CD11b antibody | M1/70.15

Rat anti Mouse CD11b:Alexa Fluor® 647

Product Type
Monoclonal Antibody
Clone
M1/70.15
Isotype
IgG2b
Specificity
CD11b

Product Code Applications Pack Size List Price Your Price Qty
MCA74A647
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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MCA74A647T
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F 25 Tests/0.25ml loader
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Search for Batch Specific Datasheets

Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages.

The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages.

Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982).

Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995).

This product is routinely tested in flow cytometry on mouse peritoneal macrophages.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rabbit
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Alexa Fluor® 647- liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
T cell enriched splenocytes from B10 mice.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically via the Fc region to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR

Source Reference

  1. Springer, T. et al. (1978) Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.
    Eur J Immunol. 8 (8): 539-51.

Antibody Characterization Reference

  1. Springer, T. et al. (1979) Mac-1: a macrophage differentiation antigen identified by monoclonal antibody.
    Eur J Immunol. 9 (4): 301-6. Eur J Immunol. 9 (4): 301-6.

References for CD11b antibody

  1. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  2. Lebson, L. et al. (2010) Trafficking CD11b-positive blood cells deliver therapeutic genes to the brain of amyloid-depositing transgenic mice.
    J Neurosci. 30: 9651-8.
  3. Lefevre, L. et al. (2010) PPARγ ligands switched high fat diet-induced macrophage M2b polarization toward M2a thereby improving intestinal Candida elimination.
    PLoS One. 5: e12828.
  4. Patel, P.C. and Harrison, R.E. (2008) Membrane ruffles capture C3bi-opsonized particles in activated macrophages.
    Mol Biol Cell. 19: 4628-39.
  5. Zhang, H. et al. (2010) A p38 mitogen-activated protein kinase-dependent mechanism of disinhibition in spinal synaptic transmission induced by tumor necrosis factor-alpha.
    J Neurosci. 30: 12844-55.
  6. Cunningham, K. et al. (2011) Conditional deletion of Ccm2 causes hemorrhage in the adult brain: a mouse model of human cerebral cavernous malformations.
    Hum Mol Genet. 20: 3198-206.
  7. Khan, A.A. et al. (2015) Therapeutic Immunization with a Mixture of Herpes Simplex Virus Type 1 Glycoprotein D Derived "Asymptomatic" Human CD8+ T-cell Epitopes Decreases Spontaneous Ocular Shedding in Latently Infected HLA Transgenic Rabbits: Association with Low Frequency of Local PD-1+TIM-3+CD8+ Exhausted T Cells.
    J Virol. pii: JVI.00788-15.
  8. Beller, D.I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.
    J Exp Med. 156 (4): 1000-9.
  9. View The Latest Product References
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  13. Redensek, A. et al. (2011) Expression and detrimental role of hematopoietic prostaglandin D synthase in spinal cord contusion injury.
    Glia. 59: 603-14.
  14. Brochard, V. et al (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
    J Clin Invest. 119: 182-92.
  15. Chinnery, H.R. et al. (2010) Novel characterization of monocyte-derived cell populations in the meninges and choroid plexus and their rates of replenishment in bone marrow chimeric mice.
    J Neuropathol Exp Neurol. 69: 896-909.
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  17. Gales, A. et al (2010) PPARgamma controls dectin-1 expression required for host antifungal defense against Candida albicans.
    PLoS Pathog. 6 : e1000714.
  18. Geier, H. and Celli, J. (2011) Phagocytic Receptors Dictate Phagosomal Escape and Intracellular Proliferation of Francisella tularensis.
