Vesicle-fusing ATPase antibody | 9G7-3
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WB ResetMouse anti Vesicle-fusing ATPase
- Product Type
- Monoclonal Antibody
- Clone
- 9G7-3
- Isotype
- IgG2b
- Specificity
- Vesicle-fusing ATPase
Antibody Quality and Performance Guarantee
| Mouse anti vesicle-fusing ATPase antibody, clone 9G7-3 recognizes vesicle-fusing ATPase also known as NEM-sensitive fusion protein or NSF. Vesicle-fusing ATPase is a member of the hydrolase protein family, which play an essential role in vesicle-mediated transport. This enzyme acts within the Golgi cisternae by catalyzing the fusion of transport vesicles as well as delivering cargo proteins to all Golgi compartments. Vesicle-fusing ATPase is also essential for facilitating transport from the endoplasmic reticulum to the Golgi body itself. |
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Bovine Hamster Monkey Pig Mouse Rabbit Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from ascites
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Recombinant bovine vesicle-fusing ATPase
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the murine NS-1 myeloma.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin |  |
||
| Western Blotting | |
1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse anti vesicle-fusing ATPase recognizes a band of ~76 kDa by western blot
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml | Log in | |||
| List Price | Your Price | ||||||
| Log in | |||||||
| Description | TidyBlot Western Blot Detection Reagent:HRP | ||||||
Source Reference
-
Steegmaier, M. et al. (1998) Three novel proteins of the syntaxin/SNAP-25 family.
J Biol Chem. 273 (51): 34171-9.
- Synonyms
- NSF
- UniProt
- P46459
- Entrez Gene
- NSF
- GO Terms
- GO:0004252 serine-type endopeptidase activity
- GO:0004176 ATP-dependent peptidase activity
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006508 proteolysis
- GO:0007268 synaptic transmission
- GO:0015031 protein transport
MCA6038
154649
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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