UBE2M antibody | OTI2D9
Mouse anti UBE2M
UBE2M antibody recognizes the NEDD8-conjugating enzyme Ubc12, also known as NEDD8 carrier protein, NEDD8 protein ligase, ubiquitin carrier protein M, ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), or ubiquitin-protein ligase M.
UBE2M Antibody with Control Lysate
UBE2M antibody recognizes the NEDD8-conjugating enzyme Ubc12, also known as NEDD8 carrier protein, NEDD8 protein ligase, ubiquitin carrier protein M, ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), or ubiquitin-protein ligase M.
- Product Type
- PrecisionAb™ Monoclonal
- Clone
- OTI2D9
- Isotype
- IgG1
| Product Code | Applications | Datasheet | MSDS | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|---|
| VMA00144 | WB | 100 µl | ||||
| VMA00144KT | WB | 2 Westerns | ||||
| With Lysate | 400µg |
Mouse anti Human UBE2M antibody recognizes UBE2M, also known as NEDD8 carrier protein, NEDD8 protein ligase, NEDD8-conjugating enzyme Ubc12, ubiquitin carrier protein M, ubiquitin-conjugating enzyme E2M (UBC12 homolog, yeast), ubiquitin-protein ligase M or yeast UBC12 homolog.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. The UBE2M gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin (provided by RefSeq, Jul 2008).
Mouse anti Human UBE2M antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. The UBE2M gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin (provided by RefSeq, Jul 2008).
Mouse anti Human UBE2M antibody detects a band of 21 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.
Product Details
- Target Species
- Human
- Product Form
- Purified IgG - liquid
- Product Form
- Purified IgG - liquid
- Preparation
- Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Preparation
- 20μl Mouse monoclonal antibody purified by affinity chromatography from ascites.
- Buffer Solution
- Phosphate buffered saline
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
50% Glycerol - Immunogen
- Full length recombinant human UBE2M produced in HEK293T cells
Storage Information
- Storage
- Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
- Storage
- Antibody: Store undiluted at -20oC, avoiding repeated freeze thaw cycles.
Lysate: Store lyophilized lysate at -20°C. After reconstitution aliquot and store at -20°C for up to 3 months or at -80°C for longer term storage. - Shelf Life
- As supplied, 12 months from date of despatch.
- Shelf Life
- As supplied, 12 months from date of despatch.
More Information
- UniProt
- P61081 Related reagents
- Entrez Gene
- UBE2M Related reagents
- GO Terms
- GO:0005515 protein binding
- GO:0004842 ubiquitin-protein ligase activity
- GO:0005524 ATP binding
- GO:0018169 ribosomal S6-glutamic acid ligase activity
- GO:0019788 NEDD8 ligase activity
- GO:0045116 protein neddylation
- GO:0043687 post-translational protein modification
- GO:0051246 regulation of protein metabolic process
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Acknowledgements
- PrecisionAb™ is a trademark of Bio-Rad Laboratories.
- Regulatory
- For research purposes only
Applications of UBE2M antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/1000 | ||
| Western Blotting | 1/1000 |
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
PrecisionAb antibodies have been extensively validated for the western blot application. The antibody has been validated at the suggested dilution. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependant on sample type.
- Western Blotting
- Anti UBE2M detects a band of approximately 21 kDa in HEK293 cell lysates.
- Western Blotting
- Anti UBE2M detects a band of approximately 21 kDa in HEK293 cell lysates.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
- Instructions For Use
- Please refer to the PrecisionAb western blotting protocol. For additional information on secondary antibody dilution and exposure time see product web page.
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Secondary Antibodies Available
| Description | Product Code | Pack Size | Applications | List Price | Quantity |
|---|---|---|---|---|---|
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB | ||
| Goat anti Mouse IgG (H/L):HRP | STAR207P | 2 ml | WB |
