UBE2M antibody | OTI2D9

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Anti UBE2M Antibody, clone OTI2D9 (PrecisionAb™ Monoclonal Antibody) gallery image 1 — Western blot analysis of whole cell lysates probed with UBE2M antibody followed by detection with HRP conjugated Goat anti Mouse IgG (1/10,000, STAR207P) and visualized on the ChemiDoc MP with 120 second exposure. Arrow points to UBE2M (molecular weight 21 kDa)

Mouse anti UBE2M

UBE2M antibody recognizes the NEDD8-conjugating enzyme Ubc12, also known as NEDD8 carrier protein, NEDD8 protein ligase, ubiquitin carrier protein M, ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), or ubiquitin-protein ligase M.

UBE2M Antibody with Control Lysate

Product Type: PrecisionAb™ Monoclonal
Clone: OTI2D9
Isotype: IgG1
Format: Purified

Product Code	Applications	Pack Size
VMA00144	WB	100 µl
VMA00144KT	WB	2 Westerns
With Lysate		400µg

Summary
Secondary Antibodies

Mouse anti Human UBE2M antibody recognizes UBE2M, also known as NEDD8 carrier protein, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, ubiquitin carrier protein M, ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), ubiquitin-protein ligase M or yeast UBC12 homolog.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. The UBE2M gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin (provided by RefSeq, Jul 2008).

Mouse anti Human UBE2M antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.

Product Details

Target Species: Human
Western Blotting: Anti UBE2M detects a band of approximately 21 kDa in HEK293 cell lysates.
Product Form: Purified IgG - liquid
Product Form: Purified IgG - liquid
Preparation: Mouse monoclonal antibody purified by affinity chromatography from ascites.
Preparation: 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution: Phosphate buffered saline
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
50% Glycerol
Immunogen: Full length recombinant human UBE2M produced in HEK293T cells
Instructions For Use: Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.

Positive Control Lysate

Lysate Composition: 400μg HEK293 lysate lyophilized in RIPA buffer.
Lysate Reconstitution: - If using DDT reconstitute the lyophilized lysate with 190μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 10μl 2M DTT.
- If using BME reconstitute the lyophilized lysate with 180μl DI H₂O, add 200μl 2x Laemmli Sample Buffer and 20μl BME.
Heat at 95°C for 5 minutes. For 10 well mini gels load 25μl. For other gel and comb formats please refer to the PrecisionAb western blotting protocol.

Storage Information

Storage: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.
Storage: Antibody: Store undiluted at -20^oC, avoiding repeated freeze thaw cycles.

Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage.
Shelf Life: As supplied, 12 months from date of despatch.
Shelf Life: As supplied, 12 months from date of despatch.

More Information

UniProt: P61081 Related reagents
Entrez Gene: UBE2M Related reagents
GO Terms: GO:0005515 protein binding; GO:0004842 ubiquitin-protein ligase activity; GO:0005524 ATP binding; GO:0018169 ribosomal S6-glutamic acid ligase activity; GO:0019788 NEDD8 ligase activity; GO:0045116 protein neddylation; GO:0043687 post-translational protein modification; GO:0051246 regulation of protein metabolic process
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Acknowledgements: PrecisionAb™ is a trademark of Bio-Rad Laboratories.
Regulatory: For research purposes only

Applications of UBE2M antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/1000
Western Blotting		1/1000

PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.

Secondary Antibodies Available

Description	Product Code	Pack Size	Applications	List Price	Quantity
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB
Goat anti Mouse IgG (H/L):HRP	STAR207P	2 ml	WB

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