Visfatin antibody
Mouse anti Visfatin
Visfatin antibody recognizes nicotinamide phosphoribosyltransferase, also known as NAmPRTase, Nampt, pre-B-cell colony-enhancing factor 1, pre-B cell-enhancing factor or visfatin. The antibody has been extensively validated for western blotting using whole cell lysates.
Visfatin Antibody with Control Lysate
Visfatin antibody recognizes nicotinamide phosphoribosyltransferase, also known as NAmPRTase, Nampt, pre-B-cell colony-enhancing factor 1, pre-B cell-enhancing factor or visfatin. The antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00043 | WB | 100 µl | ||||
| VMA00043KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human visfatin antibody recognizes human visfatin also known as nicotinamide phosphoribosyltransferase and pre-B-cell colony-enhancing factor 1, a putative cytokine and member of the NAPRTase family involved in catalyzing the biosynthesis of NAD from nicotinamide.
Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T- and B-cells, dendritic cells and macrophages, suggesting a role in inflammatory processes. Visfatin is produced by visceral fat and appears to have effects similar to those of insulin (Fukuhara et al. 2005). Plasma levels of visfatin are elevated in patients with type 2 diabetes mellitus (Chen et al. 2006). It has also been suggested that macrophages in visceral white adipose tissue may be a prominent source of the adipokines visfatin and resistin as well as chemokines MCP-1, MIP1α and IL-8, suggesting a pro-inflammatory marker role for visfatin (Curat et al. 2006).
Mouse anti Human Visfatin antibody recognizes human visfatin as a single band of ~56 kDa in K562 cell lysates and in other human cell line lysates by western blotting under reducing conditions. The antibody also cross-reacts with visfatin from mouse and rat cell lines.
Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T- and B-cells, dendritic cells and macrophages, suggesting a role in inflammatory processes. Visfatin is produced by visceral fat and appears to have effects similar to those of insulin (Fukuhara et al. 2005). Plasma levels of visfatin are elevated in patients with type 2 diabetes mellitus (Chen et al. 2006). It has also been suggested that macrophages in visceral white adipose tissue may be a prominent source of the adipokines visfatin and resistin as well as chemokines MCP-1, MIP1α and IL-8, suggesting a pro-inflammatory marker role for visfatin (Curat et al. 2006).
Mouse anti Human Visfatin antibody recognizes human visfatin as a single band of ~56 kDa in K562 cell lysates and in other human cell line lysates by western blotting under reducing conditions. The antibody also cross-reacts with visfatin from mouse and rat cell lines.
Product Details
- Target Species
- Human
- Western Blotting
- Anti visfatin antibody detects a band of approximately 56 kDa in K562 cell lysates.
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant human visfatin, amino acids 1-491, purified from E. coli
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg K562 lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Guarantee
- As supplied, 12 months from date of despatch.
- Guarantee
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P43490 Related reagents
- Entrez Gene
- NAMPT Related reagents
- GO Terms
- GO:0004514 nicotinate-nucleotide diphosphorylase (carboxylating) activity
- GO:0005829 cytosol
- GO:0007165 signal transduction
- GO:0005125 cytokine activity
- GO:0006769 nicotinamide metabolic process
- GO:0007267 cell-cell signaling
- GO:0008284 positive regulation of cell proliferation
- GO:0009435 NAD biosynthetic process
- GO:0047280 nicotinamide phosphoribosyltransferase activity
- GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of Visfatin antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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