CD19 antibody | 6D5

Rat anti Mouse CD19:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
6D5
Isotype
IgG2a
Specificity
CD19

Product Code Applications Pack Size List Price Your Price Qty
MCA1439A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Rat anti Mouse CD19 antibody, clone 6D5 recognizes the murine CD19 cell surface antigen, a ~95 kDa glycoprotein expressed by B lymphocytes. Rat anti Mouse CD19 antibody, clone 6D5 recognizes the same, or a closely related epitope as clone Rat anti Mouse CD19 antibody, clone ID3 in cross-competition assays.

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Human K562 cell line transfected with murine CD19.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:Alexa Fluor® 647 MCA1212A647 F 100 Tests/1ml loader
List Price Your Price
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Description Rat IgG2a Negative Control:Alexa Fluor® 647

References for CD19 antibody

  1. Vernooy, J.H. et al. (2002) Long-term intratracheal lipopolysaccharide exposure in mice results in chronic lung inflammation and persistent pathology.
    Am J Respir Cell Mol Biol. 26 (1): 152-9.
  2. Andrew, D. and Aspinall. R. (2001) Il-7 and not stem cell factor reverses both the increase in apoptosis and the decline in thymopoiesis seen in aged mice.
    J Immunol. 166: 1524-30.
  3. Bermudez-Fajardo, A. et al. (2011) The effect of Chlamydophila pneumoniae Major Outer Membrane Protein (MOMP) on macrophage and T cell-mediated immune responses.
    Immunobiology. 216: 152-63.
  4. De Jesus, M. et al. (2009) Galactoxylomannan-mediated immunological paralysis results from specific B cell depletion in the context of widespread immune system damage.
    J Immunol. 183: 3885-94.
  5. Jégou, J.F. et al. (2007) C3d binding to the myelin oligodendrocyte glycoprotein results in an exacerbated experimental autoimmune encephalomyelitis.
    J Immunol. 178: 3323-31.
  6. Starck, J. et al. (2010) Inducible Fli-1 gene deletion in adult mice modifies several myeloid lineage commitment decisions and accelerates proliferation arrest and terminal erythrocytic differentiation.
    Blood. 116: 4795-805.
  7. Scotland, R.S. et al. (2011) Sex differences in resident immune cell phenotype underlie more efficient acute inflammatory responses in female mice.
    Blood. 118 (22): 5918-27.
  8. White, H.N. and Meng, Q.H. (2012) Recruitment of a Distinct but Related Set of VH Sequences into the Murine CD21hi/CD23- Marginal Zone B Cell Repertoire to That Seen in the Class-Switched Antibody Response.
    J Immunol. 188: 287-93.
  9. View The Latest Product References
  10. Reynaud, J.M. et al. (2014) Human herpesvirus 6A infection in CD46 transgenic mice: viral persistence in the brain and increased production of proinflammatory chemokines via Toll-like receptor 9.
    J Virol. 88: 5421-36.
  11. Candolfi, M. et al. (2011) B cells are critical to T-cell-mediated antitumor immunity induced by a combined immune-stimulatory/conditionally cytotoxic therapy for glioblastoma.
    Neoplasia. 13: 947-60.
  12. Takabayashi, H. et al. (2014) Anti-inflammatory activity of bone morphogenetic protein signaling pathways in stomachs of mice.
    Gastroenterology. 147: 396-406.e7.
  13. Weiss-Gayet, M. et al. (2016) Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors.
    PLoS One. 11 (4): e0153860.
  14. Meng, Q.H. & White, H.N. (2017) CD21int CD23+ follicular B cells express antigen-specific secretory IgM mRNA as primary and memory responses.
    Immunology. 151 (2): 211-8.
  15. Mccubbrey, A.L. et al. (2016) MicroRNA-34a Negatively Regulates Efferocytosis by Tissue Macrophages in Part via SIRT1.
    J Immunol. 196 (3): 1366-75.
  16. Vila-Caballer, M. et al. (2019) Disruption of the CCL1-CCR8 axis inhibits vascular Treg recruitment and function and promotes atherosclerosis in mice.
    J Mol Cell Cardiol. 132: 154-63.

Flow Cytometry

Immunofluorescence

RRID
AB_324762
UniProt
P25918
Entrez Gene
Cd19
GO Terms
GO:0016021 integral to membrane
GO:0009897 external side of plasma membrane
GO:0050853 B cell receptor signaling pathway
View more products with CD19 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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