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CD120a antibody | HM104

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Rat anti Mouse CD120a:RPE

Product Type
Monoclonal Antibody
Clone
HM104
Isotype
IgG2a
Specificity
CD120a

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Technical Suppport
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MCA2078PE
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MCA2078PET
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Rat anti Mouse CD120a antibody, clone HM104 recognizes an extracellular region of mouse CD120a, also known as TNF receptor type I (TNFR1). TNFR1 is an ubiquitously expressed transmembrane glycoprotein and member of the tumor necrosis factor receptor (TNFR) superfamily, which plays a key role in regulating the inflammatory response, acting as a receptor for tumor necrosis factor alpha (TNF-alpha) and lymphotoxin-alpha (TNF-beta).

CD120a also plays a role in apoptosis, cytokine induction, cell differentiation, tumor necrosis and early stages of graft-versus-host disease, and its soluble form sTNFR1, acts as an inhibitor of TNF-alpha induced responses by acting as a decoy receptor.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
Pack Size: 100 Tests
<0.1% sodium azide (NaN3)
0.2% bovine serum albumin
Pack Size: 25 Tests
<0.1% sodium azide (NaN3)
0.5% bovine serum albumin
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
6 months from date of despatch

Store at +4°C.
DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl.
The Fc region of monoclonal antibodies may bind to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR (BUF041A/BUF041B).

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests loader
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Description Rat IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

References for CD120a antibody

  1. Pearl-Yafe, M. et al. (2010) Tumor necrosis factor receptors support murine hematopoietic progenitor function in the early stages of engraftment.
    Stem Cells. 28 (7): 1270-80.
  2. Nakagawa, J. et al. (2007) TNF expressed by tumor-associated macrophages, but not microglia, can eliminate glioma.
    Int J Oncol. 30 (4): 803-11.

Synonyms
TNF Receptor Type I
RRID
AB_323589
UniProt
P25118
Entrez Gene
Tnfrsf1a
GO Terms
GO:0005886 plasma membrane
GO:0042742 defense response to bacterium
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005031 tumor necrosis factor receptor activity
GO:0006693 prostaglandin metabolic process
GO:0006954 inflammatory response
GO:0045121 membrane raft
GO:0045944 positive regulation of transcription from RNA polymerase II promoter
GO:0050729 positive regulation of inflammatory response
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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