CaMK II Inhibitor Beta antibody | AbD06016

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Human anti CaMK II Inhibitor Beta

Product Type
Monoclonal Antibody
Clone
AbD06016
Isotype
HuCAL Fab bivalent
Product Code Applications Pack Size List Price Quantity
HCA122
Datasheet
C
MSDS
0.1 mg loader

Human anti CaMK II Inhibitor beta antibody, clone AbD06016 detects mouse calcium/calmodulin-dependent protein kinase II Inhibitor beta (CaMK2N2), also known as calcium/calmodulin-dependent protein kinase II inhibitor 2, a potent inhibitor of CaM kinase II (CAMK2). CaMK II N beta interacts with both CAMK2 alpha and beta.

The peptide sequence used to generate this antibody is identical to rat, bovine (Bos taurus) and short tailed opossum (Monodelphis sp.) CaMK II N beta.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL� phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Peptide with the sequence EDKMGRFGADPEGSDLS, corresponding to amino acids 9-25 of mouse calcium/calmodulin-dependent protein kinase II inhibitor 2 (CaMK II N beta)
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
12 months from date of reconstitution

More Information

UniProt
Q78WH7 Related reagents
A5D7T0 Related reagents
Q9Z2N6 Related reagents
Entrez Gene
CAMK2N2 Related reagents
Camk2n2 Related reagents
Camk2n2 Related reagents
GO Terms
GO:0005829 cytosol
GO:0004860 protein kinase inhibitor activity
GO:0005634 nucleus
GO:0008427 calcium-dependent protein kinase inhibitor activity
GO:0019901 protein kinase binding
GO:0048167 regulation of synaptic plasticity
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Licensed Use
For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory
For research purposes only

Applications of CaMK II Inhibitor Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Mouse brain (hippocampus). Tissue sections require incubation with antibody for 72 hours..

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Histidine Tag:Alk.Phos. MCA1396A E WB 0.1 mg
Mouse anti Histidine Tag:Biotin MCA1396B E WB 0.1 mg loader
Mouse anti c-Myc:Alk.Phos. MCA2200A C E P WB* 0.1 mg
Mouse anti c-Myc:Biotin MCA2200B C E P WB* 0.1 mg loader
Mouse anti c-Myc:HRP MCA2200P C E P WB* 0.1 mg loader
Mouse anti Penta Histidine Tag:HRP MCA5995P E WB 125 µl loader

Product Specific References

References for CaMK II Inhibitor Beta antibody

  1. Radwanska. K. et al. (2010) Differential regulation of CaMKII inhibitor beta protein expression after exposure to a novel context and during contextual fear memory formation.
    Genes Brain Behav. 9: 648-57.
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