Catenin Alpha 1 antibody | alpha-CAT-7A4
Mouse anti catenin alpha 1 antibody clone alpha-CAT-7A4 recognizes catenin alpha 1 also known as alpha E-catenin, cadherin-associated protein or renal carcinoma antigen NY-REN-13.
Catenin alpha 1 is a member of the catenin protein family, which also includes the catenin beta, catenin gamma and catenin delta proteins. Catenin alpha 1 is an important actin binding protein and has also been shown to function as a tumor suppressor (Piao et al. 2014, Silvis et al. 2014).
Catenin alpha 1 is a member of the catenin protein family, which also includes the catenin beta, catenin gamma and catenin delta proteins. Catenin alpha 1 is an important actin binding protein and has also been shown to function as a tumor suppressor (Piao et al. 2014, Silvis et al. 2014).
Product Details
- Target Species
- Mouse
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Rat Chicken Xenopus laevis - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by peptide-affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.1% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide derived from the C-terminus of mouse catenin alpha 1
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P35221
- Entrez Gene
- CTNNA1
- GO Terms
- GO:0007155 cell adhesion
- GO:0005829 cytosol
- GO:0005198 structural molecule activity
- GO:0005911 cell-cell junction
- GO:0005912 adherens junction
- GO:0008013 beta-catenin binding
- GO:0015629 actin cytoskeleton
- GO:0016342 catenin complex
- GO:0017166 vinculin binding
- GO:0034332 adherens junction organization
- GO:0042692 muscle cell differentiation
- GO:0043297 apical junction assembly
- GO:0045295 gamma-catenin binding
- GO:0045296 cadherin binding
- GO:0051149 positive regulation of muscle cell differentiation
- GO:0071681 cellular response to indole-3-methanol
- Regulatory
- For research purposes only
Applications of Catenin Alpha 1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | 0.1 ug/ml | 1 ug/ml | |
| Immunofluorescence | 2 ug/ml | 5 ug/ml | |
| Immunohistology - Paraffin 1 | 2 ug/ml | 10 ug/ml | |
| Immunoprecipitation | 5 ug/ml | ||
| Western Blotting | 1 ug/ml |
- 1 Heat-induced epitope retrieval is required for IHC on formalin-fixed paraffin-embedded tissue
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse anti catenin alpha 1 antibody recognizes a band of approximately 100 kDa in HeLa cell lysate
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