ZAP 70 antibody | SBZAP

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Mouse anti Human ZAP 70:FITC

Product Type
Monoclonal Antibody
Clone
SBZAP
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
1065002S F* 25 µg
Mouse anti Human ZAP 70 antibody, clone SBZAP recognizes human ZAP-70, a 70kDa tyrosine protein kinase which associates with the T-cell receptor (TCR) zeta chain and undergoes phosphorylation following TCR stimulation. ZAP-70 is primarily expressed in T lymphocytes and natural killer cells, where it plays a key role in T-cell receptor (TCR) signalling. Recent studies suggest that ZAP-70 is also expressed in a population of normal immature B-cells and in B-cells from a sub-set of patients with chronic lymphocytic leukaemia.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to amino acids 280-309 of human ZAP70 conjugated to keyhole limpet hemocyanin (KLH).
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. This product should be stored undiluted.
This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P43403 Related reagents
Entrez Gene
ZAP70 Related reagents
GO Terms
GO:0005829 cytosol
GO:0004715 non-membrane spanning protein tyrosine kinase activity
GO:0005524 ATP binding
GO:0006955 immune response
GO:0007243 intracellular protein kinase cascade
GO:0042101 T cell receptor complex
GO:0045059 positive thymic T cell selection
GO:0050852 T cell receptor signaling pathway
Regulatory
For research purposes only

Applications of ZAP 70 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1
  1. 1Membrane permeabilisation is required for this application. Bio-Rad recommends the use of Leucoperm (Product Code BUF09) for this purpose.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Negative Isotype Controls Available

Description Product Code Pack Size Applications List Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F 100 Tests F

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Human Seroblock BUF070A 50 Test F
Human Seroblock BUF070B 200 Test F
Leucoperm™ BUF09 50 Tests F
Leucoperm™ BUF09B 250 Tests F
Leucoperm™ BUF09C 1000 Tests F

Product Specific References

References for ZAP 70 antibody

  1. Roifman, C.M. et al. (1989) Depletion of CD8+ cells in human thymic medulla results in selective immune deficiency.
    J Exp Med. 170 (6): 2177-82.
  2. Arpaia, E. et al. (1994) Defective T cell receptor signaling and CD8+ thymic selection in humans lacking zap-70 kinase.
    Cell. 76 (5): 947-58.