Catenin Alpha 1 antibody | alpha-CAT-7A4

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Mouse anti Catenin Alpha 1

Product Type
Monoclonal Antibody
Clone
alpha-CAT-7A4
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA6033 E IF IP P* WB 50 µg
Mouse anti catenin alpha 1 antibody clone alpha-CAT-7A4 recognizes catenin alpha 1 also known as alpha E-catenin, cadherin-associated protein or renal carcinoma antigen NY-REN-13.

Catenin alpha 1 is a member of the catenin protein family, which also includes the catenin beta, catenin gamma and catenin delta proteins. Catenin alpha 1 is an important actin binding protein and has also been shown to function as a tumor suppressor (Piao et al. 2014, Silvis et al. 2014).

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Human
Rat
Chicken
Xenopus laevis
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by peptide-affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide derived from the C-terminus of mouse catenin alpha 1
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
12 months from date of despatch.

More Information

UniProt
P35221 Related reagents
Entrez Gene
CTNNA1 Related reagents
GO Terms
GO:0007155 cell adhesion
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0005911 cell-cell junction
GO:0005912 adherens junction
GO:0008013 beta-catenin binding
GO:0015629 actin cytoskeleton
GO:0016342 catenin complex
GO:0017166 vinculin binding
GO:0034332 adherens junction organization
GO:0042692 muscle cell differentiation
GO:0043297 apical junction assembly
GO:0045295 gamma-catenin binding
GO:0045296 cadherin binding
GO:0051149 positive regulation of muscle cell differentiation
GO:0071681 cellular response to indole-3-methanol
Regulatory
For research purposes only

Applications of Catenin Alpha 1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.1 ug/ml 1 ug/ml
Immunofluorescence 2 ug/ml 5 ug/ml
Immunohistology - Paraffin 1 2 ug/ml 10 ug/ml
Immunoprecipitation 5 ug/ml
Western Blotting 1 ug/ml
  1. 1 Heat-induced epitope retrieval is required for IHC on formalin-fixed paraffin-embedded tissue
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Mouse anti catenin alpha 1 antibody recognizes a band of approximately 100 kDa in HeLa cell lysate

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F