Visfatin antibody | 4D5

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Anti Visfatin Antibody, clone 4D5 (PrecisionAb Monoclonal Antibody) thumbnail image 1

Anti Visfatin Antibody, clone 4D5 (PrecisionAb Monoclonal Antibody) thumbnail image 2

Anti Visfatin Antibody, clone 4D5 (PrecisionAb Monoclonal Antibody) thumbnail image 3

Anti Visfatin Antibody, clone 4D5 (PrecisionAb Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with visfatin antibody (**VMA00043**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 300 second exposure. Arrow points to visfatin (molecular weight 56 kDa).

Anti Visfatin Antibody, clone 4D5 (PrecisionAb Monoclonal Antibody) gallery image 2 — Western blot analysis of whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Visfatin (1 & 2) or a scrambled siRNA (-) probed with Mouse anti Visfatin antibody (**VMA00043**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000 STAR207P) and hFAB rhodamine anti-actin primary antibody (12004164) and visualized on the ChemiDoc MP with an 149 second (chemiluminescent channel) and 30 second (rhodamine channel) exposure.

Anti Visfatin Antibody, clone 4D5 (PrecisionAb Monoclonal Antibody) gallery image 3 — Normalized signal intensity of Visfatin from whole cell lysates prepared from HEK293 separately transfected with two unique siRNA duplexes against Visfatin (1 & 2) or a scrambled siRNA (-). Signal intensity was normalized to the actin signal.

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Mouse anti Visfatin

Product Type: PrecisionAb Monoclonal
Clone: 4D5
Isotype: IgG1
Format: Purified
Specificity: Visfatin

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Secondary Antibodies
References (1)
Applications
Storage Information
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
VMA00043 Datasheet Datasheet SDS SDS	E WB	100 µl		Log in	Get Quote
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Mouse anti Human visfatin antibody recognizes human visfatin also known as nicotinamide phosphoribosyltransferase and pre-B-cell colony-enhancing factor 1, a putative cytokine and member of the NAPRTase family involved in catalyzing the biosynthesis of NAD from nicotinamide.

Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T- and B-cells, dendritic cells and macrophages, suggesting a role in inflammatory processes. Visfatin is produced by visceral fat and appears to have effects similar to those of insulin (Fukuhara et al. 2005). Plasma levels of visfatin are elevated in patients with type 2 diabetes mellitus (Chen et al. 2006). It has also been suggested that macrophages in visceral white adipose tissue may be a prominent source of the adipokines visfatin and resistin as well as chemokines MCP-1, MIP1α and IL-8, suggesting a pro-inflammatory marker role for visfatin (Curat et al. 2006).

Mouse anti Human Visfatin antibody recognizes human visfatin as a single band of ~56 kDa in K562 cell lysates and in other human cell line lysates by western blotting under reducing conditions. The antibody also cross-reacts with visfatin from mouse and rat cell lines.

Target Species: Human
Western Blotting: Anti visfatin antibody detects a band of approximately 56 kDa in K562 cell lysates.
Species Cross-Reactivity: Target Species Cross Reactivity

Mouse

Rat; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution: Phosphate buffered saline.
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃).
Immunogen: Recombinant human visfatin, amino acids 1-491, purified from E. coli
Approx. Protein Concentrations: 0.5 mg/ml
Regulatory: For research purposes only.
Guarantee: 12 months from date of despatch.
Acknowledgements: PrecisionAb is a trademark of Bio-Rad Laboratories.

Target Species	Cross Reactivity
Mouse
Rat

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
ELISA
Western Blotting			1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description	Product Code	Applications	Pack Size	Your Price
Goat anti Mouse IgG (H/L):HRP	STAR207P	WB	2 ml	Log in	Get Quote
List Price	Your Price
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Description	Goat anti Mouse IgG (H/L):HRP

References for Visfatin antibody

Bułdak, R.J. et al. (2014) Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment.
Eur J Histochem. 58 (3): 2408.

Synonyms: NAMPT
UniProt: P43490
Entrez Gene: NAMPT
GO Terms: GO:0004514 nicotinate-nucleotide diphosphorylase (carboxylating) activity; GO:0005829 cytosol; GO:0007165 signal transduction; GO:0005125 cytokine activity; GO:0006769 nicotinamide metabolic process; GO:0007267 cell-cell signaling; GO:0008284 positive regulation of cell proliferation; GO:0009435 NAD biosynthetic process; GO:0047280 nicotinamide phosphoribosyltransferase activity; View More GO Terms; GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process