Mouse anti Human visfatin antibody recognizes human visfatin also known as nicotinamide phosphoribosyltransferase and pre-B-cell colony-enhancing factor 1, a putative cytokine and member of the NAPRTase family involved in catalyzing the biosynthesis of NAD from nicotinamide.

Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T- and B-cells, dendritic cells and macrophages, suggesting a role in inflammatory processes. Visfatin is produced by visceral fat and appears to have effects similar to those of insulin (Fukuhara et al. 2005). Plasma levels of visfatin are elevated in patients with type 2 diabetes mellitus (Chen et al. 2006). It has also been suggested that macrophages in visceral white adipose tissue may be a prominent source of the adipokines visfatin and resistin as well as chemokines MCP-1, MIP1α and IL-8, suggesting a pro-inflammatory marker role for visfatin (Curat et al. 2006).

Mouse anti Human Visfatin antibody recognizes human visfatin as a single band of ~56 kDa in K562 cell lysates and in other human cell line lysates by western blotting under reducing conditions. The antibody also cross-reacts with visfatin from mouse and rat cell lines.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting			1/1000