CD180 antibody | RP/14

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Rat anti Mouse CD180

Product Type
Monoclonal Antibody
Clone
RP/14
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2149 F* FN* IP WB 0.25 mg
Rat anti Mouse CD180 antibody, clone RP/14  recognizes mouse CD180, also known as Lymphocyte antigen 78 or Radioprotective 105 kDa protein encoded by the RP105 gene. Mouse CD180 is a 661 amino acid ~105 kDa single pass type 1 transmembrane glycoprotein, containing multiple leucine rich repeats within LRRCT and LRRNT domains. CD180 belongs to the toll-like receptor family and is primarily expressed in mature peripheral B cells and is thought to play a role in the innate immune response to bacterial lipopolysaccharide, through co-operation with MD-1 and TLR4 (UniProt: Q62192).

Cross linking of CD180 with clone RP/14 has been reported to induce resistance against irradiation induced apoptosis, B-cell proliferation and up-regulation of a co-stimulatory molecule B7.2 (Miyake et al. 1998).

Product Details

Target Species
Mouse
Product Form
Purified IgG - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q62192 Related reagents
Entrez Gene
Cd180 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0006954 inflammatory response
GO:0045087 innate immune response
Regulatory
For research purposes only

Applications of CD180 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
Functional Assays 2
Immunoprecipitation
Western Blotting
  1. 1 CD180 is expressed at the cell surface in association with MD-1. CD180 may also be found free within the cell. For detection of intracellular CD180, membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm™ (Product Code BUF09) for this purpose.
  2. 2 This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays. Bio-Rad recommend the use of EQU003 for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Rat IgG:Alk. Phos. (Mouse Adsorbed) STAR131A 1 ml C E P WB
Goat anti Rat IgG:Biotin (Mouse Adsorbed) STAR131B 0.5 mg C E IF P WB
Rabbit F(ab')2 anti Rat IgG:Dylight®800 STAR16D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Rat IgG:FITC STAR17B 1 mg F
Rabbit F(ab')2 anti Rat IgG:HRP STAR21B 1 mg C E P RE
Goat F(ab')2 anti Rat IgG:FITC (Mouse Adsorbed) STAR69 0.5 ml F
Goat anti Rat IgG:DyLight®549 (Mouse Adsorbed) STAR71D549GA 0.1 mg F IF
Goat anti Rat IgG:DyLight®649 (Mouse Adsorbed) STAR71D649GA 0.1 mg F IF
Goat anti Rat IgG:Dylight®800 (Mouse Adsorbed) STAR71D800GA 0.1 mg F IF WB
Goat anti Rat IgG:HRP (Mouse Adsorbed) STAR72 0.5 mg C E P
Goat F(ab')2 anti Rat IgG:RPE (Mouse Adsorbed) STAR73 0.5 ml F

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control MCA1212 1 ml E F

Product Specific References

References for CD180 antibody

  1. Nagai, Y. et al. (2002) Requirement for MD-1 in cell surface expression of RP105/CD180 and B-cell responsiveness to lipopolysaccharide.
    Blood. 99 (5): 1699-705.
  2. Miyake, K. et al. (1998) Mouse MD-1, a molecule that is physically associated with RP105 and positively regulates its expression.
    J Immunol. 161 (3): 1348-53.
  3. Geraldes, P. et al. (2007) Ig heavy chain promotes mature B cell survival in the absence of light chain.
    J Immunol. 179: 1659-68