    Infect Immun. 79: 2204-14.
  19. Ghasemlou, N. et al. (2010) Mitogen-activated protein kinase-activated protein kinase 2 (MK2) contributes to secondary damage after spinal cord injury.
    J Neurosci. 30: 13750-9.
  20. Huang, Q.Q. et al (2008) Role of H2-calponin in regulating macrophage motility and phagocytosis.
    J Biol Chem. 283: 25887-99.
  21. Hudcovic, T. et al (2009) Monocolonization with Bacteroides ovatus protects immunodeficient SCID mice from mortality in chronic intestinal inflammation caused by long-lasting dextran sodium sulfate treatment.
    Physiol Res. 58: 101-10.
  22. Kanu, N. et al. (2010) The ATM cofactor ATMIN protects against oxidative stress and accumulation of DNA damage in the aging brain.
    J Biol Chem. 285: 38534-42.
  23. Kapturczak, M.H. et al (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse.
    Am J Pathol. 165: 1045-53.
  24. Kroner, A. et al (2010) Ectopic T-cell specificity and absence of perforin and granzyme B alleviate neural damage in oligodendrocyte mutant mice.
    Am J Pathol. 176: 549-55.
  25. L'Episcopo, F. et al. (2010) Combining nitric oxide release with anti-inflammatory activity preserves nigrostriatal dopaminergic innervation and prevents motor impairment in a 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of Parkinson's disease.
    J Neuroinflammation. 7: 83.
  26. Samanta, J. et al. (2010) Noggin protects against ischemic brain injury in rodents.
    Stroke. 41: 357-62.
  27. Yang, X. et al (2010) The role of the JAK2-STAT3 pathway in pro-inflammatory responses of EMF-stimulated N9 microglial cells.
    J Neuroinflammation. 7: 54.
  28. Kondo, Y. et al. (2011) Macrophages counteract demyelination in a mouse model of globoid cell leukodystrophy.
    J Neurosci. 31: 3610-24.
  29. Macrez, R. et al. (2016) Neuroendothelial NMDA receptors as therapeutic targets in experimental autoimmune encephalomyelitis.
    Brain. Jul 19. pii: aww172. [Epub ahead of print]
  30. Amantea, D. et al. (2016) Neuroprotective Properties of a Macrolide Antibiotic in a Mouse Model of Middle Cerebral Artery Occlusion: Characterization of the Immunomodulatory Effects and Validation of the Efficacy of Intravenous Administration.
    Assay Drug Dev Technol. Jul 8. [Epub ahead of print]
  31. Werneburg, S. et al. (2016) Polysialylation and lipopolysaccharide-induced shedding of E-selectin ligand-1 and neuropilin-2 by microglia and THP-1 macrophages.
    Glia. 64 (8): 1314-30.
  32. Certo, M. et al. (2015) Activation of RXR/PPARγ underlies neuroprotection by bexarotene in ischemic stroke.
    Pharmacol Res. 102: 298-307.
  33. Chen, Z.Z. et al. (2016) Memantine mediates neuroprotection via regulating neurovascular unit in a mouse model of focal cerebral ischemia.
    Life Sci. 150: 8-14.
  34. Rich, M.C. et al. (2016) Site-targeted complement inhibition by a complement receptor 2-conjugated inhibitor (mTT30) ameliorates post-injury neuropathology in mouse brains.
    Neurosci Lett. 617: 188-94.
  35. McCarthy, R.C. et al. (2016) Characterization of a novel adult murine immortalized microglial cell line and its activation by amyloid-beta.
    J Neuroinflammation. 13: 21.
  36. Jones, R.S. et al. (2015) Inhibition of JAK2 attenuates the increase in inflammatory markers in microglia from APP/PS1 mice.
    Neurobiol Aging. 36 (10): 2716-24.
  37. Amantea, D. et al. (2016) Azithromycin protects mice against ischemic stroke injury by promoting macrophage transition towards M2 phenotype.
    Exp Neurol. 275 Pt 1: 116-25.
  38. Bains, M. & Roberts, J.L. (2016) Estrogen protects against dopamine neuron toxicity in primary mesencephalic cultures through an indirect P13K/Akt mediated astrocyte pathway.
    Neurosci Lett. 610: 79-85.
  39. Ji, J. et al. (2015) Iptakalim protects against ischemic injury by improving neurovascular unit function in the mouse brain.
