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Vinculin antibody | F01/4H8

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Anti Vinculin Antibody, clone F01/4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 1 Anti Vinculin Antibody, clone F01/4H8 (PrecisionAb Monoclonal Antibody) thumbnail image 2
Anti Vinculin Antibody, clone F01/4H8 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with vinculin antibody (1/1000, VMA00895) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, STAR207P) and visualized on the ChemiDoc MP. Arrow points to vinculin (molecular weight 130 kDa)
Anti Vinculin Antibody, clone F01/4H8 (PrecisionAb Monoclonal Antibody) gallery image 2
Immunoprecipitation of vinculin from HEK293 whole cell lysate. The Input lane is 10% input; the VCL-IP lane is the immunoprecipitation with the vinculin antibody (2.5 µg, VMA00895); the IgG-IP lane is the immunoprecipitation with Mouse monoclonal IgG (2.5 µg, MCA928). Western blot analysis was performed using the vinculin antibody (1/1000, VMA00895) followed by detection with HRP conjugated Goat anti Mouse IgG (1/20,000, 1705047) and visualized on the ChemiDoc MP with 26 second exposure. Arrow points to vinculin.

Mouse anti Vinculin

Product Type
PrecisionAb Monoclonal
Clone
F01/4H8
Isotype
IgG1
Format
Purified
Specificity
Vinculin

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Product Code Applications Pack Size List Price Your Price Qty
VMA00895
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IP WB 100 µl loader
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Mouse anti vinculin antibody recognizes vinculin, also known as metavinculin.

Vinculin is a non-ezymatic, cytoplasmic, actin-binding protein that regulates adhesion by stimulating actin polymerization and recruiting actin remodeling proteins. Cell-matrix and cell-cell adhesion are dramatically impaired in the absence of vinculin, revealing its essential role (Bays and DeMali 2017). Vinculin can regulate cell migration, a process determined by cell adhesion and the turnover of focal adhesions. The protein is also required for maintaining integrity of glomerular barriers in the kidney (Lausecker et al. 2018). Downregulation of vinculin has been identified in metastatic cancer cells, and loss of vinculin appears to protect cells from apoptosis (Gao et al. 2017). Therefore, vinculin appears to act as a tumor suppressor and affects tumorigenesis, metastasis, and invasion. In non-small cell lung cancer, vinculin expression is inhibited and this promotes malignancy (Yu et al. 2020). 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti vinculin antibody detects a band of approximately 130 kDa in HEK293 cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - Liquid
Preparation
Mouse monoclonal antibody affinity purified on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide
Immunogen
E. coli-derived recombinant protein of amino acids 1-1135 of human vinculin
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the mouse SP2/0 myeloma cell line
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20°C, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunoprecipitation
Western Blotting 1/1000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P18206
Entrez Gene
VCL
GO Terms
GO:0002009 morphogenesis of an epithelium
GO:0002166 beta-dystroglycan binding
GO:0002576 platelet degranulation
GO:0006936 muscle contraction
GO:0003779 actin binding
GO:0005576 extracellular region
GO:0005829 cytosol
GO:0005198 structural molecule activity
GO:0005925 focal adhesion
GO:0006928 cellular component movement
GO:0007160 cell-matrix adhesion
GO:0008013 beta-catenin binding
GO:0030032 lamellipodium assembly
GO:0030168 platelet activation
GO:0030336 negative regulation of cell migration
GO:0034333 adherens junction assembly
GO:0034394 protein localization at cell surface
GO:0043034 costamere
GO:0043297 apical junction assembly
GO:0045294 alpha-catenin binding
GO:0045296 cadherin binding
GO:0090136 epithelial cell-cell adhesion

VMA00895

100004256 100006534 64595330

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