Xpf antibody | UMAB20
Mouse anti Human XPF antibody recognizes DNA repair endonuclease XPF, also known as DNA excision repair protein ERCC4, RAD1, FANCQ, DNA repair protein complementing XP-F cells , XFEPS and xeroderma pigmentosum, complementation group F.
Encoded by the ERCC4 gene, DNA repair endonuclease XPF forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in ERCC4 are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6) (provided by RefSeq, Mar 2009).
Mouse anti Human XPF antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Encoded by the ERCC4 gene, DNA repair endonuclease XPF forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in ERCC4 are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6) (provided by RefSeq, Mar 2009).
Mouse anti Human XPF antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti XPF detects a band of approximately 110 kDa in HEK293 cell lysates
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human XPF (NP_005227) produced in HEK293 cells
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q92889 Related reagents
- Entrez Gene
- ERCC4 Related reagents
- GO Terms
- GO:0000014 single-stranded DNA specific endodeoxyribonuclease activity
- GO:0000110 nucleotide-excision repair factor 1 complex
- GO:0000710 meiotic mismatch repair
- GO:0000712 resolution of meiotic recombination intermediates
- GO:0000718 nucleotide-excision repair, DNA damage removal
- GO:0000724 double-strand break repair via homologous recombination
- GO:0000784 nuclear chromosome, telomeric region
- GO:0003684 damaged DNA binding
- GO:0003697 single-stranded DNA binding
- GO:0005654 nucleoplasm
- GO:0006283 transcription-coupled nucleotide-excision repair
- GO:0006295 nucleotide-excision repair, DNA incision, 3'-to lesion
- GO:0006296 nucleotide-excision repair, DNA incision, 5'-to lesion
- GO:0008022 protein C-terminus binding
- GO:0010834 telomere maintenance via telomere shortening
- GO:0032205 negative regulation of telomere maintenance
- GO:0047485 protein N-terminus binding
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Xpf antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
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