Vesicle-fusing ATPase antibody | 9G7-3

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Anti Vesicle-fusing ATPase Antibody, clone 9G7-3 gallery image 1 — Western blot analysis of HeLa (heat shocked; lane 2), Vero (lane 3), CHO-K1 (lane 4) and ESK-4 (lane 5) cell lysates probed with Mouse anti vesicle-fusing ATPase antibody, clone 9G7-3 (**MCA6038**). Molecular weight marker is shown in lane 1.

Mouse anti Vesicle-fusing ATPase

Product Type: Monoclonal Antibody
Clone: 9G7-3
Isotype: IgG2b

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
MCA6038	P WB			50 µg

Mouse anti vesicle-fusing ATPase antibody, clone 9G7-3 recognizes vesicle-fusing ATPase also known as NEM-sensitive fusion protein or NSF.

Vesicle-fusing ATPase is a member of the hydrolase protein family, which play an essential role in vesicle-mediated transport. This enzyme acts within the Golgi cisternae by catalyzing the fusion of transport vesicles as well as delivering cargo proteins to all Golgi compartments. Vesicle-fusing ATPase is also essential for facilitating transport from the endoplasmic reticulum to the Golgi body itself.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat
Bovine
Hamster
Monkey
Pig
Mouse
Rabbit
Sheep

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Purified IgG prepared by affinity chromatography on Protein G

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
50% Glycerol

Immunogen

Recombinant bovine vesicle-fusing ATPase

Approx. Protein Concentrations

IgG concentration 1.0 mg/ml

Fusion Partners

Spleen cells from immunized Balb/c mice were fused with cells of the murine NS-1 myeloma.

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P46459 Related reagents
Entrez Gene: NSF Related reagents
GO Terms: GO:0004252 serine-type endopeptidase activity; GO:0004176 ATP-dependent peptidase activity; GO:0005829 cytosol; GO:0046872 metal ion binding; GO:0005524 ATP binding; GO:0006508 proteolysis; GO:0007268 synaptic transmission; GO:0015031 protein transport
Regulatory: For research purposes only

Applications of Vesicle-fusing ATPase antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Immunohistology - Paraffin
Western Blotting			1/1000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Mouse anti vesicle-fusing ATPase recognizes a band of ~76 kDa by western blot

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications
Human anti Mouse IgG2b:FITC	HCA038F	0.1 mg	F
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed)	STAR117A	0.5 mg	E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed)	STAR117D488GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed)	STAR117D549GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed)	STAR117D649GA	0.1 mg	F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed)	STAR117D680GA	0.1 mg	F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed)	STAR117D800GA	0.1 mg	F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed)	STAR117F	0.5 mg	F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed)	STAR117P	0.5 mg	E WB
Goat anti Mouse IgG (Fc):FITC	STAR120F	1 mg	C F
Goat anti Mouse IgG (Fc):HRP	STAR120P	1 mg	E WB
Rabbit F(ab')2 anti Mouse IgG:RPE	STAR12A	1 ml	F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed)	STAR13B	1 mg	C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed)	STAR70	0.5 mg	F
Goat anti Mouse IgG:RPE (Rat Adsorbed)	STAR76	1 ml	F
Goat anti Mouse IgG:HRP (Rat Adsorbed)	STAR77	0.5 mg	C E P
Goat anti Mouse IgG/A/M:Alk. Phos.	STAR87A	1 mg	C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed)	STAR87P	1 mg	E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800	STAR8D800GA	0.1 mg	F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC	STAR9B	1 mg	F

Useful Reagents Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	0.5 ml	WB*

Product Specific References

Source Reference

Steegmaier, M. et al. (1998) Three novel proteins of the syntaxin/SNAP-25 family.
J Biol Chem. 273 (51): 34171-9.

Request a different product with the Vesicle-fusing ATPase specificity