CD122 antibody | TM-BETA 1
Rat anti Mouse CD122 antibody, clone TM-BETA 1 (MCA2363) used to evaluate IL-2R expression on mouse lymphocytes
Image caption:
Phenotype of Sp1-induced CD8+CD28− T lymphocytes in vivo.
C57BL/6 WT mice were challenged intraperitoneally with Sp1, mSp1 (100 μg/mouse, respectively), or SCCA alone. After 18 h, spleen cells were isolated. Cells were stained for CD8 and CD28 surface expression and prepared for flow cytometry. Flow cytometry histograms in A–C are representative for one out of five experiments with 4 mice each. A. Intraperitoneal Sp1 application induces CD8+CD28− T lymphocytes in vivo. The percentages ±SD (n = 4) of the respective CD8+CD28− cells are given above the marker. B. For determination of the phenotype of the Sp1-induced CD8+CD28− T lymphocytes in C57BL/6 mice, CD8+CD28− and CD8+CD28+ T cells were stained with antibodies specific for surface markers and prepared for flow cytometry analysis. Percentages of positive cells±SD (n = 4) for the CD8+CD28− and CD8+CD28+ T cell subsets are displayed above the marker. C. C57BL/6 WT mice were challenged intraperitoneally with Sp1, mSp1 (100 μg/mouse, respectively), or SCC alone. After 18 h, spleen cells were isolated. CD8+CD28− cells were analyzed for LAP surface expression and intracellular cytokines by flow cytometry. Percentages of positive cells ±SD (n = 4) are given below the marker.
From: Citation: Mertens J, Fabri M, Zingarelli A, Kubacki T, Meemboor S, Groneck L, et al. (2009)
Streptococcus pneumoniae Serotype 1 Capsular Polysaccharide Induces CD8+CD28− Regulatory T Lymphocytes by TCR Crosslinking.
PLoS Pathog 5(9): e1000596.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Filter by Application:
F ResetRat anti Mouse CD122:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- TM-BETA 1
- Isotype
- IgG2b
- Specificity
- CD122
Antibody Quality and Performance Guarantee
| Rat anti Mouse CD122 antibody, clone TM-BETA 1 recognizes the murine CD122 cell surface glycoprotein, a ~75-85 kDa molecule also known as interleukin-2 receptor (IL-2R) beta chain. In mouse, CD122 is primarily expressed on natural killer (NK) cells and at lower levels on lymphocyte subsets, monocytes and macrophages. CD122 can associate with the IL-2R alpha chain (CD25) and CD132 to form a functional high affinity receptor complex that binds IL-2. CD132/CD122 heterodimers can also be formed which bind IL-2 with intermediate affinity. IL-2 binding to the IL-2R is important in the generation and differentiation of T lymphocytes and NK cells. Rat anti Mouse CD122 antibody, clone TM-BETA 1 blocks IL-2 high affinity binding to the IL-2R beta receptor (Tanaka et al. 1991). |
- Target Species
- Mouse
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647- liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Cells from murine IL-2RB+ transformant TART-mBeta.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Lymph node cells from immunised Sprague Dawley rats were fused with cells of the mouse PA1 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for CD122 antibody
-
Tanaka, T. et al. (1991) A novel monoclonal antibody against murine IL-2 receptor beta-chain. Characterization of receptor expression in normal lymphoid cells and EL-4 cells.
J Immunol. 147 (7): 2222-8. -
Leung, K.T.
(2011) The tetraspanin CD9 regulates migration, adhesion, and homing of human cord blood CD34+ hematopoietic stem and progenitor cells.
Blood. 117: 1840-50. -
Chenoweth, M.J. et al. (2012) IL-15 Can Signal via IL-15Rα, JNK, and NF-κB To Drive RANTES Production by Myeloid Cells.
J Immunol. 188: 4149-57. -
Mertens, J. et al. (2009) Streptococcus pneumoniae serotype 1 capsular polysaccharide induces CD8CD28 regulatory T lymphocytes by TCR crosslinking.
PLoS Pathog. 5 (9): e1000596.
- Synonyms
- Interleukin-2 Receptor Beta Chain
- RRID
- AB_566483
- UniProt
- P16297
- Entrez Gene
- Il2rb
- GO Terms
- GO:0016021 integral to membrane
- GO:0004896 cytokine receptor activity
- GO:0009897 external side of plasma membrane
- GO:0019976 interleukin-2 binding
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