Leu-Enkephalin antibody | NOC.1

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Mouse anti Leu-Enkephalin

Product Type
Monoclonal Antibody
Clone
NOC.1
Isotype
IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
4140-0355 C R 0.1 ml
Mouse anti Human Leu5-enkephalin antibody, clone NOC.1 recognizes both Met5- and Leu5-enkephalin, secreted peptides which mimic the effect of opiate drugs. Mouse anti Human Leu5-enkephalin antibody, clone NOC.1 recognizes well established enkephalin immunoreactive sites, but does not bind to areas known to contain beta-endorphin or dynorphin in IHC.

Cross reactivity with Met5-enkephalin and lack of reactivity with the peptide Gly-Gly-Phe-Leu and other related peptides by inhibition of radiolabelled Leu5-enkephalin binding is noted (Andersson et al. 1995).

Product Details

Species Cross-Reactivity
Target SpeciesCross Reactivity
Pig
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Tissue Culture Supernatant - liquid
Preservative Stabilisers
0.05% Thiomersal
Immunogen
Leu5-enkaphalin cinjugated to bovine serum albumin..

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted.
Avoid repeated freezing and thawing as this may denature the antibody.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01210 Related reagents
Entrez Gene
PENK Related reagents
GO Terms
GO:0001515 opioid peptide activity
GO:0005576 extracellular region
GO:0005184 neuropeptide hormone activity
GO:0007218 neuropeptide signaling pathway
Regulatory
For research purposes only

Applications of Leu-Enkephalin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/100 1/400
Radioimmunoassays
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG1:HRP HCA036P 0.1 mg E
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Human anti Mouse IgG2b:FITC HCA038F 0.1 mg F
Human anti Mouse IgG2b:HRP HCA038P 0.1 mg E
Human anti Mouse IgG3:FITC HCA039F 0.1 mg F
Human anti Mouse IgG3:HRP HCA039P 0.1 mg E
Human anti Mouse IgG3:RPE HCA039PE 100 Tests F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Leu-Enkephalin antibody

  1. Cuello, A. C. et al. (1984) Characterization and immunocytochemical application of monoclonal antibodies against enkephalins
    J Histochem Cytochem 32: 947 -957
  2. Kaleczyc, J. et al. (2002) Distribution, immunohistochemical characteristics and nerve pathways of primary sensory neurons supplying the porcine vas deferens.
    Cell Tissue Res. 310: 9-17.
  3. Bulc, M. et al. (2014) Immunohistochemical distribution of cocaine and amphetamine regulatory peptide-like immunoreactive (CART-LI) nerve fibers in the circular muscle layer and their relationship to other peptides in the human caecum.
    Acta Histochem. 116: 1029-36.
  4. Gańko, M. & Całka, J. (2014) Localization and chemical coding of the dorsal motor vagal nucleus (DMX) neurons projecting to the porcine stomach prepyloric area in the physiological state and after stomach partial resection.
    J Mol Neurosci. 52 (1): 90-100.
  5. Sienkiewicz, W. et al. (2010) Immunohistochemical characterization of neurones in the hypoglossal nucleus of the pig.
    Anat Histol Embryol. 39 (2): 152-9.
  6. Palus, K. & Całka, J. (2015) Alterations of neurochemical expression of the coeliac-superior mesenteric ganglion complex (CSMG) neurons supplying the prepyloric region of the porcine stomach following partial stomach resection.
    J Chem Neuroanat. 72: 25-33.
  7. Gańko, M. & Całka, J. (2014) Prolonged acetylsalicylic-acid-supplementation-induced gastritis affects the chemical coding of the stomach innervating vagal efferent neurons in the porcine dorsal motor vagal nucleus (DMX).
    J Mol Neurosci. 54 (2): 188-98.