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YY1 antibody | 39A10-G2

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Anti YY1 Antibody, clone 39A10-G2 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with YY1 antibody (1/1,000, VMA00868) followed by detection with HRP conjugated Goat anti Mouse IgG (H/L) (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to YY1 (molecular weight 65 kDa).

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Mouse anti YY1

Product Type
PrecisionAb Monoclonal
Clone
39A10-G2
Isotype
IgG2a
Format
Purified
Specificity
YY1

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VMA00868
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Mouse anti Human YY1 antibody recognizes the transcriptional repressor protein YY1, also known as INO80 complex subunit S or Yin and Yang 1 protein.

YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1 (provided by RefSeq, Jul 2008).

Mouse anti Human YY1 antibody detects a band of 65 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti YY1 detects a band of approximately 65 kDa
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant human YY1 (amino acids 1 - 414)
Approx. Protein Concentrations
IgG concentration 0.5 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
P25490
Entrez Gene
YY1
GO Terms
GO:0005886 plasma membrane
GO:0005515 protein binding
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003713 transcription coactivator activity
GO:0003714 transcription corepressor activity
GO:0008270 zinc ion binding
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010843 promoter binding
GO:0016363 nuclear matrix
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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