Vitamin D Receptor antibody | 2F4

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Mouse anti Human Vitamin D Receptor:Preservative Free

Product Type
Monoclonal Antibody
Clone
2F4
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA3543Z P* 0.1 mg
Mouse anti Human Vitamin D receptor antibody, clone 2F4 recognizes the human Vitamin D3 receptor, also known as 1,25-dihydroxyvitamin D3 receptor or Nuclear receptor subfamily 1 group I member 1. The vitamin D receptor is a 427 amino acid ~ 48kDa member of the nuclear hormone receptor family containing a single nuclear receptor DNA-binding domain

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 1 - 428 of human Vitamin D receptor.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P11473 Related reagents
Entrez Gene
VDR Related reagents
GO Terms
GO:0003700 sequence-specific DNA binding transcription factor activity
GO:0003707 steroid hormone receptor activity
GO:0005654 nucleoplasm
GO:0008270 zinc ion binding
GO:0008285 negative regulation of cell proliferation
GO:0008434 vitamin D3 receptor activity
GO:0010553 negative regulation of gene-specific transcription from RNA polymerase II promoter
GO:0010980 positive regulation of vitamin D 24-hydroxylase activity
GO:0034339 regulation of transcription from RNA polymerase II promoter by nuclear hormone receptor
GO:0046697 decidualization
GO:0046965 retinoid X receptor binding
GO:0060558 regulation of calcidiol 1-monooxygenase activity
Regulatory
For research purposes only

Applications of Vitamin D Receptor antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 0.1 10 ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Application Based External Images

Immunohistology - Paraffin

Product Specific References

References for Vitamin D Receptor antibody

  1. Ditsch, N. et al. (2012) The Association between Vitamin D Receptor Expression and Prolonged Overall Survival in Breast Cancer.
    J Histochem Cytochem. 60: 121-9.
  2. Pulito, C. et al. (2015) Cdx2 polymorphism affects the activities of vitamin d receptor in human breast cancer cell lines and human breast carcinomas.
    PLoS One. 10 (4): e0124894.
  3. Heublein, S. et al. (2017) Vitamin D receptor, Retinoid X receptor and peroxisome proliferator-activated receptor γ are overexpressed in BRCA1 mutated breast cancer and predict prognosis.
    J Exp Clin Cancer Res. 36 (1): 57.
  4. Zhang, X. et al. (2017) Fluorescence Analysis of Vitamin D Receptor Status of Circulating Tumor Cells (CTCS) in Breast Cancer: From Cell Models to Metastatic Patients.
    Int J Mol Sci. 18 (6)Jun 20 [Epub ahead of print].