USP7 antibody | OTI1F12
Mouse anti USP7
USP7 antibody recognizes ubiquitin carboxyl-terminal hydrolase 7, also known as herpes virus-associated ubiquitin-specific protease, deubiquitinating enzyme 7 or ubiquitin thioesterase 7. This antibody has been extensively validated for western blotting using whole cell lysates.
USP7 Antibody with Control Lysate
USP7 antibody recognizes ubiquitin carboxyl-terminal hydrolase 7, also known as herpes virus-associated ubiquitin-specific protease, deubiquitinating enzyme 7 or ubiquitin thioesterase 7. This antibody has been extensively validated for western blotting using whole cell lysates.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI1F12
- Isotype
- IgG1
- Format
- Purified
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00216 | WB | 100 µl | ||||
| VMA00216KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human USP7 antibody recognizes the ubiquitin carboxyl-terminal hydrolase 7, also known as Herpes virus-associated ubiquitin-specific protease, deubiquitinating enzyme 7, herpesvirus-associated ubiquitin-specific protease, ubiquitin carboxyl-terminal hydrolase 7, ubiquitin specific protease 7 (herpes virus-associated), ubiquitin thioesterase 7 and ubiquitin-specific-processing protease 7.
Mouse anti Human USP7 antibody detects a band of 128 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Mouse anti Human USP7 antibody detects a band of 128 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Western Blotting
- Anti USP7 detects a band of approximately 128 kDa in Jurkat cell lysates
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human USP7 (NP_003461) produced in HEK293T cells
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
Positive Control Lysate
- Lysate Composition
- 400μg Jurkat lysate lyophilized in RIPA buffer.
- Lysate Reconstitution
- - If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- Q93009 Related reagents
- Entrez Gene
- USP7 Related reagents
- GO Terms
- GO:0004197 cysteine-type endopeptidase activity
- GO:0002039 p53 binding
- GO:0004221 ubiquitin thiolesterase activity
- GO:0004843 ubiquitin-specific protease activity
- GO:0005737 cytoplasm
- GO:0006511 ubiquitin-dependent protein catabolic process
- GO:0007275 multicellular organismal development
- GO:0008022 protein C-terminus binding
- GO:0008134 transcription factor binding
- GO:0016579 protein deubiquitination
- GO:0016605 PML body
- GO:0031625 ubiquitin protein ligase binding
- GO:0044419 interspecies interaction between organisms
- GO:0051090 regulation of transcription factor activity
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of USP7 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
Copyright © 2018 Bio-Rad Antibodies (formerly AbD Serotec)
Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
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