CaMK II Inhibitor Beta antibody | AbD06016

Human anti CaMK II Inhibitor Beta

Product Type
Monoclonal Antibody
Clone
AbD06016
Isotype
HuCAL Fab bivalent
Specificity
CaMK II Inhibitor Beta

Product Code Applications Pack Size List Price Your Price Qty
HCA122
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SDS Safety Datasheet SDS
C 0.1 mg loader
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Human anti CaMK II Inhibitor beta antibody, clone AbD06016 detects mouse calcium/calmodulin-dependent protein kinase II Inhibitor beta (CaMK2N2), also known as calcium/calmodulin-dependent protein kinase II inhibitor 2, a potent inhibitor of CaM kinase II (CAMK2). CaMK II N beta interacts with both CAMK2 alpha and beta.

The peptide sequence used to generate this antibody is identical to rat, bovine (Bos taurus) and short tailed opossum (Monodelphis sp.) CaMK II N beta.

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine Expected from Sequence
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water.
Preparation
Metal chelate affinity chromatography.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Peptide with the sequence EDKMGRFGADPEGSDLS, corresponding to amino acids 9-25 of mouse calcium/calmodulin-dependent protein kinase II inhibitor 2 (CaMK II N beta)
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
His-tag is a trademark of EMD Biosciences.

Prior to reconstitution store at +4°C.
After reconstitution store at -20°C.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual.
Histology Positive Control Tissue
Mouse brain (hippocampus).

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References for CaMK II Inhibitor Beta antibody

  1. Radwanska. K. et al. (2010) Differential regulation of CaMKII inhibitor beta protein expression after exposure to a novel context and during contextual fear memory formation.
    Genes Brain Behav. 9: 648-57.

RRID
AB_10672763
UniProt
Q78WH7
A5D7T0
Q9Z2N6
Entrez Gene
CAMK2N2
Camk2n2
Camk2n2
GO Terms
GO:0005829 cytosol
GO:0004860 protein kinase inhibitor activity
GO:0005634 nucleus
GO:0008427 calcium-dependent protein kinase inhibitor activity
GO:0019901 protein kinase binding
GO:0048167 regulation of synaptic plasticity
Licensed Use
For in vitro. research purposes only, unless otherwise specified in writing by Bio-Rad.