UBE2M antibody | OTI2D9

Mouse anti UBE2M

Product Type
PrecisionAb Monoclonal
Clone
OTI2D9
Isotype
IgG1
Format
Purified
Specificity
UBE2M

Product Code Applications Pack Size List Price Your Price Qty
VMA00144
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl loader
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loader

Mouse anti Human UBE2M antibody recognizes UBE2M, also known as NEDD8 carrier protein, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, ubiquitin carrier protein M, ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), ubiquitin-protein ligase M or yeast UBC12 homolog.

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. The UBE2M gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin (provided by RefSeq, Jul 2008).

Mouse anti Human UBE2M antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Anti UBE2M detects a band of approximately 21 kDa in HEK293 cell lysates.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography from ascites.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol
Immunogen
Full length recombinant human UBE2M produced in HEK293T cells
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

Store undiluted at -20°C, avoiding repeated freeze thaw cycles.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Mouse IgG (H/L):HRP STAR207P WB 2 ml loader
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Description Goat anti Mouse IgG (H/L):HRP

UniProt
P61081
Entrez Gene
UBE2M
GO Terms
GO:0005515 protein binding
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0018169 ribosomal S6-glutamic acid ligase activity
GO:0019788 NEDD8 ligase activity
GO:0045116 protein neddylation
GO:0043687 post-translational protein modification
GO:0051246 regulation of protein metabolic process

VMA00144

240615

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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