CaMK II Inhibitor Alpha antibody | AbD06603

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Human anti CaMK II Inhibitor Alpha

Product Type
Monoclonal Antibody
Clone
AbD06603
Isotype
HuCAL Fab bivalent
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
HCA121 C 0.1 mg
CaMKII comprises of multiple isoforms that are derived from four closely related gene products; alpha, beta, gamma and delta, that have distinct but overlapping expression patterns.

Human anti CaMK II Inhibitor alpha antibody, clone AbD06603 detects mouse calcium/calmodulin-dependent protein kinase II alpha (CaMK II alpha), a serine/threonine kinase that is activated by calcium ions (Ca2+) and the Ca2+/calmodulin complex (CaM). CaMK II is expressed in glutamatergic excitatory neurones and constitutes the major postsynaptic density protein. It is important in the regulation of synaptic transmission, with possible roles in learning and memory.

The peptide sequence used to generate this antibody is identical to rat CAMK II alpha.

Product Details

Target Species
Mouse
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat Expected from Sequence
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
A bivalent human recombinant Fab selected from the HuCAL� phage display library, expressed in E. coli. This Fab fragment is dimerized via a helix-turn-helix motif. The antibody is tagged with a myc-tag (EQKLISEEDL) and a his-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied lyophilized.
Reconstitution
Reconstitute with 1.0 ml distilled water
Preparation
Metal chelate affinity chromatography
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Peptide sequence C-DEKLSPYGDGGDVGQI that corresponds to amino acids 10-25 of mouse calcium/calmodulin-dependent protein kinase II inhibitor 1 (CaMK II N alpha)
Approx. Protein Concentrations
Ig concentration 0.1 mg/ml after reconstitution

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Guarantee
12 months from date of reconstitution

More Information

UniProt
Q9JI15 Related reagents
Q6QWF9 Related reagents
Entrez Gene
Camk2n1 Related reagents
Camk2n1 Related reagents
GO Terms
GO:0014069 postsynaptic density
GO:0019717 synaptosome
GO:0030054 cell junction
GO:0045211 postsynaptic membrane
GO:0007268 synaptic transmission
GO:0008427 calcium-dependent protein kinase inhibitor activity
GO:0019901 protein kinase binding
GO:0030425 dendrite
GO:0043025 neuronal cell body
Acknowledgements
Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of CaMK II Inhibitor Alpha antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Technical Advice
Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Immunohistology
This product does not require protein digestion pre-treatment of paraffin sections. This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Histology Positive Control Tissue
Mouse brain (hippocampus)

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Mouse anti Histidine Tag:Biotin MCA1396B 0.1 mg E WB
Mouse anti c-Myc:Alk.Phos. MCA2200A 0.1 mg C E P WB*
Mouse anti c-Myc:Biotin MCA2200B 0.1 mg C E P WB*
Mouse anti c-Myc:HRP MCA2200P 0.1 mg C E P WB*
Mouse anti Penta Histidine Tag:HRP MCA5995P 125 µl E WB

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Histar Detection System STAR3000B 150 Tests C P

Product Specific References

References for CaMK II Inhibitor Alpha antibody

  1. Radwańska, K. et al (2010) Differential regulation of CaMKII inhibitor beta protein expression after exposure to a novel context and during contextual fear memory formation.
    Genes Brain Behav. 9: 648-657.
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