CD11b antibody | 5C6

Rat anti Mouse CD11b:Pacific Blue®

Product Type
Monoclonal Antibody
Clone
5C6
Isotype
IgG2b
Specificity
CD11b

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MCA711PB
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SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Rat anti Mouse CD11b antibody, clone 5C6 recognizes CD11b, also known as the integrin alpha M chain. CD11b is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles.

Rat anti Mouse CD11b antibody, clone 5C6 immunoprecipitates a heterodimer of ~165 and ~95 kDa. This clone also exhibits various functional properties, reportedly inhibiting adhesion in vitro and inflammatory recruitment in vivo. Rat anti Mouse CD11b antibody, clone 5C6 also inhibits delayed hypersensitivity, potentiates bacterial infections and inhibits type 1 diabetes.
CiteAb logo - trusted, tested, published

Our CD11b (5C6) Antibody has been referenced in >161 publications*


*Based on June 2020 data from CiteAb's antibody search engine.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG conjugated to Pacific Blue - liquid
Preparation
Purified IgG prepared by ion exchange chromatography from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
1%Bovine Serum Albumin
Immunogen
Thioglycollate-elicited peritoneal macrophages (TPM)
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from AO rats were fused with cells of the Y3 rat myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Pacific Blue® 410 455
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR ( BUF041A/B).

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Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2b Negative Control:Pacific Blue® MCA6006PB F 100 Tests/1ml loader
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Description Rat IgG2b Negative Control:Pacific Blue®

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

References for CD11b antibody

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  9. View The Latest Product References
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Blocking assay

Immunofluorescence

Immunohistology - Frozen

Synonyms
Integrin Alpha M Chain
MAC-1
UniProt
P05555
Entrez Gene
Itgam
GO Terms
GO:0001846 opsonin binding
GO:0004872 receptor activity
GO:0008305 integrin complex
GO:0005634 nucleus
GO:0007159 leukocyte cell-cell adhesion
GO:0007229 integrin-mediated signaling pathway
GO:0008201 heparin binding
GO:0009897 external side of plasma membrane
GO:0030593 neutrophil chemotaxis
GO:0043395 heparan sulfate proteoglycan binding
GO:0045123 cellular extravasation
GO:0050798 activated T cell proliferation

MCA711PB

1611

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