USP5 antibody | OTI1F8

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Anti USP5 Antibody, clone OTI1F8 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with USP5 antibody (**VMA00135**) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc™ MP with 6 second exposure. Arrow points to USP5 (molecular weight 105 kDa).

Mouse anti USP5

USP5 antibody recognizes ubiquitin carboxyl-terminal hydrolase 5, also known as deubiquitinating enzyme 5, isopeptidase T, ubiquitin isopeptidase T, ubiquitin thioesterase 5 or ubiquitin-specific protease-5. This antibody has been extensively validated for western blotting using whole cell lysates.

USP5 Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI1F8
Isotype: IgG2a
Format: Purified

Product Code	Applications	Pack Size
VMA00135	WB	100 µl
VMA00135KT	WB	2 Westerns
With Lysate		400µg

Mouse anti Human USP5 antibody recognizes USP5, also known as deubiquitinating enzyme 5, isopeptidase T, ubiquitin carboxyl-terminal hydrolase 5, ubiquitin isopeptidase T, ubiquitin specific protease 5 (isopeptidase T), ubiquitin thioesterase 5, ubiquitin thiolesterase 5, ubiquitin-specific protease-5 (ubiquitin isopeptidase T) and ubiquitin-specific-processing protease 5.

Ubiquitin dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al. 1995).

Mouse anti Human USP5 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species

Human

Western Blotting

Anti USP5 detects a band of approximately 105 kDa in MCF7 cell lysates.

Species Cross-Reactivity

Target Species	Cross Reactivity
Mouse
Rat

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Purified IgG - liquid

Product Form

Purified IgG - liquid

Preparation

Mouse monoclonal antibody purified by affinity chromatography from ascites.

Preparation

20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.

Buffer Solution

Phosphate buffered saline

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Preservative Stabilisers

0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol

Immunogen

Full length recombinant human USP5 (NP_003472) produced in HEK293T cells

Instructions For Use

Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg MCF7 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P45974 Related reagents
Entrez Gene: USP5 Related reagents
GO Terms: GO:0005515 protein binding; GO:0004197 cysteine-type endopeptidase activity; GO:0005764 lysosome; GO:0004221 ubiquitin thiolesterase activity; GO:0006511 ubiquitin-dependent protein catabolic process; GO:0008270 zinc ion binding; GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process; GO:0071108 protein K48-linked deubiquitination
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of USP5 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

Product Specific References

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