VEGF Receptor 3 antibody | 5B5

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Mouse anti Human VEGF Receptor 3:Preservative Free

Product Type
Monoclonal Antibody
Clone
5B5
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA5331Z WB 0.1 mg
Mouse anti Human VEGF receptor 3 antibody, clone 5B5 recognizes human vascular endothelial growth factor receptor 3, also known as Fms-like tyrosine kinase 4, FLT-4 or Tyrosine-protein kinase receptor FLT4. FLT-4 is a 1339 amino acid ~160 kDa single pass type 1 transmembrane glycoprotein receptor for VEGFC and VEGFD, playing an essential role in adult lymphangiogenesis.

Three isoforms produced by alternative splicing have been described. Mouse anti Human VEGF receptor 3 antibody, clone 5B5 binds to the canonical isoform 1, isoforms 2 and 3 also preserve the complete unaltered immunogen sequence used to generate clone 5B5 and therefore also be expected to be recognized by the antibody.

Mutations of the FLT4 gene can lead to Lymphedema, hereditary, 1A (LMPH1A), a chronic condition with swelling of the extremities caused by alterations in lymph flow (Irrthum et al. 2000). Mutations can also lead to increases susceptibility to Hemangioma, capillary infantile (HCI), a condition characterised by sharply demarcated skin hemangiomas present at birth or within the first few months after birth as a result of highly proliferative capillary endothelial growth. These generally undergo regression and involution (Walter et al. 2002).

Product Details

Target Species
Human
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Immunogen
Recombinant protein corresponding to aa 34-133 of human VEGF receptor 3.
Approx. Protein Concentrations
Ig concentration 0.5 mg/ml
Fusion Partners
Spleen cells from BALB/c mice were fused with cells from the Sp2/0 myeloma cell line.

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P35916 Related reagents
Entrez Gene
FLT4 Related reagents
GO Terms
GO:0005515 protein binding
GO:0005021 vascular endothelial growth factor receptor activity
GO:0005887 integral to plasma membrane
GO:0005524 ATP binding
GO:0006468 protein phosphorylation
GO:0008284 positive regulation of cell proliferation
Regulatory
For research purposes only

Applications of VEGF Receptor 3 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 0.1 10 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Human anti Mouse IgG2a:FITC HCA037F 0.1 mg F
Human anti Mouse IgG2a:HRP HCA037P 0.1 mg E
Goat anti Mouse IgG (H/L):Alk. Phos. (Multi Species Adsorbed) STAR117A 0.5 mg E WB
Goat anti Mouse IgG (H/L):DyLight®488 (Multi Species Adsorbed) STAR117D488GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®549 (Multi Species Adsorbed) STAR117D549GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):DyLight®649 (Multi Species Adsorbed) STAR117D649GA 0.1 mg F IF
Goat anti Mouse IgG (H/L):DyLight®680 (Multi Species Adsorbed) STAR117D680GA 0.1 mg F WB
Goat anti Mouse IgG (H/L):DyLight®800 (Multi Species Adsorbed) STAR117D800GA 0.1 mg F IF WB
Goat anti Mouse IgG (H/L):FITC (Multi Species Adsorbed) STAR117F 0.5 mg F
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Goat anti Mouse IgG (Fc):FITC STAR120F 1 mg C F
Goat anti Mouse IgG (Fc):HRP STAR120P 1 mg E WB
Rabbit F(ab')2 anti Mouse IgG:RPE STAR12A 1 ml F
Rabbit F(ab')2 anti Mouse IgG:HRP (Human Adsorbed) STAR13B 1 mg C E P RE WB
Goat anti Mouse IgG:FITC (Rat Adsorbed) STAR70 0.5 mg F
Goat anti Mouse IgG:RPE (Rat Adsorbed) STAR76 1 ml F
Goat anti Mouse IgG:HRP (Rat Adsorbed) STAR77 0.5 mg C E P
Goat anti Mouse IgG/A/M:Alk. Phos. STAR87A 1 mg C E WB
Goat anti Mouse IgG/A/M:HRP (Human Adsorbed) STAR87P 1 mg E
Rabbit F(ab')2 anti Mouse IgG:Dylight®800 STAR8D800GA 0.1 mg F IF WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F