CD16/CD32 antibody | FCR4G8

Rat anti Mouse CD16/CD32:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
FCR4G8
Isotype
IgG2b
Specificity
CD16/CD32

Product Code Applications Pack Size List Price Your Price Qty
MCA2305A647
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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MCA2305A647T
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SDS Safety Datasheet SDS
F 25 Tests/0.25ml loader
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Rat anti Mouse CD16/CD32 antibody, clone FCR4G8 recognizes an epitope expressed by the murine low affinity Fc receptors, Fc gamma III (CD16) and Fc gamma II (CD32). In the mouse only the transmembrane form of CD16 is reported to exist, which is expressed on macrophages, NK cells, granulocytes, myeloid precursors, and subpopulations of T lymphocytes. CD32 is primarily expressed on cells of the myeloid lineage and also on mature B lymphocytes.

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
PU5 1.8 IOE7 Balb/c mouse cell line.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Cells from immunised rats were fused with cells of the mouse P3X63-Ag8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

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References for CD16/CD32 antibody

  1. da Silva, R.G. et al. (2010) Endothelial alpha3beta1-integrin represses pathological angiogenesis and sustains endothelial-VEGF.
    Am J Pathol. 177 (3): 1534-48.
  2. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
    J Immunol. 186: 3441-51.
  3. Jones, D.T. et al. (2013) Endogenous ribosomal protein L29 (RPL29): a newly identified regulator of angiogenesis in mice.
    Dis Model Mech. 6: 115-24.
  4. Kapellos, T.S. et al. (2016) A Novel Real Time Imaging Platform to Quantify Macrophage Phagocytosis.
    Biochem Pharmacol. Jul 27. pii: S0006-2952(16)30176-9. [Epub ahead of print]
  5. Lunnon, K. et al. (2011) Systemic inflammation modulates Fc receptor expression on microglia during chronic neurodegeneration.
    J Immunol. 186 (12): 7215-24.
  6. Teeling, J.L. et al. (2012) Intracerebral immune complex formation induces inflammation in the brain that depends on Fc receptor interaction.
    Acta Neuropathol. 124 (4): 479-90.
  7. Murinello, S. et al. (2014) Fcγ receptor upregulation is associated with immune complex inflammation in the mouse retina and early age-related macular degeneration.
    Invest Ophthalmol Vis Sci. 55 (1): 247-58.
  8. Hart, A.D. et al. (2012) Age related changes in microglial phenotype vary between CNS regions: grey versus white matter differences.
    Brain Behav Immun. 26 (5): 754-65.
  9. View The Latest Product References
  10. Birjandi, S.Z. et al. (2011) Alterations in marginal zone macrophages and marginal zone B cells in old mice.
    J Immunol. 186 (6): 3441-51.
  11. Hart, R. & Greaves, D.R. (2010) Chemerin contributes to inflammation by promoting macrophage adhesion to VCAM-1 and fibronectin through clustering of VLA-4 and VLA-5.
    J Immunol. 185 (6): 3728-39.
  12. Ahmed, N. et al. (2022) Blood stem cell PU.1 upregulation is a consequence of differentiation without fast autoregulation.
    J Exp Med. 219 (1): e20202490.

Synonyms
Mouse Seroblock FcR
RRID
AB_566550
UniProt
P08508
P08101
Entrez Gene
Fcgr2b
Fcgr3
GO Terms
GO:0005886 plasma membrane
GO:0001788 antibody-dependent cellular cytotoxicity
GO:0001798 positive regulation of type IIa hypersensitivity
GO:0001805 positive regulation of type III hypersensitivity
GO:0001812 positive regulation of type I hypersensitivity
GO:0001820 serotonin secretion
GO:0016021 integral to membrane
GO:0006910 phagocytosis, recognition
GO:0006911 phagocytosis, engulfment
GO:0007166 cell surface receptor linked signaling pathway
GO:0019770 IgG receptor activity
GO:0030593 neutrophil chemotaxis
GO:0042535 positive regulation of tumor necrosis factor biosynthetic process
GO:0042590 antigen processing and presentation of exogenous peptide antigen via MHC class I
GO:0045576 mast cell activation
GO:0050766 positive regulation of phagocytosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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