Mouse anti Human Vitamin D receptor antibody, clone 2F4 recognizes the human Vitamin D3 receptor, also known as 1,25-dihydroxyvitamin D3 receptor or Nuclear receptor subfamily 1 group I member 1. The vitamin D receptor is a 427 amino acid ~ 48kDa member of the nuclear hormone receptor family containing a single nuclear receptor DNA-binding domain
- Target Species
- Product Form
- Purified IgG - liquid
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Recombinant protein corresponding to aa 1 - 428 of human Vitamin D receptor.
- Approx. Protein Concentrations
- Ig concentration 0.5 mg/ml
- Fusion Partners
- Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.
- Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
sequence-specific DNA binding transcription factor activity
steroid hormone receptor activity
zinc ion binding
negative regulation of cell proliferation
vitamin D3 receptor activity
negative regulation of gene-specific transcription from RNA polymerase II promoter
positive regulation of vitamin D 24-hydroxylase activity
regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
retinoid X receptor binding
regulation of calcidiol 1-monooxygenase activity
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Vitamin D Receptor antibody
|Immunohistology - Paraffin 1
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
Application Based External Images
Immunohistology - Paraffin
Product Specific References
References for Vitamin D Receptor antibody
Ditsch, N. et al. (2012) The Association between Vitamin D Receptor Expression and Prolonged Overall Survival in Breast Cancer.
J Histochem Cytochem. 60: 121-9.
Pulito, C. et al. (2015) Cdx2 polymorphism affects the activities of vitamin d receptor in human breast cancer cell lines and human breast carcinomas.
PLoS One. 10 (4): e0124894.
Heublein, S. et al. (2017) Vitamin D receptor, Retinoid X receptor and peroxisome proliferator-activated receptor γ are overexpressed in BRCA1 mutated breast cancer and predict prognosis.
J Exp Clin Cancer Res. 36 (1): 57.
Zhang, X. et al. (2017) Fluorescence Analysis of Vitamin D Receptor Status of Circulating Tumor Cells (CTCS) in Breast Cancer: From Cell Models to Metastatic Patients.
Int J Mol Sci. 18 (6)Jun 20 [Epub ahead of print].
Czogalla, B. et al. (2020) Cytoplasmic VDR expression as an independent risk factor for ovarian cancer.
Histochem Cell Biol. 154 (4): 421-9.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸
Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
Select the lasers and filters you wish to include
Select combined or multi-laser view to visualize the spectra