USP14 antibody

Mouse anti USP14

Product Type
PrecisionAb Monoclonal
Isotype
IgG1
Format
Purified
Specificity
USP14

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VMA00300
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Mouse anti Human USP14 antibody recognizes ubiquitin carboxyl-terminal hydrolase 14, also known as deubiquitinating enzyme 14, tRNA-guanine transglycosylase, 60-kD subunit, ubiquitin specific protease 14 (tRNA-guanine transglycosylase), ubiquitin thioesterase 14, ubiquitin thiolesterase 14 and ubiquitin-specific-processing protease 14.

Encoded by the USP14 gene, ubiquitin carboxyl-terminal hydrolase 14 is a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized (provided by RefSeq, Jul 2008).

Mouse anti Human USP14 antibody detects a band of 60 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti USP14 detects a band of approximately 60 kDa in LNCaP cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody prepared by affinity chromatography on Protein G from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
Purified His-tagged USP14 protein
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

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UniProt
P54578
Entrez Gene
USP14
GO Terms
GO:0005886 plasma membrane
GO:0000502 proteasome complex
GO:0004197 cysteine-type endopeptidase activity
GO:0004221 ubiquitin thiolesterase activity
GO:0004843 ubiquitin-specific protease activity
GO:0004866 endopeptidase inhibitor activity
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0008193 tRNA guanylyltransferase activity
GO:0061136 regulation of proteasomal protein catabolic process
GO:0009986 cell surface
GO:0016023 cytoplasmic membrane-bounded vesicle
GO:0050920 regulation of chemotaxis
GO:0070628 proteasome binding
View more products with USP14 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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