Sci. 23: 27.
  33. Haupt, F. et al. (2019) Retinal myeloid cells regulate tip cell selection and vascular branching morphogenesis via Notch ligand Delta-like 1.
    Sci Rep. 9 (1): 9798.
  34. Yamamoto, M. et al. (2019) Evaluation of neurobehavioral impairment in methylmercury-treated KK-Ay mice by dynamic weight-bearing test.
    J Appl Toxicol. 39 (2): 221-30.
  35. Shiau, D.J. et al. (2020) Hepatocellular carcinoma-derived high mobility group box 1 triggers M2 macrophage polarization via a TLR2/NOX2/autophagy axis.
    Sci Rep. 10 (1): 13582.
  36. Tian, L.X. et al. (2020) Cytochrome P450 1A1 enhances inflammatory responses and impedes phagocytosis of bacteria in macrophages during sepsis.
    Cell Commun Signal. 18 (1): 70.
  37. Li, S. et al. (2020) Activated Bone Marrow-Derived Macrophages Eradicate Alzheimer's-Related Aβ(42) Oligomers and Protect Synapses.
    Front Immunol. 11: 49.
  38. Wang, Y. et al. (2021) Scavenger receptor A1 participates in uptake of Leptospira interrogans serovar Autumnalis strain 56606v and inflammation in mouse macrophages.
    Emerg Microbes Infect. : 1-39.
  39. Mañucat-Tan, N. et al. (2021) Hypochlorite-induced aggregation of fibrinogen underlies a novel antioxidant role in blood plasma.
    Redox Biol. 40: 101847.
  40. Jia, J. et al. (2022) Ferritin triggers neutrophil extracellular trap-mediated cytokine storm through Msr1 contributing to adult-onset Still's disease pathogenesis.
    Nat Commun. 13 (1): 6804.

Further Reading

  1. Gordon, S. (1995) The macrophage.
    Bioessays. 17 (11): 977-86.

Blocking assay

Flow Cytometry

Functional Assays

Immunohistology - Frozen

Immunohistology - Paraffin

Western Blotting

Scavenger Receptor Type I/II
Entrez Gene
GO Terms
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0005044 scavenger receptor activity
GO:0006898 receptor-mediated endocytosis
GO:0010744 positive regulation of macrophage derived foam cell differentiation
GO:0010886 positive regulation of cholesterol storage
GO:0030169 low-density lipoprotein particle binding
GO:0030301 cholesterol transport
GO:0034362 low-density lipoprotein particle
GO:0034381 plasma lipoprotein particle clearance
GO:0042953 lipoprotein transport



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