ABCA1 antibody | 3A1-891.3

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Rat anti Mouse ABCA1:FITC

Product Type
Monoclonal Antibody
Clone
3A1-891.3
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA2878F F 0.1 mg
Rat anti Mouse ABCA1 antibody, clone 3A1-891.3 recognizes murine adenosine triphosphate (ATP) Binding cassette transporter 1 (ABCA1). The ABC transporters are a large family of conserved proteins that transport a wide variety of molecules across cellular membranes. ABCA1 is a member of the ABC-A sub-family, which acts as a lipid translocator. The molecule was originally identified as a scavenger receptor on macrophages and research shows that ABCA1 also plays a major role in cholesterol metabolism. ABCA1 may play an important role in protecting against cardiovascular disease.

Mutations in ABCA1 gene have been associated with Tangiers disease, a genetic disorder of lipid metabolism, and familial high density lipoprotein (HDL) deficiency.

Product Details

Target Species
Mouse
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Synthetic peptide corresponding to aa 215-233
Approx. Protein Concentrations
IgG concentration 0.1mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
18 months from date of despatch.

More Information

UniProt
P41233 Related reagents
Entrez Gene
Abca1 Related reagents
GO Terms
GO:0002790 peptide secretion
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0005548 phospholipid transporter activity
GO:0005794 Golgi apparatus
GO:0006497 protein lipidation
GO:0006911 phagocytosis, engulfment
GO:0008203 cholesterol metabolic process
GO:0008509 anion transmembrane transporter activity
GO:0016887 ATPase activity
GO:0010875 positive regulation of cholesterol efflux
GO:0017127 cholesterol transporter activity
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0043691 reverse cholesterol transport
GO:0045332 phospholipid translocation
GO:0071222 cellular response to lipopolysaccharide
GO:0071300 cellular response to retinoic acid
Regulatory
For research purposes only

Applications of ABCA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Mouse Seroblock FcR BUF041A 0.1 mg F
Mouse Seroblock FcR BUF041B 0.5 mg F

Product Specific References

References for ABCA1 antibody

  1. Zarubica, A. et al. (2007) ABCA1, from pathology to membrane function.
    Pflugers Arch. 453 (5): 569-79.
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