Ubc antibody | AB04/3D2

Mouse anti Ubc

Product Type
PrecisionAb Monoclonal
Clone
AB04/3D2
Isotype
IgG2b
Format
Purified
Specificity
Ubc

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VMA00906
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Mouse anti UBC antibody recognizes polyubiquitin-C.

UBC is important for maintaining homeostatic levels of ubiquitin (Ub), an abundant protein which is one of the most important post-translational modifiers within the proteome. Ubiquitination marks proteins and organelles for degredation or autophagic clearance, essential for normal cellular functions (Senft et al. 2018). UBC is upregulated during stress and downregulated when Ub is overexpressed, and appears to play a protective role in response to stress (Bianchi et al. 2019). The promoter of the UBC gene includes heat-shock elements (HSEs) which explain its upregulation during stress. Accordingly, Ub itself is considered a hest shock protein (Crinelli et al. 2015). Proteasomal degradation, mediated by Ub, is often dysregulated in cancer cells and can lead to tumorigenesis and cancer progression. Furthermore, UBC interacts with many cancer-associated proteins, including CDK1, E2F1, EGFR and p53, and might be involved with regulation of the tumor microenvironment (Kim et al. 2017). Components of the Ub pathway have been suggested as drug targets for cancer therapy (Liu et al. 2016).
PrecisionAb Antibodies

Target Species
Human
Western Blotting
Mouse anti UBC antibody detects a band of approximately 10 kDa in HeLa cell lysates
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Mouse monoclonal antibody purified by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
E. coli-derived recombinant protein, aa 1-304 of human UBC
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Regulatory
For research purposes only
Guarantee
As supplied, 12 months from date of despatch
Acknowledgements
PrecisionAb is a trademark of Bio-Rad Laboratories

Store undiluted at -20oC, avoiding repeated freeze thaw cycles

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/1000 1/2000

The PrecisionAb label is reserved for antibodies that meet the defined performance criteria within Bio-Rad's ongoing antibody validation programme. Click here to learn how we validate our PrecisionAb range. Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Further optimization may be required dependent on sample type.

UniProt
P0CG48
Entrez Gene
UBC
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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