CD11b antibody | 5C6

Product Code Applications Pack Size List Price Your Price Qty
MCA711
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SDS Safety Datasheet SDS
C F IF IP 1 mg loader
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MCA711G
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C F IF IP 0.25 mg loader
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MCA711GT
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C F IF IP 25 µg loader
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Rat anti Mouse CD11b antibody, clone 5C6 recognizes CD11b, also known as the integrin alpha M chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.

Rat anti Mouse CD11b antibody, clone 5C6 immunoprecipitates a heterodimer of ~165 and ~95 kDa. This clone also exhibits various functional properties, reportedly inhibiting adhesion in vitro and inflammatory recruitment in vivo. Rat anti Mouse CD11b antibody, clone 5C6 also inhibits delayed hypersensitivity, potentiates bacterial infections and inhibits type 1 diabetes.
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Our CD11b (5C6) Antibody has been referenced in >161 publications*


*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
MCA711, MCA711G: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
MCA711GT: Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
Thioglycollate-elicited peritoneal macrophages (TPM)
Approx. Protein Concentrations
IgG concentration 1 mg/ml
Fusion Partners
Spleen cells from AO rats were fused with cells of the Y3 rat myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/100
Immunofluorescence
Immunohistology - Frozen
Immunoprecipitation
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul
Histology Positive Control Tissue
Spleen

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Description Goat anti Rat IgG:DyLight®550 (Mouse Adsorbed)
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References for CD11b antibody

