ZAP 70 antibody
Mouse anti ZAP 70
ZAP 70 antibody recognizes tyrosine-protein kinase ZAP-70, also known as 70 kDa zeta-chain associated protein or Syk-related tyrosine kinase. The antibody has been extensively validated for western blotting using whole cell lysates.
ZAP 70 Antibody with Control Lysate
ZAP 70 antibody recognizes tyrosine-protein kinase ZAP-70, also known as 70 kDa zeta-chain associated protein or Syk-related tyrosine kinase. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00059 | WB | 100 µl | ||||
| VMA00059KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human ZAP 70 antibody recognizes human ZAP-70, a 70 kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation.
ZAP-70 is primarily expressed in T-cells and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia.
Mouse anti Human ZAP 70 antibody recognizes ZAP 70 as a 70 kDa band in western blotting on Jurkat and MOLT-4 cell lysates.
ZAP-70 is primarily expressed in T-cells and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia.
Mouse anti Human ZAP 70 antibody recognizes ZAP 70 as a 70 kDa band in western blotting on Jurkat and MOLT-4 cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti ZAP 70 antibody detects a band of approximately 70 kDa in Jurkat cell lysates.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Borate buffered saline
- Buffer Solution
- Borate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Human ZAP 70 amino acids residues 280-309
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P43403 Related reagents
- Entrez Gene
- ZAP70 Related reagents
- GO Terms
- GO:0005829 cytosol
- GO:0004715 non-membrane spanning protein tyrosine kinase activity
- GO:0005524 ATP binding
- GO:0006955 immune response
- GO:0007243 intracellular protein kinase cascade
- GO:0042101 T cell receptor complex
- GO:0045059 positive thymic T cell selection
- GO:0050852 T cell receptor signaling pathway
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of ZAP 70 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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