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UBE2D2 antibody

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Anti UBE2D2 Antibody (PrecisionAb Monoclonal Antibody) thumbnail image 1
Anti UBE2D2 Antibody (PrecisionAb Monoclonal Antibody) gallery image 1
Western blot analysis of whole cell lysates probed with UBE2D2 antibody followed by detection with Goat anti Human IgG F(ab')2:HRP (1/2,500, STAR126) and visualized on the ChemiDoc MP with 1 second exposure. Arrow points to UBE2D2 (molecular weight 18 kDa).

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Human anti UBE2D2

Product Type
PrecisionAb Monoclonal
Isotype
HuCAL Fab bivalent
Format
Purified
Specificity
UBE2D2

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Product Code Applications Pack Size List Price Your Price Qty
VMA00599
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
WB 100 µl
List Price Your Price

Human anti Human UBE2D2 antibody recognizes the ubiquitin-conjugating enzyme E2 D2, also known as p53-regulated ubiquitin-conjugating enzyme 1, ubiquitin carrier protein D2, ubiquitin-conjugating enzyme E2-17 kDa 2 or Ubiquitin-protein ligase D2.

Regulated degradation of misfolded, damaged or short-lived proteins in eukaryotes occurs via the ubiquitin (Ub)-proteasome system (UPS). An integral part of the UPS system is the ubiquitination of target proteins and covalent linkage of Ub-containing proteins to form polymeric chains, marking them as targets for 26S proteasome-mediated degradation. Ubiquitination of proteins is mediated by a cascade of enzymes which includes E1 (ubiquitin activating), E2 (ubiquitin conjugating), and E3 (ubiquitin ligases) enzymes. UBE2D2 encodes a member of the E2 enzyme family. Substrates of this enzyme include the tumor suppressor protein p53 and peroxisomal biogenesis factor 5 (PEX5). Alternative splicing results in multiple transcript variants of UBE2D2 (provided by RefSeq, May 2013).

Human anti Human UBE2D2 antibody detects a band of 18 kDa. The antibody has been extensively validated for western blotting using whole cell lysates.		 PrecisionAb Antibodies

Target Species
Human
Western Blotting
Human anti UBE2D2 antibody detects a band of approximately 18 kDa in NIH/3T3 cell lysates.
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Human monoclonal antibody purified by metal chelate affinity chromatography from E. coli extract.
Source
E.coli
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.09% Sodium Azide (NaN3).
Immunogen
E. coli-derived full length recombinant human UBE2D2
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the protein. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000
The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

Description Product Code Applications Pack Size List Price Your Price Quantity
Goat anti Human IgG F(ab')2:HRP STAR126P E WB 0.5 ml
List Price Your Price
Description Goat anti Human IgG F(ab')2:HRP

UniProt
P62837
Entrez Gene
UBE2D2
GO Terms
GO:0005515 protein binding
GO:0004842 ubiquitin-protein ligase activity
GO:0005524 ATP binding
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0043687 post-translational protein modification
GO:0051246 regulation of protein metabolic process
GO:0070936 protein K48-linked ubiquitination

VMA00599

170508

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