  1. Rosen, H. and Gordon, S. (1987) Monoclonal antibody to the murine type 3 complement receptor inhibits adhesion of myelomonocytic cells in vitro and inflammatory cell recruitment in vivo.
    J Exp Med. 166: 1685-701.
  2. Rosen, H. et al. (1989) Antibody to the murine type 3 complement receptor inhibits T lymphocyte-dependent recruitment of myelomonocytic cells in vivo.
    J Exp Med. 169: 535-48.
  3. Serafini, B. et al. (2000) Intracerebral recruitment and maturation of dendritic cells in the onset and progression of experimental autoimmune encephalomyelitis.
    Am J Pathol. 157: 1991-2002.
  4. Platt, N. et al. (2000) Apoptotic thymocyte clearance in scavenger receptor class A-deficient mice is apparently normal.
    J Immunol. 164: 4861-7.
  5. Carenini, S. et al. (2001) The role of macrophages in demyelinating peripheral nervous system of mice heterozygously deficient in p0.
    J Cell Biol. 152: 301-8.
  6. Engwerda, C.R. et al. (2002) Locally up-regulated lymphotoxin alpha, not systemic tumor necrosis factor alpha, is the principle mediator of murine cerebral malaria.
    J Exp Med. 195: 1371-7.
  7. Mittal, A. et al. (2003) CD11b+ cells are the major source of oxidative stress in UV radiation-irradiated skin: possible role in photoaging and photocarcinogenesis.
    Photochem Photobiol. 77 (3): 259-64.
  8. Lesnik, P. et al. (2003) Decreased atherosclerosis in CX3CR1-/- mice reveals a role for fractalkine in atherogenesis.
    J Clin Invest. 111: 333-40.
  9. View The Latest Product References
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    J Leukoc Biol. 75 (3): 453-9.
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    J Exp Med. 201: 1615-25.
  12. Mennini, T. et al. (2006) Nonhematopoietic erythropoietin derivatives prevent motoneuron degeneration in vitro and in vivo.
    Mol Med. 12: 153-60.
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  15. Halle, A. et al. (2008) The NALP3 inflammasome is involved in the innate immune response to amyloid-beta.
    Nat Immunol. 9: 857-65.
  16. Devey, L. et al. (2008) Tissue-resident Macrophages protect the Liver From Ischemia Reperfusion Injury via a Heme Oxygenase-1-Dependent mechanism.
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  22. Valerio, A. et al. (2009) Leptin is induced in the ischemic cerebral cortex and exerts neuroprotection through NF-kappaB/c-Rel-dependent transcription.
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  28. Cui, Y.F. et al. (2010) Embryonic stem cell-derived L1 overexpressing neural aggregates enhance recovery in Parkinsonian mice.
    Brain. 133: 189-204.
  29. Wu, T. et al. (2011) Expression and cellular localization of cyclooxygenases and prostaglandin E synthases in the hemorrhagic brain.
    J Neuroinflammation. 8:22.
  30. Tysseling, V.M.et al. (2011) SDF1 in the dorsal corticospinal tract promotes CXCR4+ cell migration after spinal cord injury.
    J Neuroinflammation. 8:16.
  31. McDonald, J.U. et al. (2011) In vivo functional analysis and genetic modification of in vitro-derived mouse neutrophils.
    FASEB J. 25: 1972-82.
  32. Terwel, D. et al. (2011) Critical Role of Astroglial Apolipoprotein E and Liver X Receptor-{alpha} Expression for Microglial A{beta} Phagocytosis.
    J Neurosci. 31: 7049-59.
  33. Sato, A. et al. (2012) Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury.
    J Neuroinflammation. 9: 65.
  34. Heydenreich, N. et al. (2012) C1-inhibitor protects from brain ischemia-reperfusion injury by combined antiinflammatory and antithrombotic mechanisms.
    Stroke. 43 (9): 2457-67.
  35. Yamanaka M et al. (2012) PPARγ/RXRα-induced and CD36-mediated microglial amyloid-β phagocytosis results in cognitive improvement in amyloid precursor protein/presenilin 1 mice.
    J Neurosci. 32 (48): 17321-31.
  36. Mencl, S. et al. (2014) FTY720 does not protect from traumatic brain injury in mice despite reducing posttraumatic inflammation.
    J Neuroimmunol. 274 (1-2): 125-31.
  37. Natrajan, M.S. et al. (2015) Retinoid X receptor activation reverses age-related deficiencies in myelin debris phagocytosis and remyelination.
    Brain. 138 (Pt 12): 3581-97.
  38. Kan, M.J. et al. (2015) Arginine deprivation and immune suppression in a mouse model of Alzheimer's disease.
    J Neurosci. 35 (15): 5969-82.
  39. Babcock, A.A. et al. (2015) Cytokine-producing microglia have an altered beta-amyloid load in aged APP/PS1 Tg mice.
    Brain Behav Immun. 48: 86-101.
  40. Kaindlstorfer, C. et al. (2015) Failure of Neuroprotection Despite Microglial Suppression by Delayed-Start Myeloperoxidase Inhibition in a Model of Advanced Multiple System Atrophy: Clinical Implications.
    Neurotox Res. 28 (3): 185-94.
  41. Liu, Z. et al. (2016) Transforming growth factor-β1 acts via TβR-I on microglia to protect against MPP(+)-induced dopaminergic neuronal loss.
    Brain Behav Immun. 51: 131-43.
  42. Garcia-Mesa, Y. et al. (2017) Immortalization of primary microglia: a new platform to study HIV regulation in the central nervous system.
    J Neurovirol. 23 (1): 47-66.
  43. Lu, Y. et al. (2016) Annexin A10 is involved in the development and maintenance of neuropathic pain in mice.
    Neurosci Lett. 631: 1-6.
  44. Hristova M et al. (2016) Inhibition of Signal Transducer and Activator of Transcription 3 (STAT3) reduces neonatal hypoxic-ischaemic brain damage.
    J Neurochem. 136 (5): 981-994.
  45. Ye, M. et al. (2016) Neuroprotective effects of bee venom phospholipase A2 in the 3xTg AD mouse model of Alzheimer's disease.
