Vesicle-fusing ATPase antibody | 9G7-3
Mouse anti vesicle-fusing ATPase antibody, clone 9G7-3 recognizes vesicle-fusing ATPase also known as NEM-sensitive fusion protein or NSF.
Vesicle-fusing ATPase is a member of the hydrolase protein family, which play an essential role in vesicle-mediated transport. This enzyme acts within the Golgi cisternae by catalyzing the fusion of transport vesicles as well as delivering cargo proteins to all Golgi compartments. Vesicle-fusing ATPase is also essential for facilitating transport from the endoplasmic reticulum to the Golgi body itself.
Vesicle-fusing ATPase is a member of the hydrolase protein family, which play an essential role in vesicle-mediated transport. This enzyme acts within the Golgi cisternae by catalyzing the fusion of transport vesicles as well as delivering cargo proteins to all Golgi compartments. Vesicle-fusing ATPase is also essential for facilitating transport from the endoplasmic reticulum to the Golgi body itself.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Bovine Hamster Monkey Pig Mouse Rabbit Sheep - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
50% Glycerol - Immunogen
- Recombinant bovine vesicle-fusing ATPase
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with cells of the murine NS-1 myeloma.
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P46459
- Entrez Gene
- NSF
- GO Terms
- GO:0004252 serine-type endopeptidase activity
- GO:0004176 ATP-dependent peptidase activity
- GO:0005829 cytosol
- GO:0046872 metal ion binding
- GO:0005524 ATP binding
- GO:0006508 proteolysis
- GO:0007268 synaptic transmission
- GO:0015031 protein transport
- Regulatory
- For research purposes only
Applications of Vesicle-fusing ATPase antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin | |||
| Western Blotting | 1/1000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Mouse anti vesicle-fusing ATPase recognizes a band of ~76 kDa by western blot
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Secondary Antibodies Available
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Product Specific References
Source Reference
-
Steegmaier, M. et al. (1998) Three novel proteins of the syntaxin/SNAP-25 family.
J Biol Chem. 273 (51): 34171-9.
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