CD161 / NK1.1 antibody | PK136
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|Mouse anti Mouse CD161 / NK1.1 antibody, clone PK136 recognizes the mouse NK1.1 cell surface antigen, a cell surface glycoprotein encoded by members of the NKR-P1 gene family. The NK1.1 surface antigen is also known as CD161b/CD161c and Ly-55.
In the mouse the NKR-P1 family has three members, NKR-P1A, -B and -C, whilst in the human only one member has been identified. The human protein has received the designation CD161, and the mouse proteins have been referred to as CD161a, -b, -c etc.
Although previously thought to recognize only CD161c, recent data has shown that the PK136 antibody may also react with CD161b. CD161c expression itself is strain specific in mice, but recognition of CD161b by PK136 appears to be even more complex, as only some CD161b positive strains are labelled by the antibody. Engagement of CD161c has been reported to have activating function in NK cells, whilst engagement of CD161b is inhibitory.
Mouse anti Mouse NK1.1 Antigen antibody, clone PK136 is useful for the identification of NK cells in selected strains of mice (positive on C57BL, FVB/N and NZB, but negative on AKR and BALB/c) and is also expressed by rare subsets of T cells and monocytes. Mouse anti Mouse NK1.1 antibody, clone PK136 has also been used for in vivo depletion of NK cells and in vitro activation of NK cells.
- Target Species
- Species Cross-Reactivity
Target Species Cross Reactivity Rat Human
- N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to StarBright Violet 440 - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
- Spleen and bone marrow cells from CE mice.
- Fusion Partners
- Spleen cells from immunised (C3H x BALB/c) FI Hybrid were fused with cells of the Sp2/0 - Ag14 myeloma cell line.
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) StarBright Violet 440 385 438
- For research purposes only
- 12 months from date of despatch
- This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts
This product should be stored undiluted.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.
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Koo, G.C. & Peppard, J.R. (1984) Establishment of monoclonal anti-Nk-1.1 antibody.
Hybridoma. 3 (3): 301-3.
References for CD161 / NK1.1 antibody
Kung, S.K. & Miller RG (1995) The NK1.1 antigen in NK-mediated F1 antiparent killing in vitro.
J Immunol. 154 (4): 1624-33.
Wang, M. et al. (1998) Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen-stimulated cytokine and antibody responses.
J Immunol. 160 (3): 1098-105.
Koo, G.C. et al. (1986) The NK-1.1(-) mouse: a model to study differentiation of murine NK cells.
J Immunol. 137 (12): 3742-7.
Kung, S.K. et al. (1999) The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells.
J Immunol. 162 (10): 5876-87.
Carlyle, J.R. et al. (1999) Mouse NKR-P1B, a novel NK1.1 antigen with inhibitory function.
J Immunol. 162 (10): 5917-23.
Carnemolla, B. et al. (2002) Enhancement of the antitumor properties of interleukin-2 by its targeted delivery to the tumor blood vessel extracellular matrix.
Blood. 99: 1659-65.
Carpentier, A.F. et al. (1999) Oligodeoxynucleotides containing CpG motifs can induce rejection of a neuroblastoma in mice.
Cancer Res. 59: 5429-32.
Sakai, T. et al. (2010) Inflammatory disease and cancer with a decrease in Kupffer cell numbers in Nucling-knockout mice.
Int J Cancer. 126: 1079-94.
View The Latest Product References
Svensson, L. et al. (2003) gammadelta T cells contribute to the systemic immunoglobulin E response and local B-cell reactivity in allergic eosinophilic airway inflammation.
Immunology. 108 (1): 98-108.
Hazlett, L.D. et al. (2007) NKT cells are critical to initiate an inflammatory response after Pseudomonas aeruginosa ocular infection in susceptible mice.
J Immunol. 179 : 1138-46.
Joseph-Pietras, D. et al. (2006) Anti-tumoural activity of peripheral blood mononuclear cells against melanoma cells: discrepant in-vitro and in-vivo effects.
Melanoma Res. 16: 325-33.
Gock, H. et al. (2014) Altered glycosylation in donor mice causes rejection of strain-matched skin and heart grafts.
Am J Transplant. 14 (4): 797-805.
Khallouf, H. et al. (2012) 5-Fluorouracil and interferon-α immunochemotherapy enhances immunogenicity of murine pancreatic cancer through upregulation of NKG2D ligands and MHC class I.
J Immunother. 35 (3): 245-53.
Ebbinghaus, C. et al. (2005) Engineered vascular-targeting antibody-interferon-gamma fusion protein for cancer therapy.
Int J Cancer. 116 (2): 304-13.
Ekstrand-Hammarström, B. et al. (2011) Inhalation of alkylating mustard causes long-term T cell-dependent inflammation in airways and growth of connective tissue.
Toxicology. 280 (3): 88-97.
Klezovich-Bénard M et al. (2012) Mechanisms of NK cell-macrophage Bacillus anthracis crosstalk: a balance between stimulation by spores and differential disruption by toxins.
PLoS Pathog. 8 (1): e1002481.
Halin, C. et al. (2002) Enhancement of the antitumor activity of interleukin-12 by targeted delivery to neovasculature.
Nat Biotechnol. 20 (3): 264-9.
Gustafsson, Å. et al. (2015) Differential cellular responses in healthy mice and in mice with established airway inflammation when exposed to hematite nanoparticles.
Toxicol Appl Pharmacol. 288 (1): 1-11.
Flavell, D.J. et al. (2019) The TLR3 Agonist Poly Inosinic:Cytidylic Acid Significantly Augments the Therapeutic Activity of an Anti-CD7 Immunotoxin for Human T-cell Leukaemia.
Biomedicines. 7 (1) Feb 16 [Epub ahead of print].
Miao, M. et al. (2021) Reevaluation of NOD/SCID Mice as NK Cell-Deficient Models.
Biomed Res Int. 2021: 8851986.
Arase, N. et al. (1997) Association with FcRgamma is essential for activation signal through NKR-P1 (CD161) in natural killer (NK) cells and NK1.1+ T cells.
J Exp Med. 186 (12): 1957-63.
- Entrez Gene
- GO Terms
- GO:0004872 receptor activity
- GO:0005887 integral to plasma membrane
- GO:0005529 sugar binding
- GO:0009897 external side of plasma membrane
- GO:0042803 protein homodimerization activity
- GO:0045953 negative regulation of natural killer cell mediated cytotoxicity
- GO:0045954 positive regulation of natural killer cell mediated cytotoxicity
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