Visfatin antibody | 4D5

Mouse anti Visfatin

Product Type
PrecisionAb Monoclonal

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SDS Safety Datasheet SDS
E WB 100 µl loader
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Mouse anti Human visfatin antibody recognizes human visfatin also known as nicotinamide phosphoribosyltransferase and pre-B-cell colony-enhancing factor 1, a putative cytokine and member of the NAPRTase family involved in catalyzing the biosynthesis of NAD from nicotinamide.

Visfatin is highly expressed in bone marrow, muscle and liver tissue and increases in expression in response to endotoxin, in amniotic epithelial cells following lipopolysaccharide (LPS) stimulation, and during the activation of T- and B-cells, dendritic cells and macrophages, suggesting a role in inflammatory processes. Visfatin is produced by visceral fat and appears to have effects similar to those of insulin (Fukuhara et al. 2005). Plasma levels of visfatin are elevated in patients with type 2 diabetes mellitus (Chen et al. 2006). It has also been suggested that macrophages in visceral white adipose tissue may be a prominent source of the adipokines visfatin and resistin as well as chemokines MCP-1, MIP1α and IL-8, suggesting a pro-inflammatory marker role for visfatin (Curat et al. 2006).

Mouse anti Human Visfatin antibody recognizes human visfatin as a single band of ~56 kDa in K562 cell lysates and in other human cell line lysates by western blotting under reducing conditions. The antibody also cross-reacts with visfatin from mouse and rat cell lines.
PrecisionAb Antibodies

Target Species
Western Blotting
Anti visfatin antibody detects a band of approximately 56 kDa in K562 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Mouse monoclonal antibody purified by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Recombinant human visfatin, amino acids 1-491, purified from E. coli
Approx. Protein Concentrations
0.5 mg/ml
For research purposes only.
12 months from date of despatch.
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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References for Visfatin antibody

  1. Bułdak, R.J. et al. (2014) Changes in subcellular localization of visfatin in human colorectal HCT-116 carcinoma cell line after cytochalasin B treatment.
    Eur J Histochem. 58 (3): 2408.

Entrez Gene
GO Terms
GO:0004514 nicotinate-nucleotide diphosphorylase (carboxylating) activity
GO:0005829 cytosol
GO:0007165 signal transduction
GO:0005125 cytokine activity
GO:0006769 nicotinamide metabolic process
GO:0007267 cell-cell signaling
GO:0008284 positive regulation of cell proliferation
GO:0009435 NAD biosynthetic process
GO:0047280 nicotinamide phosphoribosyltransferase activity
GO:0051770 positive regulation of nitric-oxide synthase biosynthetic process



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