CD122 antibody | TM-BETA 1

Rat anti Mouse CD122:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
TM-BETA 1
Isotype
IgG2b
Specificity
CD122

Product Code Applications Pack Size List Price Your Price Qty
MCA2363A647
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price
loader
Search for Batch Specific Datasheets

Rat anti Mouse CD122 antibody, clone TM-BETA 1 recognizes the murine CD122 cell surface glycoprotein, a ~75-85 kDa molecule also known as interleukin-2 receptor (IL-2R) beta chain. In mouse, CD122 is primarily expressed on natural killer (NK) cells and at lower levels on lymphocyte subsets, monocytes and macrophages.

CD122 can associate with the IL-2R alpha chain (CD25) and CD132 to form a functional high affinity receptor complex that binds IL-2. CD132/CD122 heterodimers can also be formed which bind IL-2 with intermediate affinity. IL-2 binding to the IL-2R is important in the generation and differentiation of T lymphocytes and NK cells.

Rat anti Mouse CD122 antibody, clone TM-BETA 1 blocks IL-2 high affinity binding to the IL-2R beta receptor (Tanaka et al. 1991).

Target Species
Mouse
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Cells from murine IL-2RB+ transformant TART-mBeta.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Lymph node cells from immunised Sprague Dawley rats were fused with cells of the mouse PA1 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system to a concentration equivalent to their test reagent.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

References for CD122 antibody

  1. Tanaka, T. et al. (1991) A novel monoclonal antibody against murine IL-2 receptor beta-chain. Characterization of receptor expression in normal lymphoid cells and EL-4 cells.
    J Immunol. 147 (7): 2222-8.
  2. Leung, K.T. (2011) The tetraspanin CD9 regulates migration, adhesion, and homing of human cord blood CD34+ hematopoietic stem and progenitor cells.
    Blood. 117: 1840-50.
  3. Chenoweth, M.J. et al. (2012) IL-15 Can Signal via IL-15Rα, JNK, and NF-κB To Drive RANTES Production by Myeloid Cells.
    J Immunol. 188: 4149-57.
  4. Mertens, J. et al. (2009) Streptococcus pneumoniae serotype 1 capsular polysaccharide induces CD8CD28 regulatory T lymphocytes by TCR crosslinking.
    PLoS Pathog. 5 (9): e1000596.

Synonyms
Interleukin-2 Receptor Beta Chain
RRID
AB_566483
UniProt
P16297
Entrez Gene
Il2rb
GO Terms
GO:0016021 integral to membrane
GO:0004896 cytokine receptor activity
GO:0009897 external side of plasma membrane
GO:0019976 interleukin-2 binding
View more products with CD122 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Mouse Products