Xpf antibody | UMAB20

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Anti Xpf Antibody, clone UMAB20 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with XPF antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 9 second exposure. Arrow points to XPF (molecular weight 110 kDa)

Mouse anti Xpf

Mouse anti human XPF antibody recognizes the DNA repair endonuclease XPF, also known as DNA excision repair protein ERCC4, RAD1, FANCQ or DNA repair protein complementing XP-F cells. This antibody has been extensively validated for western blotting using whole cell lysates.

Xpf Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: UMAB20
Isotype: IgG2a

Product Code	Applications	Pack Size
VMA00363	WB	100 µl
VMA00363KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human XPF antibody recognizes DNA repair endonuclease XPF, also known as DNA excision repair protein ERCC4, RAD1, FANCQ, DNA repair protein complementing XP-F cells , XFEPS and xeroderma pigmentosum, complementation group F.

Encoded by the ERCC4 gene, DNA repair endonuclease XPF forms a complex with ERCC1 and is involved in the 5' incision made during nucleotide excision repair. This complex is a structure specific DNA repair endonuclease that interacts with EME1. Defects in ERCC4 are a cause of xeroderma pigmentosum complementation group F (XP-F), or xeroderma pigmentosum VI (XP6) (provided by RefSeq, Mar 2009).

Mouse anti Human XPF antibody detects a band of 110 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human XPF (NP_005227) produced in HEK293 cells

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch

More Information

UniProt: Q92889 Related reagents
Entrez Gene: ERCC4 Related reagents
GO Terms: GO:0000014 single-stranded DNA specific endodeoxyribonuclease activity; GO:0000110 nucleotide-excision repair factor 1 complex; GO:0000710 meiotic mismatch repair; GO:0000712 resolution of meiotic recombination intermediates; GO:0000718 nucleotide-excision repair, DNA damage removal; GO:0000724 double-strand break repair via homologous recombination; GO:0000784 nuclear chromosome, telomeric region; GO:0003684 damaged DNA binding; GO:0003697 single-stranded DNA binding; GO:0005654 nucleoplasm; GO:0006283 transcription-coupled nucleotide-excision repair; GO:0006295 nucleotide-excision repair, DNA incision, 3'-to lesion; GO:0006296 nucleotide-excision repair, DNA incision, 5'-to lesion; GO:0008022 protein C-terminus binding; GO:0010834 telomere maintenance via telomere shortening; GO:0032205 negative regulation of telomere maintenance; GO:0047485 protein N-terminus binding
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories
Regulatory: For research purposes only

Applications of Xpf antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Western Blotting: Anti XPF detects a band of approximately 110 kDa in HEK293 cell lysates
Western Blotting: Anti XPF detects a band of approximately 110 kDa in HEK293 cell lysates
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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