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USP5 antibody | OTI1F8

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Anti USP5 Antibody, clone OTI1F8 (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti USP5 Antibody, clone OTI1F8 (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with Mouse anti Human USP5 antibody, clone OTI1F8 (VMA00135) followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 6 second exposure. Arrow points to USP5 (molecular weight 105 kDa).

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WB

Mouse anti USP5

Product Type
PrecisionAb Monoclonal
Clone
OTI1F8
Isotype
IgG2a
Format
Purified
Specificity
USP5

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Technical Suppport
Product Code Applications Pack Size List Price Your Price Qty
VMA00135
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SDS Safety Datasheet SDS
WB 100 µl loader
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loader
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Mouse anti Human USP5 antibody, clone OTI1F8 recognizes USP5, also known as deubiquitinating enzyme 5, isopeptidase T, ubiquitin carboxyl-terminal hydrolase 5, ubiquitin isopeptidase T, ubiquitin specific protease 5 (isopeptidase T), ubiquitin thioesterase 5, ubiquitin thiolesterase 5, ubiquitin-specific protease-5 (ubiquitin isopeptidase T) and ubiquitin-specific-processing protease 5.

Ubiquitin dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al. 1995).

Mouse anti Human USP5 antibody detects a band of 105 kDa. The antibody has been extensively validated for western blotting using whole cell lysates. 		PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti USP5 detects a band of approximately 105 kDa in MCF7 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human USP5 (NP_003472) produced in HEK293T cells
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
List Price Your Price
loader
Description Goat anti Mouse IgG (H/L):HRP

Further Reading

  1. Wilkinson KD et al. (1995) Metabolism of the polyubiquitin degradation signal: structure, mechanism, and role of isopeptidase T.
    Biochemistry. 34 (44): 14535-46.

UniProt
P45974
Entrez Gene
USP5
GO Terms
GO:0005515 protein binding
GO:0004197 cysteine-type endopeptidase activity
GO:0005764 lysosome
GO:0004221 ubiquitin thiolesterase activity
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0008270 zinc ion binding
GO:0032436 positive regulation of proteasomal ubiquitin-dependent protein catabolic process
GO:0071108 protein K48-linked deubiquitination
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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