Vitamin D Receptor antibody | 2F4
Mouse anti Human vitamin D receptor antibody, clone 2F4 (MCA3543Z) used to evaluate VDR expression in breast cancer tissue by immunohistochemistry.
Image caption:
VDR, RXR and PPARγ immunostaining in BRCA1 mutated breast cancer. VDR, RXR and PPARγ were detected by immunohistochemistry in BRCA1 mutated breast cancer. Representative images are shown; magnification: a-c: 10 × lens, d-f: 25 × lens
From:Heublein S, Mayr D, Meindl A, Kircher A, Jeschke U, Ditsch N.
Vitamin D receptor, Retinoid X receptor and peroxisome proliferator-activated receptor γ are overexpressed in BRCA1 mutated breast cancer and predict prognosis.
J Exp Clin Cancer Res. 2017 Apr 20;36(1):57.
Mouse anti Human vitamin D receptor antibody, clone 2F4 used for the detection of vitamin D receptor expressing cells in breast cancer tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Representative immunohistochemical figures of ductal infiltrating breast cancer with low VDR expression (score 3) (a) and with high VDR expression (score 8) (b). Scale bar 30 μ. (c) The MCA graph demonstrated that phenotype AA is located in the quadrant containing the most aggressive parameters (T3 tumours, HER2 positive, ER negative and G3 in contrast to phenotype GG which is associated to more favorable bio-pathological parameters [T1 tumours, N0, G1, ki67 (-)].
From: Pulito C, Terrenato I, Di Benedetto A, Korita E, Goeman F, et al. (2015)
Cdx2 Polymorphism Affects the Activities of Vitamin D Receptor in Human Breast Cancer Cell Lines and Human Breast Carcinomas.
PLoS ONE 10(4): e0124894.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Human Vitamin D receptor antibody, clone 2F4 (MCA3543Z) used to detect vitamine D receptor expression in cancerous tissues by immunohistochemistry on formalin fixed, paraffin embedded tissue sections.
Image caption:
Detection of VDR with immunohistochemistry: a high VDR cytoplasm staining (IRS > 2) in ovarian cancer with serous, b clear cell, c endometrioid, (d) and mucinous histology. e VDR-negative control (f) and positive control in human placenta tissue. 10× (scale bar = 200 μm) and 25× (inserts, scale bar = 100 μm) magnification
From: Czogalla B, Deuster E, Liao Y, Mayr D, Schmoeckel E, Sattler C, Kolben T, Hester A, Fürst S, Burges A, Mahner S, Jeschke U, Trillsch F.
Cytoplasmic VDR expression as an independent risk factor for ovarian cancer.
Histochem Cell Biol. 2020 Oct;154(4):421-429.
doi: 10.1007/s00418-020-01894-6.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Filter by Application:
P ResetMouse anti Human Vitamin D Receptor:Preservative Free
- Product Type
- Monoclonal Antibody
- Clone
- 2F4
- Isotype
- IgG2a
- Specificity
- Vitamin D Receptor
Antibody Quality and Performance Guarantee
| Mouse anti Human Vitamin D receptor antibody, clone 2F4 recognizes the human Vitamin D3 receptor, also known as 1,25-dihydroxyvitamin D3 receptor or Nuclear receptor subfamily 1 group I member 1. The vitamin D receptor is a 427 amino acid ~ 48kDa member of the nuclear hormone receptor family containing a single nuclear receptor DNA-binding domain |
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Immunogen
- Recombinant protein corresponding to aa 1 - 428 of human Vitamin D receptor.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 |  |
0.1 | 10 ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for Vitamin D Receptor antibody
-
Ditsch, N. et al. (2012) The Association between Vitamin D Receptor Expression and Prolonged Overall Survival in Breast Cancer.
J Histochem Cytochem. 60: 121-9. -
Pulito, C. et al. (2015) Cdx2 polymorphism affects the activities of vitamin d receptor in human breast cancer cell lines and human breast carcinomas.
PLoS One. 10 (4): e0124894. -
Heublein, S. et al. (2017) Vitamin D receptor, Retinoid X receptor and peroxisome proliferator-activated receptor γ are overexpressed in BRCA1 mutated breast cancer and predict prognosis.
J Exp Clin Cancer Res. 36 (1): 57. -
Zhang, X. et al. (2017) Fluorescence Analysis of Vitamin D Receptor Status of Circulating Tumor Cells (CTCS) in Breast Cancer: From Cell Models to Metastatic Patients.
Int J Mol Sci. 18 (6)Jun 20 [Epub ahead of print]. -
Czogalla, B. et al. (2020) Cytoplasmic VDR expression as an independent risk factor for ovarian cancer.
Histochem Cell Biol. 154 (4): 421-9.
- Synonyms
- VDR
- RRID
- AB_2089282
- UniProt
- P11473
- Entrez Gene
- VDR
- GO Terms
- GO:0003700 sequence-specific DNA binding transcription factor activity
- GO:0003707 steroid hormone receptor activity
- GO:0005654 nucleoplasm
- GO:0008270 zinc ion binding
- GO:0008285 negative regulation of cell proliferation
- GO:0008434 vitamin D3 receptor activity
- GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
- GO:0010980 positive regulation of vitamin D 24-hydroxylase activity
- GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
- View More GO Terms
- GO:0046697 decidualization
- GO:0046965 retinoid X receptor binding
- GO:0060558 regulation of calcidiol 1-monooxygenase activity
MCA3543Z
145164
170320
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