Xpf antibody | UMAB20

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Mouse anti Xpf

Mouse anti human XPF antibody recognizes the DNA repair endonuclease XPF, also known as DNA excision repair protein ERCC4, RAD1, FANCQ or DNA repair protein complementing XP-F cells. This antibody has been extensively validated for western blotting using whole cell lysates.

Xpf Antibody with Control Lysate

Mouse anti human XPF antibody recognizes the DNA repair endonuclease XPF, also known as DNA excision repair protein ERCC4, RAD1, FANCQ or DNA repair protein complementing XP-F cells. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
UMAB20
Isotype
IgG2a
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00363 WB 100 µl
VMA00363KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human XPF antibody recognizes DNA repair endonuclease XPF, also known as DNA excision repair protein ERCC4, RAD1, FANCQ, DNA repair protein complementing XP-F cells , XFEPS and xeroderma pigmentosum, complementation group F.

Encoded by the ERCC4 gene, DNA repair endonuclease XPF forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in ERCC4 are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6) (provided by RefSeq, Mar 2009).

Mouse anti Human XPF antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti XPF detects a band of approximately 110 kDa in HEK293 cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human XPF (NP_005227) produced in HEK293 cells
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch

More Information

UniProt
Q92889 Related reagents
Entrez Gene
ERCC4 Related reagents
GO Terms
GO:0000014 single-stranded DNA specific endodeoxyribonuclease activity
GO:0000110 nucleotide-excision repair factor 1 complex
GO:0000710 meiotic mismatch repair
GO:0000712 resolution of meiotic recombination intermediates
GO:0000718 nucleotide-excision repair, DNA damage removal
GO:0000724 double-strand break repair via homologous recombination
GO:0000784 nuclear chromosome, telomeric region
GO:0003684 damaged DNA binding
GO:0003697 single-stranded DNA binding
GO:0005654 nucleoplasm
GO:0006283 transcription-coupled nucleotide-excision repair
GO:0006295 nucleotide-excision repair, DNA incision, 3'-to lesion
GO:0006296 nucleotide-excision repair, DNA incision, 5'-to lesion
GO:0008022 protein C-terminus binding
GO:0010834 telomere maintenance via telomere shortening
GO:0032205 negative regulation of telomere maintenance
GO:0047485 protein N-terminus binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory
For research purposes only

Applications of Xpf antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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