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ABCA1 antibody | 5A1-1422

Rat anti Mouse ABCA1:RPE

Product Type
Monoclonal Antibody
Clone
5A1-1422
Isotype
IgG2a
Specificity
ABCA1

Product Code Applications Pack Size List Price Your Price Qty
MCA2681PE
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests loader
List Price Your Price
loader

Rat anti Mouse ABCA1 antibody, clone 5A1.1422 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease.

Mutations in ABCA1 gene have been associated with Tangier disease, a genetic disorder of lipid metabolism, and the more common Familial HDL deficiency, peresenting with many of the same symproms.

Target Species
Mouse
Product Form
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilized
Reconstitution
Reconstitute with 1ml distilled water
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
5% Sucrose
Immunogen
ABCA1 transfected HeLa cells.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
RPE 488nm laser 496 578
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at +4oC. DO NOT FREEZE.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

How to Use the Spectraviewer

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  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:RPE MCA1212PE F 100 Tests
>
List Price Your Price
Description Rat IgG2a Negative Control:RPE

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
List Price Your Price
loader
Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
List Price Your Price
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Description Mouse Seroblock FcR

References for ABCA1 antibody

  1. Pradel, L.C. et al. (2009) ATP-binding cassette transporter hallmarks tissue macrophages and modulates cytokine-triggered polarization programs.
    Eur J Immunol. 39 (8): 2270-80.
  2. Wang, Y. et al. (2019) Smooth Muscle Cells Contribute the Majority of Foam Cells in ApoE (Apolipoprotein E)-Deficient Mouse Atherosclerosis.
    Arterioscler Thromb Vasc Biol. 39 (5): 876-87.

Further Reading

  1. Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function.
    Pflugers Arch. 453 (5): 569-79.

RRID
AB_2220006
UniProt
P41233
Entrez Gene
Abca1
GO Terms
GO:0002790 peptide secretion
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005548 phospholipid transporter activity
GO:0005794 Golgi apparatus
GO:0006497 protein lipidation
GO:0006911 phagocytosis, engulfment
GO:0008203 cholesterol metabolic process
GO:0008509 anion transmembrane transporter activity
GO:0016887 ATPase activity
GO:0010875 positive regulation of cholesterol efflux
GO:0017127 cholesterol transporter activity
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0043691 reverse cholesterol transport
GO:0045332 phospholipid translocation
GO:0071222 cellular response to lipopolysaccharide
GO:0071300 cellular response to retinoic acid

MCA2681PE

151224

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