    Clin Exp Pharmacol Physiol. 42 (7): 766-71.
  40. Kim, B.W. et al. (2015) α-Asarone attenuates microglia-mediated neuroinflammation by inhibiting NF kappa B activation and mitigates MPTP-induced behavioral deficits in a mouse model of Parkinson's disease.
    Neuropharmacology. 97: 46-57.
  41. Nishikawa, K. et al. (2015) Resveratrol increases CD68⁺ Kupffer cells colocalized with adipose differentiation-related protein and ameliorates high-fat-diet-induced fatty liver in mice.
    Mol Nutr Food Res. 59 (6): 1155-70.
  42. Jiang, H. et al. (2017) Dense Intra-adipose Sympathetic Arborizations Are Essential for Cold-Induced Beiging of Mouse White Adipose Tissue.
    Cell Metab. 26 (4): 686-692.e3.
  43. Zhang, J.C. et al. (2017) Prophylactic effects of sulforaphane on depression-like behavior and dendritic changes in mice after inflammation.
    J Nutr Biochem. 39: 134-44.
  44. Petković, F. et al. (2017) Reduced cuprizone-induced cerebellar demyelination in mice with astrocyte-targeted production of IL-6 is associated with chronically activated, but less responsive microglia.
    J Neuroimmunol. 310: 97-102.
  45. Olesen, M. N. et al. (2018) CD4 T cells react to local increase of α-synuclein in a pathology-associated variant-dependent manner and modify brain microglia in absence of brain pathology
    Heliyon. 4 (1): e00513.
  46. Shin, D. et al. (2018) Bee Venom Phospholipase A2 Alleviate House Dust Mite-Induced Atopic Dermatitis-Like Skin Lesions by the CD206 Mannose Receptor.
    Toxins (Basel). 10 (4)Apr 02 [Epub ahead of print].
  47. Price, B.R. et al. (2020) Therapeutic Trem2 activation ameliorates amyloid-beta deposition and improves cognition in the 5XFAD model of amyloid deposition.
    J Neuroinflammation. 17 (1): 238.
  48. Thiesler, H. et al. (2020) Polysialic acid and Siglec-E orchestrate negative feedback regulation of microglia activation.
    Cell Mol Life Sci. Jul 28 [Epub ahead of print].
  49. Lemaitre, D. et al. (2020) Collateral Sprouting of Peripheral Sensory Neurons Exhibits a Unique Transcriptomic Profile.
    Mol Neurobiol. 57 (10): 4232-49.
  50. Groh, J. et al. (2021) Immune modulation attenuates infantile neuronal ceroid lipofuscinosis in mice before and after disease onset
    Brain Communications. fcab047 [Epub ahead of print].
  51. Lim, S.L. et al. (2020) Genetic Ablation of Hematopoietic Cell Kinase Accelerates Alzheimer's Disease-Like Neuropathology in Tg2576 Mice.
    Mol Neurobiol. 57 (5): 2447-60.
  52. Dos-Santos-Pereira, M. et al. (2018) Microglial glutamate release evoked by α-synuclein aggregates is prevented by dopamine.
    Glia. 66 (11): 2353-2365.
  53. Martiskainen, H. et al. (2017) DHCR24 exerts neuroprotection upon inflammation-induced neuronal death.
    J Neuroinflammation. 14 (1): 215.
  54. Paasila, P.J. et al. (2021) Ground state depletion microscopy as a tool for studying microglia-synapse interactions.
    J Neurosci Res. 99 (6): 1515-32.
  55. Machado-Pereira, M. et al. (2022) Argonaute-2 protects the neurovascular unit from damage caused by systemic inflammation.
    J Neuroinflammation. 19 (1): 11.
  56. Karikari, A.A. et al. (2022) Neurodegeneration by α-synuclein-specific T cells in AAV-A53T-α-synuclein Parkinson's disease mice.
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    Cells. 11 (12): 1933.

Flow Cytometry

Immunofluorescence

Immunohistology - Frozen

Immunohistology - Paraffin

Synonyms
Integrin Alpha M Chain
MAC-1
RRID
AB_324898
UniProt
P05555
Entrez Gene
Itgam
GO Terms
GO:0001846 opsonin binding
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0005634 nucleus
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0030593 neutrophil chemotaxis
GO:0043395 heparan sulfate proteoglycan binding
GO:0045123 cellular extravasation
GO:0050798 activated T cell proliferation
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