WASL antibody | E02/4E10
Mouse anti WASL antibody recognizes WAS/WASL-interacting protein family member 1, also known as WIPF1, PRPL-2, WIP. WASL is an important regulator of actin cytoskeleton assembly and remodelling, binding to and stabilizing the actin regulator Wiskott-Aldrich syndrome protein (WASp) (Ramesh et al. 1997). WASL also interacts with actin directly, an interaction found to be critical for plasticity of the actin cytoskeleton (Keppler et al. 2018). Through its role in actin regulation, it is involved with the activation and proliferation of cells. In its wider role, WASL is a multi-tasking protein within a network of protein-protein interactions (Sokolik et al. 2020). WASL is important for immune responses which depend on cellular functions requiring actin polymerization. Different roles for WASL have been identified in B cells, T cells, and natural killer cells. In these cell types, WASL deficiency has different consequences for cell proliferation, activation and function. Mutations in the WASL binding site cause Wiskott-Aldrich syndrome (WAS) and X-linked thrombocytopenia (XLT) (Noy et al. 2012).
Product Details
- Target Species
- Human
- Western Blotting
- Mouse anti WASL detects a band of approximately 67 kDa in MCF-7 cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - Liquid
- Preparation
- Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide
- Immunogen
- E. coli-derived recombinant protein of amino acids 1-505 of human WASL
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Storage Information
- Storage
- Store undiluted at -20°C, avoiding repeated freeze thaw cycles
- Guarantee
- 12 months from date of despatch
More Information
- UniProt
- O43516
- Entrez Gene
- WIPF1
- GO Terms
- GO:0003779 actin binding
- GO:0005522 profilin binding
- GO:0006461 protein complex assembly
- GO:0008154 actin polymerization or depolymerization
- GO:0016023 cytoplasmic membrane-bounded vesicle
- Acknowledgements
- PrecisionAb is a trademark of Bio-Rad Laboratories
- Regulatory
- For research purposes only
Applications of WASL antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/2000 |
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | WB | 2 ml |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
