USP14 antibody

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Mouse anti USP14

USP14 antibody recognizes ubiquitin carboxyl-terminal hydrolase 14, also known as deubiquitinating enzyme 14, tRNA-guanine transglycosylase, 60-kDa subunit, ubiquitin specific protease 14 (tRNA-guanine transglycosylase), or ubiquitin thioesterase 14.

USP14 Antibody with Control Lysate

USP14 antibody recognizes ubiquitin carboxyl-terminal hydrolase 14, also known as deubiquitinating enzyme 14, tRNA-guanine transglycosylase, 60-kDa subunit, ubiquitin specific protease 14 (tRNA-guanine transglycosylase), or ubiquitin thioesterase 14.

Product Type
PrecisionAb™ Monoclonal
Isotype
IgG1
Format
Purified

Select your package type:

Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
VMA00300 WB 100 µl
VMA00300KT WB 2 Westerns
With Lysate 400µg
Mouse anti Human USP14 antibody recognizes ubiquitin carboxyl-terminal hydrolase 14, also known as deubiquitinating enzyme 14, tRNA-guanine transglycosylase, 60-kD subunit, ubiquitin specific protease 14 (tRNA-guanine transglycosylase), ubiquitin thioesterase 14, ubiquitin thiolesterase 14 and ubiquitin-specific-processing protease 14.

Encoded by the USP14 gene, ubiquitin carboxyl-terminal hydrolase 14 is a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human USP14 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species
Human
Western Blotting
Anti USP14 detects a band of approximately 60 kDa in LNCaP cell lysates
Product Form
Purified IgG - liquid
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Preparation
20μl Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
Purified His-tagged USP14 protein
Instructions For Use
Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition
400μg LNCaP lysate lyophilized in RIPA buffer.
Lysate Reconstitution
- If using DDT reconstitute the lyophilized lysate with 190μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H2O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage
Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Storage
Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life
As supplied, 12 months from date of despatch.
Shelf Life
As supplied, 12 months from date of despatch.

More Information

UniProt
P54578 Related reagents
Entrez Gene
USP14 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0000502 proteasome complex
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0004843 ubiquitin-specific protease activity
GO:0004866 endopeptidase inhibitor activity
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0008193 tRNA guanylyltransferase activity
GO:0061136 regulation of proteasomal protein catabolic process
GO:0009986 cell surface
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0050920 regulation of chemotaxis
GO:0070628 proteasome binding
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements
PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory
For research purposes only

Applications of USP14 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
Western Blotting 1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB
Goat anti Mouse IgG (H/L):HRP STAR207P 2 ml WB

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