CD120a antibody | HM104

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Rat anti Mouse CD120a:RPE

Product Type
Monoclonal Antibody
Clone
HM104
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2078PET F 25 Tests
MCA2078PE F 100 Tests
Rat anti Mouse CD120a antibody, clone HM104 recognizes an extracellular region of mouse CD120a, also known as TNF receptor type I (TNFR1). TNFR1 is an ubiquitously expressed transmembrane glycoprotein and member of the tumor necrosis factor receptor (TNFR) superfamily, which plays a key role in regulating the inflammatory response, acting as a receptor for tumor necrosis factor alpha (TNF-alpha) and lymphotoxin-alpha (TNF-beta).

CD120a also plays a role in apoptosis, cytokine induction, cell differentiation, tumor necrosis and early stages of graft-versus-host disease, and its soluble form sTNFR1, acts as an inhibitor of TNF-alpha induced responses by acting as a decoy receptor.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02%Sodium Azide
0.5%Bovine Serum Albumin

Storage Information

Storage
Store at +4oC.

DO NOT FREEZE.

This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
6 months from date of despatch.

More Information

UniProt
P25118 Related reagents
Entrez Gene
Tnfrsf1a Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0042742 defense response to bacterium
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005031 tumor necrosis factor receptor activity
GO:0006693 prostaglandin metabolic process
GO:0006954 inflammatory response
GO:0045121 membrane raft
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050729 positive regulation of inflammatory response
Regulatory
For research purposes only

Applications of CD120a antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/B).

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

References for CD120a antibody

  1. Pearl-Yafe, M. et al. (2010) Tumor necrosis factor receptors support murine hematopoietic progenitor function in the early stages of engraftment.
    Stem Cells. 28 (7): 1270-80.
  2. Nakagawa, J. et al. (2007) TNF expressed by tumor-associated macrophages, but not microglia, can eliminate glioma.
    Int J Oncol. 30 (4): 803-11.