ABCA1 antibody | 5A1-1422

Rat anti Mouse ABCA1:FITC

Product Type
Monoclonal Antibody
Clone
5A1-1422
Isotype
IgG2a
Specificity
ABCA1

Product Code Applications Pack Size List Price Your Price Qty
MCA2681F
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F 0.1 mg loader
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Rat anti Mouse ABCA1 antibody, clone 5A1.1422 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease.

Mutations in ABCA1 gene have been associated with Tangier disease, a genetic disorder of lipid metabolism, and the more common Familial HDL deficiency, peresenting with many of the same symproms.

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
ABCA1 transfected HeLa cells.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rat IgG2a Negative Control:FITC MCA1212F F 100 Tests loader
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Description Rat IgG2a Negative Control:FITC

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse Seroblock FcR BUF041A F 0.1 mg loader
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Description Mouse Seroblock FcR
Mouse Seroblock FcR BUF041B F 0.5 mg loader
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Description Mouse Seroblock FcR

References for ABCA1 antibody

  1. Pradel, L.C. et al. (2009) ATP-binding cassette transporter hallmarks tissue macrophages and modulates cytokine-triggered polarization programs.
    Eur J Immunol. 39 (8): 2270-80.
  2. Wang, Y. et al. (2019) Smooth Muscle Cells Contribute the Majority of Foam Cells in ApoE (Apolipoprotein E)-Deficient Mouse Atherosclerosis.
    Arterioscler Thromb Vasc Biol. : ATVBAHA119312434. [Epub ahead of print]

Further Reading

  1. Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function.
    Pflugers Arch. 453 (5): 569-79.

RRID
AB_1604581
UniProt
P41233
Entrez Gene
Abca1
GO Terms
GO:0002790 peptide secretion
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005548 phospholipid transporter activity
GO:0005794 Golgi apparatus
GO:0006497 protein lipidation
GO:0006911 phagocytosis, engulfment
GO:0008203 cholesterol metabolic process
GO:0008509 anion transmembrane transporter activity
GO:0016887 ATPase activity
GO:0010875 positive regulation of cholesterol efflux
GO:0017127 cholesterol transporter activity
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0043691 reverse cholesterol transport
GO:0045332 phospholipid translocation
GO:0071222 cellular response to lipopolysaccharide
GO:0071300 cellular response to retinoic acid
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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