    J Neuroinflammation. 13 (1): 10.
  46. Nagai, J. et al. (2016) Inhibition of CRMP2 phosphorylation repairs CNS by regulating neurotrophic and inhibitory responses.
    Exp Neurol. 277: 283-95.
  47. Tachibana, M. et al. (2016) Rescuing effects of RXR agonist bexarotene on aging-related synapse loss depend on neuronal LRP1.
    Exp Neurol. 277: 1-9.
  48. Sun, H. et al. (2016) Aquaporin-4 mediates communication between astrocyte and microglia: Implications of neuroinflammation in experimental Parkinson's disease.
    Neuroscience. 317: 65-75.
  49. Shinohara, M. et al. (2016) APOE2 eases cognitive decline during Aging: Clinical and preclinical evaluations.
    Ann Neurol. 79 (5): 758-74.
  50. Kami, K. et al. (2016) Histone Acetylation in Microglia Contributes to Exercise-Induced Hypoalgesia in Neuropathic Pain Model Mice.
    J Pain. 17 (5): 588-99.
  51. Bisht, K. et al. (2016) Dark microglia: A new phenotype predominantly associated with pathological states.
    Glia. 64 (5): 826-39.
  52. Hilla, A.M. et al. (2017) Microglia Are Irrelevant for Neuronal Degeneration and Axon Regeneration after Acute Injury.
    J Neurosci. 37 (25): 6113-24.
  53. Paizs, M. et al. (2017) Axotomy Leads to Reduced Calcium Increase and Earlier Termination of CCL2 Release in Spinal Motoneurons with Upregulated Parvalbumin Followed by Decreased Neighboring Microglial Activation.
    CNS Neurol Disord Drug Targets. 16 (3): 356-67.
  54. Pulido-Salgado, M. et al. (2017) Myeloid C/EBPβ deficiency reshapes microglial gene expression and is protective in experimental autoimmune encephalomyelitis.
    J Neuroinflammation. 14 (1): 54.
  55. Thomsen, M.S. et al. (2017) Synthesis and deposition of basement membrane proteins by primary brain capillary endothelial cells in a murine model of the blood-brain barrier.
    J Neurochem. 140 (5): 741-754.
  56. Laurent, C. et al. (2017) Hippocampal T cell infiltration promotes neuroinflammation and cognitive decline in a mouse model of tauopathy.
    Brain. 140 (Pt 1): 184-200.
  57. Schuhmann, M.K. et al. (2017) Blocking of platelet glycoprotein receptor Ib reduces "thrombo-inflammation" in mice with acute ischemic stroke.
    J Neuroinflammation. 14 (1): 18.
  58. Rabl R et al. (2017) Early start of progressive motor deficits in Line 61 α-synuclein transgenic mice.
    BMC Neurosci. 18 (1): 22.
  59. Crépeaux, G. et al. (2017) Non-linear dose-response of aluminium hydroxide adjuvant particles: Selective low dose neurotoxicity.
    Toxicology. 375: 48-57.
  60. Da Ros, F. et al. (2017) Targeting Interleukin-1β Protects from Aortic Aneurysms Induced by Disrupted Transforming Growth Factor β Signaling.
    Immunity. 47 (5): 959-973.e9.
  61. Hou, L. et al. (2018) Taurine protects noradrenergic locus coeruleus neurons in a mouse Parkinson's disease model by inhibiting microglial M1 polarization.
    Amino Acids. 50 (5): 547-556.
  62. Zheng, J. et al. (2018) Evaluation of metastatic niches in distant organs after surgical removal of tumor-bearing lymph nodes.
    BMC Cancer. 18 (1): 608.
  63. Soto, M. et al. (2018) Gut microbiota modulate neurobehavior through changes in brain insulin sensitivity and metabolism.
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    Glia. 66 (8): 1736-51.
  65. Roberts, J.M. et al. (2018) Bilateral carotid artery stenosis causes unexpected early changes in brain extracellular matrix and blood-brain barrier integrity in mice.
    PLoS One. 13 (4): e0195765.
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    Brain Behav Immun. 81: 630-45.
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  75. Yang, P. et al. (2020) Suppression of cGMP-Dependent Photoreceptor Cytotoxicity With Mycophenolate Is Neuroprotective in Murine Models of Retinitis Pigmentosa.
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  79. Ellman, D.G. et al. (2020) Conditional Ablation of Myeloid TNF Improves Functional Outcome and Decreases Lesion Size after Spinal Cord Injury in Mice.
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  81. Yoshizaki, S. et al. (2021) Microglial inflammation after chronic spinal cord injury is enhanced by reactive astrocytes via the fibronectin/β1 integrin pathway.
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  82. Mañucat-Tan, N. et al. (2021) Hypochlorite-induced aggregation of fibrinogen underlies a novel antioxidant role in blood plasma.
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  83. Brunialti, E. et al. (2021) Inhibition of microglial β-glucocerebrosidase hampers the microglia-mediated antioxidant and protective response in neurons.
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  85. Ji, N. et al. (2022) VSIG4 Attenuates NLRP3 and Ameliorates Neuroinflammation via JAK2-STAT3-A20 Pathway after Intracerebral Hemorrhage in Mice.
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    Exp Neurobiol. 31 (3): 173-95.

Blocking assay

Immunofluorescence

Immunohistology - Frozen

Synonyms
Integrin Alpha M Chain
MAC-1
RRID
AB_321292
UniProt
P05555
Entrez Gene
Itgam
GO Terms
GO:0001846 opsonin binding
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0005634 nucleus
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0030593 neutrophil chemotaxis
GO:0043395 heparan sulfate proteoglycan binding
GO:0045123 cellular extravasation
GO:0050798 activated T cell proliferation

MCA711

152972

MCA711G

154201 163792 1709

MCA711GT

151040 1804

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