ZAP 70 antibody

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Anti ZAP 70 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with ZAP 70 antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 7 second exposure. Arrow points to ZAP 70 (molecular weight 70 kDa)

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Mouse anti ZAP 70

Product Type: PrecisionAb Monoclonal
Isotype: IgG1
Format: Purified
Specificity: ZAP 70

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Mouse anti Human ZAP 70 antibody recognizes human ZAP-70, a 70 kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation.

ZAP-70 is primarily expressed in T-cells and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia.

Mouse anti Human ZAP 70 antibody recognizes ZAP 70 as a 70 kDa band in western blotting on Jurkat and MOLT-4 cell lysates.

Target Species: Human
Western Blotting: Anti ZAP 70 antibody detects a band of approximately 70 kDa in Jurkat cell lysates.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant.
Buffer Solution: Borate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Human ZAP 70 amino acids residues 280-309
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
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Description	Goat anti Mouse IgG (H/L):HRP

UniProt: P43403
Entrez Gene: ZAP70
GO Terms: GO:0005829 cytosol; GO:0004715 non-membrane spanning protein tyrosine kinase activity; GO:0005524 ATP binding; GO:0006955 immune response; GO:0007243 intracellular protein kinase cascade; GO:0042101 T cell receptor complex; GO:0045059 positive thymic T cell selection; GO:0050852 T cell receptor signaling pathway