Vitronectin antibody | BV2

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Mouse anti Human Vitronectin

Product Type: Monoclonal Antibody
Clone: BV2
Isotype: IgG1

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
OBT1722	C E F IP WB			0.1 mg

Mouse anti Human vitronectin antibody, clone BV2 recognizes human vitronectin, also known as S-protein, serum spreading factor or V75. Vitronectin is a 459 amino acid ~55 kDa cell adhesion and spreading factor found in serum and tissue.

Product Details

Target Species: Human
Product Form: Purified IgG - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.05% Sodium Azide (NaN₃)
Immunogen: Purified human plasma vitronectin.
Approx. Protein Concentrations: IgG concentration 0.1 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 18 months from date of despatch.

More Information

UniProt: P04004 Related reagents
Entrez Gene: VTN Related reagents
GO Terms: GO:0005044 scavenger receptor activity; GO:0005178 integrin binding; GO:0005615 extracellular space; GO:0006955 immune response; GO:0008201 heparin binding; GO:0010951 negative regulation of endopeptidase activity; GO:0014911 positive regulation of smooth muscle cell migration; GO:0030195 negative regulation of blood coagulation; GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway; GO:0032092 positive regulation of protein binding; GO:0033627 cell adhesion mediated by integrin; GO:0048260 positive regulation of receptor-mediated endocytosis; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0061302 smooth muscle cell-matrix adhesion; GO:0071062 alphav-beta3 integrin-vitronectin complex; GO:0090303 positive regulation of wound healing
Regulatory: For research purposes only

Applications of Vitronectin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Flow Cytometry
Immunohistology - Frozen
Immunoprecipitation
Western Blotting

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG1:HRP	HCA036P	0.1 mg	E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Negative Isotype Controls Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Mouse IgG1 Negative Control	MCA928	100 Tests	F

Product Specific References

Source Reference

Zanetti, A. et al. (1994) Clustering of vitronectin and RGD peptides on microspheres leads to engagement of integrins on the luminal aspect of endothelial cell membrane.
Blood. 84 (4): 1116-23.

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