USP9X antibody | OTI1D7

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Mouse anti USP9X

USP9X antibody recognizes the deubiquitinase ubiquitin-specific peptidase 9 X linked, also known as carboxyl-terminal hydrolase FAF-x, Drosophila fat facets related X-linked or deubiquitinating enzyme FAF-X. This antibody has been extensively validated for western blotting using whole cell lysates.

USP9X Antibody with Control Lysate

USP9X antibody recognizes the deubiquitinase ubiquitin-specific peptidase 9 X linked, also known as carboxyl-terminal hydrolase FAF-x, Drosophila fat facets related X-linked or deubiquitinating enzyme FAF-X. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type
PrecisionAb™ Monoclonal
Clone
OTI1D7
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00191 WB 100 µl
VMA00191KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human USP9X antibody recognizes USP9X, also known as Drosophila fat facets related, X-linked, deubiquitinating enzyme FAF-X, fat facets in mammals, fat facets protein related, X-linked, fat facets protein-related, X-linked, hFAM, probable ubiquitin carboxyl-terminal hydrolase FAF-X, ubiquitin specific protease 9, X chromosome (fat facets-like Drosophila), ubiquitin thioesterase FAF-X, ubiquitin thiolesterase FAF-X, ubiquitin-specific processing protease FAF-X, ubiquitin-specific protease 9, X chromosome and ubiquitin-specific-processing protease FAF-X.

USP9X is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though USP9X is located on the X chromosome, it escapes X-inactivation. Mutations in USP9X have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human USP9X antibody detects a band of 270 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti USP9X detects a band of approximately 270 kDa in HEK293 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation
20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Recombinant protein corresponding to amino acids 2246-2570 of human USP9X (NP_001034680) produced in E. coli
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
Q93008 Related reagents
Entrez Gene
USP9X Related reagents
GO Terms
GO:0007059 chromosome segregation
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0005737 cytoplasm
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0007067 mitosis
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007292 female gamete generation
GO:0016579 protein deubiquitination
GO:0030509 BMP signaling pathway
GO:0051301 cell division
GO:0070410 co-SMAD binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of USP9X antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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