CD14 antibody | biG53

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Mouse anti Mouse CD14:RPE

Product Type
Monoclonal Antibody
Clone
biG53
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2745PE F 100 Tests
Mouse anti Mouse CD14 antibody, clone biG53 recognizes mouse CD14, a ~55 kDa a glycophosphoinositol-linked cell surface protein that also exists in a soluble form. Expression of CD14 is restricted to the myeloid lineage and is predominantly expressed on peripheral blood monocytes, weakly expressed on granulocytes and absent on myeloid progenitors. The CD14 molecule acts as a pattern recognition receptor, which binds lipopolysaccharide (LPS) and other microbial products such as lipoteichoic acid, lipoproteins and peptidoglycan. Binding of LPS to CD14 mediates the innate immune response to LPS and requires the Toll-like receptor-4 (TLR4)/MD-2 complex for effective signalling.

Mouse anti Mouse CD14 antibody, clone biG53 inhibits the binding of CD14.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Recombinant mouse CD14.

Storage Information

Storage
Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of reconstitution.

More Information

UniProt
P10810 Related reagents
Entrez Gene
Cd14 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0006954 inflammatory response
GO:0031225 anchored to membrane
GO:0045087 innate immune response
GO:0045121 membrane raft
Regulatory
For research purposes only

Applications of CD14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F