CD11b antibody | M1/70.15
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IF F C P ResetRat anti Mouse CD11b:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- M1/70.15
- Isotype
- IgG2b
- Specificity
- CD11b
Rat anti Mouse CD11b antibody, clone M1/70.15 recognizes the murine CD11b cell surface antigen also known as the alpha M integrin chain or MAC-1, a differentiation antigen expressed by granulocytes, monocytes, NK cells and tissue macrophages. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to block iC3b binding to its receptor (Beller et al. 1982). Rat anti Mouse CD11b antibody, clone M1/70.15 has been reported to as being suitable for use on PLP fixed paraffin embedded tissue but has not been tested for use on formalin fixed tissue (Whiteland et al. 1995). This product is routinely tested in flow cytometry on mouse peritoneal macrophages. |
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rabbit - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide 1% Bovine Serum Albumin - Immunogen
- T cell enriched splenocytes from B10 mice.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized DA rats were fused with cells of the NS1/1.Ag4.1 mouse myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 106 cells in 100ul.
The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. Non-specific FcR binding may be reduced by using SeroBlock FcR reagent.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
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Source Reference
-
Springer, T. et al. (1978) Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.
Eur J Immunol. 8 (8): 539-51.
Antibody Characterization Reference
-
Springer, T. et al. (1979) Mac-1: a macrophage differentiation antigen identified by monoclonal antibody.
Eur J Immunol. 9 (4): 301-6.
References for CD11b antibody
-
Beller, D.I. et al. (1982) Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.
J Exp Med. 156 (4): 1000-9. -
Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
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Welt, F.G. et al. (2000) Neutrophil, not macrophage, infiltration precedes neointimal thickening in balloon-injured arteries.
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Kapturczak, M.H. et al (2004) Heme oxygenase-1 modulates early inflammatory responses: evidence from the heme oxygenase-1-deficient mouse.
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Patel, P.C. and Harrison, R.E. (2008) Membrane ruffles capture C3bi-opsonized particles in activated macrophages.
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Huang, Q.Q. et al (2008) Role of H2-calponin in regulating macrophage motility and phagocytosis.
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Brochard, V. et al (2009) Infiltration of CD4+ lymphocytes into the brain contributes to neurodegeneration in a mouse model of Parkinson disease.
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Hudcovic, T. et al (2009) Monocolonization with Bacteroides ovatus protects immunodeficient SCID mice from mortality in chronic intestinal inflammation caused by long-lasting dextran sodium sulfate treatment.
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View The Latest Product References
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- Synonyms
- Integrin Alpha M Chain
- MAC-1
- RRID
- AB_321208
- UniProt
- P05555
- Entrez Gene
- Itgam
- GO Terms
- GO:0001846 opsonin binding
- GO:0004872 receptor activity
- GO:0008305 integrin complex
- GO:0005634 nucleus
- GO:0007159 leukocyte cell-cell adhesion
- GO:0007229 integrin-mediated signaling pathway
- GO:0008201 heparin binding
- GO:0009897 external side of plasma membrane
- GO:0030593 neutrophil chemotaxis
- View More GO Terms
- GO:0043395 heparan sulfate proteoglycan binding
- GO:0045123 cellular extravasation
- GO:0050798 activated T cell proliferation
MCA74A488T
1014 154613MCA74A488
0214 148971If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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