USP7 antibody | OTI1F12

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Anti USP7 Antibody, clone OTI1F12 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with USP7 antibody (**VMA00216**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 299 second exposure. Arrow points to USP7 (molecular weight 128 kDa).

Mouse anti USP7

USP7 antibody recognizes ubiquitin carboxyl-terminal hydrolase 7, also known as herpes virus-associated ubiquitin-specific protease, deubiquitinating enzyme 7 or ubiquitin thioesterase 7. This antibody has been extensively validated for western blotting using whole cell lysates.

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1F12
Isotype: IgG1
Format: Purified

Product Code	Applications	Datasheet	MSDS	Pack Size	List Price	Quantity
VMA00216	WB			100 µl

Summary
Secondary Antibodies

Mouse anti Human USP7 antibody recognizes the ubiquitin carboxyl-terminal hydrolase 7, also known as Herpes virus-associated ubiquitin-specific protease, deubiquitinating enzyme 7, herpesvirus-associated ubiquitin-specific protease, ubiquitin carboxyl-terminal hydrolase 7, ubiquitin specific protease 7 (herpes virus-associated), ubiquitin thioesterase 7 and ubiquitin-specific-processing protease 7.

Mouse anti Human USP7 antibody detects a band of 128 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti USP7 detects a band of approximately 128 kDa in Jurkat cell lysates

Species Cross-Reactivity

Target Species	Cross Reactivity
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from ascites

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant human USP7 (NP_003461) produced in HEK293T cells

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Guarantee: As supplied, 12 months from date of despatch.

More Information

UniProt: Q93009 Related reagents
Entrez Gene: USP7 Related reagents
GO Terms: GO:0004197 cysteine-type endopeptidase activity; GO:0002039 p53 binding; GO:0004221 ubiquitin thiolesterase activity; GO:0004843 ubiquitin-specific protease activity; GO:0005737 cytoplasm; GO:0006511 ubiquitin-dependent protein catabolic process; GO:0007275 multicellular organismal development; GO:0008022 protein C-terminus binding; GO:0008134 transcription factor binding; GO:0016579 protein deubiquitination; GO:0016605 PML body; GO:0031625 ubiquitin protein ligase binding; GO:0044419 interspecies interaction between organisms; GO:0051090 regulation of transcription factor activity
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of USP7 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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